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AB302782

Anti-KCNQ3 antibody [EPR25237-57]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal KCNQ3 antibody. Suitable for IHC-P, IHC-Fr, ICC/IF and reacts with Mouse, Rat, Transfected cell line - Mouse samples. Cited in 1 publication.

View Alternative Names

Potassium voltage-gated channel subfamily KQT member 3, KQT-like 3, Potassium channel subunit alpha KvLQT3, Voltage-gated potassium channel subunit Kv7.3, Kcnq3

7 Images
Immunohistochemistry - Anti-KCNQ3 antibody [EPR25237-57] (AB302782)
  • IHC

Supplier Data

Immunohistochemistry - Anti-KCNQ3 antibody [EPR25237-57] (AB302782)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells t tissue labeling KCNQ3 with ab302782 at 1/8000 (0.066 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on (A) HEK-293T cells transfected with a mouse KCNQ3 expression vector containing a his tag. No staining on (B) HEK-293T cells transfected with a mouse KCNQ2 expression vector containing a his tag and (C) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab302782 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry - Anti-KCNQ3 antibody [EPR25237-57] (AB302782)
  • IHC

Supplier Data

Immunohistochemistry - Anti-KCNQ3 antibody [EPR25237-57] (AB302782)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling KCNQ3 with ab302782 at 1/2000 (0.264 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on mouse cerebrum (PMID : 20534576). The section was incubated with ab302782 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry - Anti-KCNQ3 antibody [EPR25237-57] (AB302782)
  • IHC

Supplier Data

Immunohistochemistry - Anti-KCNQ3 antibody [EPR25237-57] (AB302782)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling KCNQ3 with ab302782 at 1/2000 (0.264 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control : no staining on mouse liver (PMID : 9677360). The section was incubated with ab302782 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-KCNQ3 antibody [EPR25237-57] (AB302782)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-KCNQ3 antibody [EPR25237-57] (AB302782)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling KCNQ3 with ab302782 at 1/50 (10.56 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p> .

Immunohistochemistry (Frozen sections) - Anti-KCNQ3 antibody [EPR25237-57] (AB302782)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-KCNQ3 antibody [EPR25237-57] (AB302782)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat heart (fresh) tissue labeling KCNQ3 with ab302782 at 1/50 (10.56 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : no staining on rat heart (PMID : 9677360) is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p> .

Immunocytochemistry - Anti-KCNQ3 antibody [EPR25237-57] (AB302782)
  • ICC

Supplier Data

Immunocytochemistry - Anti-KCNQ3 antibody [EPR25237-57] (AB302782)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling KCNQ3 with ab302782 at 1/500 (1.056 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neural/glia cellConfocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Immunohistochemistry - Anti-KCNQ3 antibody [EPR25237-57] (AB302782)
  • IHC

Supplier Data

Immunohistochemistry - Anti-KCNQ3 antibody [EPR25237-57] (AB302782)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling KCNQ3 with ab302782 at 1/2000 (0.264 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on rat cerebrum (PMID : 20534576). The section was incubated with ab302782 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Carrier free

    Anti-KCNQ3 antibody [EPR25237-57] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25237-57

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-P, IHC-Fr, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pore-forming subunit of the voltage-gated potassium (Kv) M-channel which is responsible for the M-current, a key controller of neuronal excitability. M-channel is composed of pore-forming subunits KCNQ2 and KCNQ3 assembled as heterotetramers (By similarity). The native M-current has a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. M-channel is selectively permeable in vitro to other cations besides potassium, in decreasing order of affinity K(+) > Rb(+) > Cs(+) > Na(+). M-channel association with SLC5A3/SMIT1 alters channel ion selectivity, increasing Na(+) and Cs(+) permeation relative to K(+). Suppressed by activation of M1 muscarinic acetylcholine receptors. KCNQ3 also associates with KCNQ5 to form a functional channel in vitro and may also contribute to the M-current in brain (By similarity).
See full target information Kcnq3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1330167 PubMed38440182

2024

The transmembrane channel-like 6 (TMC6) in primary sensory neurons involving thermal sensation via modulating M channels.

Applications

Unspecified application

Species

Unspecified reactive species

Yating An,Jingyi Hu,Han Hao,Weixin Zhao,Xiaoxue Zhang,Jicheng Shao,Caixue Wang,Xinmeng Li,Chao Liu,Jinsha He,Yiwen Zhao,Hailin Zhang,Xiaona Du
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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