AB84820

Anti-KCNQ4 antibody [N43/6]

Name: Anti-KCNQ4 antibody [N43/6] (ab84820) | Abcam
Brand: Abcam Limited
SKU: AB84820
Price: 520 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(6 Publications)

Mouse Monoclonal KCNQ4 antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Rat, Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Fusion protein corresponding to aa 1-100 of KCNQ4, fused to KCNQ4.

View Alternative Names

Potassium voltage-gated channel subfamily KQT member 4, KQT-like 4, Potassium channel subunit alpha KvLQT4, Voltage-gated potassium channel subunit Kv7.4, KCNQ4

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ4 antibody [N43/6] (AB84820)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ4 antibody [N43/6] (AB84820)

Immunohistochemical analysis of paraffin-embedded human hippocampus tissue labelling KCNQ4 with ab84820 at 1/1000 dilution, for 1 hour at RT followed by secondary antibody Goat Anti-Mouse (FITC) (Green) at 1/50 dilution, for 1 hour at RT.

Immunohistochemistry (Frozen sections) - Anti-KCNQ4 antibody [N43/6] (AB84820)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-KCNQ4 antibody [N43/6] (AB84820)

Immunohistochemical analysis of Mouse brain tissue sections labeling KCNQ4 with ab84820 at 1/1000 dilution for 1 hour at room temperature.

Secondary Antibody : HRP/DAB Detection

System : Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature.

Counterstain : Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 μl for 5 minutes at room temperature

Unknown

Western blot - Anti-KCNQ4 antibody [N43/6] (AB84820)

All lanes:

Western blot - Anti-KCNQ4 antibody [N43/6] (ab84820) at 1/1000 dilution

Lane 1:

Molecular weight marker

Lane 2:

Mixed cell lysates prepared from rat tissues Brain, Heart, Kidney, Liver, Lung, Pancreas, Skeletal Muscle, Spleen, Testes, Thymus, and H9C2 cells at 15 µg

Secondary

All lanes:

Sheep Anti-Mouse IgG: HRP

Predicted band size: 77 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N43/6

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, IHC-Fr

applications

Immunogen

Fusion protein corresponding to aa 1-100 of KCNQ4, fused to KCNQ4. The exact immunogen used to generate this antibody is proprietary information.

P56696

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "Fixation: Bouin's Fixative", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/1000", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

The clone number has been updated from S43-6 to N43/6, both clone numbers name the same antibody clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pore-forming subunit of the voltage-gated potassium (Kv) channel involved in the regulation of sensory cells excitability in the cochlea (PubMed : 10025409, PubMed : 34767770). KCNQ4/Kv7.4 channel is composed of 4 pore-forming subunits assembled as tetramers (PubMed : 34767770). Promotes the outflow of potassium ions in the repolarization phase of action potential which plays a role in regulating membrane potential of excitable cells (PubMed : 10025409, PubMed : 11245603, PubMed : 34767770). The channel conducts a slowly activating and deactivating current (PubMed : 10025409, PubMed : 11245603). Current often shows some inward rectification at positive potentials (PubMed : 10025409). Channel may be selectively permeable in vitro to other cations besides potassium, in decreasing order of affinity K(+) = Rb(+) > Cs(+) > Na(+) (PubMed : 10025409). Important for normal physiological function of inner ear such as sensory perception of sound (PubMed : 10025409, PubMed : 10369879).

See full target information KCNQ4

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:15260 PubMed38956136

2024

Vestibular hair cells are more prone to damage by excessive acceleration insult in the mouse with KCNQ4 dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Hansol Hong,Eun Ji Koo,Yesai Park,Gabae Song,Sun Young Joo,Jung Ah Kim,Heon Yung Gee,Jinsei Jung,Kangyoon Park,Gyu Cheol Han,Jae Young Choie,Sung Huhn Kim

eLife 11: PubMed36125121

2022

Repressor element 1-silencing transcription factor deficiency yields profound hearing loss through K7.4 channel upsurge in auditory neurons and hair cells.

Applications

Unspecified application

Species

Unspecified reactive species

Haiwei Zhang,Hongchen Li,Mingshun Lu,Shengnan Wang,Xueya Ma,Fei Wang,Jiaxi Liu,Xinyu Li,Haichao Yang,Fan Zhang,Haitao Shen,Noel J Buckley,Nikita Gamper,Ebenezer N Yamoah,Ping Lv

The journal of physiological sciences : JPS 71:28 PubMed34479475

2021

miR-153/KCNQ4 axis contributes to noise-induced hearing loss in a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Wang,Wei Li,Cuiyun Cai,Peng Hu,Ruosha Lai

Journal of the Association for Research in Otolary 20:217-232 PubMed30710318

2019

Inhibition of Histone Methyltransferase G9a Attenuates Noise-Induced Cochlear Synaptopathy and Hearing Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Xiong,Haishan Long,Song Pan,Ruosha Lai,Xianren Wang,Yuanping Zhu,Kayla Hill,Qiaojun Fang,Yiqing Zheng,Su-Hua Sha

The Journal of biological chemistry 289:2099-111 PubMed24297175

2013

Differential protein kinase C-dependent modulation of Kv7.4 and Kv7.5 subunits of vascular Kv7 channels.

Applications

ICC/IF, WB

Species

Unspecified reactive species, Unspecified reactive species

Lioubov I Brueggemann,Alexander R Mackie,Leanne L Cribbs,Jessica Freda,Abhishek Tripathi,Matthias Majetschak,Kenneth L Byron

Neuroscience 215:209-16 PubMed22546335

2012

Inwardly rectifying potassium channel Kir4.1 is localized at the calyx endings of vestibular afferents.

Applications

Unspecified application

Species

Mouse

T Udagawa,N Tatsumi,T Tachibana,Y Negishi,H Saijo,T Kobayashi,Y Yaguchi,H Kojima,H Moriyama,M Okabe

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KCNQ4 antibody [N43/6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ4 antibody [N43/6] (AB84820)

Immunohistochemistry (Frozen sections) - Anti-KCNQ4 antibody [N43/6] (AB84820)

Western blot - Anti-KCNQ4 antibody [N43/6] (AB84820)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (6)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise