Mouse Monoclonal KCNT1/SLACK antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Rat, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Kcnt1 aa 1150 to C-terminus.
Images
![Western blot - Anti-KCNT1/SLACK antibody [N3/26] (AB94578), expandable thumbnail](https://content.abcam.com/products/images/kcnt1-slack-antibody-n3-26-ab94578--western-blot-img32693.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)")
![Flow Cytometry - Anti-KCNT1/SLACK antibody [N3/26] (AB94578), expandable thumbnail](https://content.abcam.com/products/images/kcnt1-slack-antibody-n3-26-ab94578--flow-cytometry-img121505.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [N3/26] (AB94578), expandable thumbnail](https://content.abcam.com/products/images/kcnt1-slack-antibody-n3-26-ab94578--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img1725.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)")
Publications
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- eLife 12:2023Tiered sympathetic control of cardiac function revealed by viral tracing and single cell transcriptome profiling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSachin Sharma,Russell Littman,John D Tompkins,Douglas Arneson,Jaime Contreras,Al-Hassan Dajani,Kaitlyn Ang,Amit Tsanhani,Xin Sun,Patrick Y Jay,Herbert Herzog,Xia Yang,Olujimi A AjijolaPubMed 37162194
- Neuroscience bulletin 37:669-6832021A Role for Transmembrane Protein 16C/Slack Impairment in Excitatory Nociceptive Synaptic Plasticity in the Pathogenesis of Remifentanil-induced Hyperalgesia in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYize Li,Linlin Zhang,Jing Li,Chunyan Wang,Yi Chen,Yuan Yuan,Keliang Xie,Guolin Wang,Yonghao YuPubMed 33779892
- Cell reports 33:1083032020Reduced GABAergic Neuron Excitability, Altered Synaptic Connectivity, and Seizures in a KCNT1 Gain-of-Function Mouse Model of Childhood Epilepsy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmy N Shore,Sophie Colombo,William F Tobin,Sabrina Petri,Erin R Cullen,Soledad Dominguez,Christopher D Bostick,Michael A Beaumont,Damian Williams,Dion Khodagholy,Mu Yang,Cathleen M Lutz,Yueqing Peng,Jennifer N Gelinas,David B Goldstein,Michael J Boland,Wayne N Frankel,Matthew C WestonPubMed 33113364
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Rat Kcnt1 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Z258
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected | Expected |
| Mouse | Expected | Expected | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Outwardly rectifying potassium channel subunit that may coassemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro) (By similarity).
Alternative names
KIAA1422, KCNT1, Potassium channel subfamily T member 1, KCa4.1
Recommended products
Mouse Monoclonal KCNT1/SLACK antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Rat, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Kcnt1 aa 1150 to C-terminus.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Rat Kcnt1 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Z258
- Clone number
- N3/26
- Purification technique
- Affinity purification Protein G
- Specificity
Detects ~140kDa. Weak human detection. Does not cross-react with KCNT2/Slo2.1/Slick.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The clone number has been updated from S3-26 to N3/26, both clone numbers name the same antibody clone.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
![Western blot - Anti-KCNT1/SLACK antibody [N3/26] (ab94578), expandable thumbnail](https://content.abcam.com/products/images/kcnt1-slack-antibody-n3-26-ab94578--western-blot-img32693.jpg "Western blot - Anti-KCNT1/SLACK antibody [N3/26] (ab94578)")
Western blot - Anti-KCNT1/SLACK antibody [N3/26] (ab94578)
Membrane was blocked with 1.5% BSA for 30 minutes at room temperature. Primari antibody was incubated for 2 hours at room temperature.
All lanes: Western blot - Anti-KCNT1/SLACK antibody [N3/26] (ab94578) at 1/1000 dilution
All lanes: Rat brain lysate at 15 µg
SecondaryAll lanes: HRP-conjugated sheet anti-mouse IgG
Predicted band size: 138 kDa
![Flow Cytometry - Anti-KCNT1/SLACK antibody [N3/26] (ab94578), expandable thumbnail](https://content.abcam.com/products/images/kcnt1-slack-antibody-n3-26-ab94578--flow-cytometry-img121505.jpg "Flow Cytometry - Anti-KCNT1/SLACK antibody [N3/26] (ab94578)")
Flow Cytometry - Anti-KCNT1/SLACK antibody [N3/26] (ab94578)
Overlay histogram showing SH-SY5Y cells stained with ab94578 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab94578, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [N3/26] (ab94578), expandable thumbnail](https://content.abcam.com/products/images/kcnt1-slack-antibody-n3-26-ab94578--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img1725.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [N3/26] (ab94578)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [N3/26] (ab94578)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse backskin staining KCNT1/SLACK with ab94578 at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
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