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AB325554

Anti-KCNQ3 antibody [EPR25237-111]

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Rabbit Recombinant Monoclonal KCNQ3 antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human, Mouse, Rat samples.
1 Images
Western blot - Anti-KCNQ3 antibody [EPR25237-111] (AB325554)
  • WB

Lab

Western blot - Anti-KCNQ3 antibody [EPR25237-111] (AB325554)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver, kidney(PMID : 9677360), spleen

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(97kDa).

All lanes:

Western blot - Anti-KCNQ3 antibody [EPR25237-111] (ab325554) at 1/1000 dilution

Lane 1:

Mouse cerebral cortex tissue lysate at 40 µg

Lane 2:

Mouse kidney tissue lysate at 40 µg

Lane 3:

Rat brain tissue lysate at 40 µg

Lane 4:

Rat liver tissue lysate at 40 µg

Lane 5:

Rat spleen tissue lysate at 40 µg

Lane 6:

Rat kidney tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 96 kDa,36 kDa

false

Exposure time: 6s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25237-111

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pore-forming subunit of the voltage-gated potassium (Kv) M-channel which is responsible for the M-current, a key controller of neuronal excitability (PubMed : 16319223, PubMed : 27564677, PubMed : 28793216, PubMed : 9872318). M-channel is composed of pore-forming subunits KCNQ2 and KCNQ3 assembled as heterotetramers (PubMed : 14534157, PubMed : 16319223, PubMed : 27564677, PubMed : 9872318). The native M-current has a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs (PubMed : 14534157, PubMed : 16319223, PubMed : 28793216). M-channel is selectively permeable in vitro to other cations besides potassium, in decreasing order of affinity K(+) > Rb(+) > Cs(+) > Na(+) (PubMed : 28793216). M-channel association with SLC5A3/SMIT1 alters channel ion selectivity, increasing Na(+) and Cs(+) permeation relative to K(+) (PubMed : 28793216). Suppressed by activation of M1 muscarinic acetylcholine receptors (PubMed : 10713961). KCNQ3 also associates with KCNQ5 to form a functional channel in vitro and may also contribute to the M-current in brain (PubMed : 11159685).
See full target information KCNQ3

Alternative Names

Potassium voltage-gated channel subfamily KQT member 3, KQT-like 3, Potassium channel subunit alpha KvLQT3, Voltage-gated potassium channel subunit Kv7.3, KCNQ3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com