AB94578

Anti-KCNT1/SLACK antibody [N3/26]

Name: Anti-KCNT1/SLACK antibody [N3/26] (ab94578) | Abcam
Brand: Abcam Limited
SKU: AB94578
Price: 500 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(4 Publications)

Anti-KCNT1/SLACK antibody [N3/26] (ab94578) is a mouse monoclonal antibody detecting KCNT1/SLACK in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.

- Trusted since 2010

View Alternative Names

KIAA1422, KCNT1, Potassium channel subfamily T member 1, KCa4.1

3 Images

Supplier Data

Western blot - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

Membrane was blocked with 1.5% BSA for 30 minutes at room temperature. Primari antibody was incubated for 2 hours at room temperature.

All lanes:

Western blot - Anti-KCNT1/SLACK antibody [N3/26] (ab94578) at 1/1000 dilution

All lanes:

Rat brain lysate at 15 µg

Secondary

All lanes:

HRP-conjugated sheet anti-mouse IgG

Predicted band size: 138 kDa

false

Flow Cytometry - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

Flow Cyt

Unknown

Flow Cytometry - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

Overlay histogram showing SH-SY5Y cells stained with ab94578 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab94578, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse backskin staining KCNT1/SLACK with ab94578 at 1 : 100 for 1 hour at RT. Secondary Antibody : FITC Goat Anti-Mouse (green) at 1 : 50 for 1 hour at RT.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N3/26

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF, Flow Cyt, IHC-Fr

applications

Immunogen

Recombinant Fragment Protein within Rat Kcnt1 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Z258

Specificity

Detects ~140kDa. Weak human detection. Does not cross-react with KCNT2/Slo2.1/Slick.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-KCNT1/SLACK antibody [N3/26] (ab94578) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of KCNT1/SLACK?
Anti-KCNT1/SLACK [N3/26] (ab94578) specifically detects a band for KCNT1/SLACK (UniProt: Q9Z258) at a molecular weight of 137kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Outwardly rectifying potassium channel subunit that may coassemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro) (By similarity).

See full target information KCNT1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed37162194

2023

Tiered sympathetic control of cardiac function revealed by viral tracing and single cell transcriptome profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Sachin Sharma,Russell Littman,John D Tompkins,Douglas Arneson,Jaime Contreras,Al-Hassan Dajani,Kaitlyn Ang,Amit Tsanhani,Xin Sun,Patrick Y Jay,Herbert Herzog,Xia Yang,Olujimi A Ajijola

Neuroscience bulletin 37:669-683 PubMed33779892

2021

A Role for Transmembrane Protein 16C/Slack Impairment in Excitatory Nociceptive Synaptic Plasticity in the Pathogenesis of Remifentanil-induced Hyperalgesia in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yize Li,Linlin Zhang,Jing Li,Chunyan Wang,Yi Chen,Yuan Yuan,Keliang Xie,Guolin Wang,Yonghao Yu

Cell reports 33:108303 PubMed33113364

2020

Reduced GABAergic Neuron Excitability, Altered Synaptic Connectivity, and Seizures in a KCNT1 Gain-of-Function Mouse Model of Childhood Epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Amy N Shore,Sophie Colombo,William F Tobin,Sabrina Petri,Erin R Cullen,Soledad Dominguez,Christopher D Bostick,Michael A Beaumont,Damian Williams,Dion Khodagholy,Mu Yang,Cathleen M Lutz,Yueqing Peng,Jennifer N Gelinas,David B Goldstein,Michael J Boland,Wayne N Frankel,Matthew C Weston

Neurobiology of learning and memory 155:173-179 PubMed30053575

2018

Neocortical SHANK1 regulation of forebrain dependent associative learning.

Applications

Unspecified application

Species

Unspecified reactive species

Sean M Collins,Roberto Galvez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KCNT1/SLACK antibody [N3/26]

Western blot - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

Flow Cytometry - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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