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AB192259

Anti-KCTD21 antibody [EPR12873(2)]

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(2 Publications)

Rabbit Recombinant Monoclonal KCTD21 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 2 publications.

View Alternative Names

BTB/POZ domain-containing protein KCTD21, KCASH2 protein, Potassium channel tetramerization domain-containing protein 21, KCTD21

4 Images
Western blot - Anti-KCTD21 antibody [EPR12873(2)] (AB192259)
  • WB

Supplier Data

Western blot - Anti-KCTD21 antibody [EPR12873(2)] (AB192259)

All lanes:

Western blot - Anti-KCTD21 antibody [EPR12873(2)] (ab192259) at 1/1000 dilution

All lanes:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

false

Flow Cytometry (Intracellular) - Anti-KCTD21 antibody [EPR12873(2)] (AB192259)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-KCTD21 antibody [EPR12873(2)] (AB192259)

Intracellular Flow Cytometry analysis of Caco-2 (Human colorectal adenocarcinoma cell line) cells labeling KCTD21 using ab192259 at a 1/220 dilution (pink). Goat anti-rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

Western blot - Anti-KCTD21 antibody [EPR12873(2)] (AB192259)
  • WB

Supplier Data

Western blot - Anti-KCTD21 antibody [EPR12873(2)] (AB192259)

All lanes:

Western blot - Anti-KCTD21 antibody [EPR12873(2)] (ab192259) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Lane 3:

C6 cell lysate at 10 µg

Lane 4:

RAW264.7 cell lysate at 10 µg

Lane 5:

PC-12 cell lysate at 10 µg

Lane 6:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

false

Western blot - Anti-KCTD21 antibody [EPR12873(2)] (AB192259)
  • WB

Supplier Data

Western blot - Anti-KCTD21 antibody [EPR12873(2)] (AB192259)

All lanes:

Western blot - Anti-KCTD21 antibody [EPR12873(2)] (ab192259) at 1/1000 dilution

All lanes:

human fetal brain lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 30 kDa

false

  • Carrier free

    Anti-KCTD21 antibody [EPR12873(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12873(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/220", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex mediating the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes the ubiquitination of HDAC1. Can function as antagonist of the Hedgehog pathway by affecting the nuclear transfer of transcription factor GLI1; the function probably occurs via HDAC1 down-regulation, keeping GLI1 acetylated and inactive. Inhibits cell growth and tumorigenicity of medulloblastoma (MDB) (PubMed : 21472142).
See full target information BTB/POZ domain-containing protein KCTD21
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:638508 PubMed33898425

2021

Specific Protein 1 and p53 Interplay Modulates the Expression of the KCTD-Containing Cullin3 Adaptor Suppressor of Hedgehog 2.

Applications

Unspecified application

Species

Unspecified reactive species

Annapaola Angrisani,Annamaria Di Fiore,Claudia Augusta Di Trani,Simone Fonte,Marialaura Petroni,Ludovica Lospinoso Severini,Fabio Bordin,Laura Belloni,Elisabetta Ferretti,Gianluca Canettieri,Marta Moretti,Enrico De Smaele

Oncogenesis 8:64 PubMed31685809

2019

KCTD15 inhibits the Hedgehog pathway in Medulloblastoma cells by increasing protein levels of the oncosuppressor KCASH2.

Applications

Unspecified application

Species

Unspecified reactive species

Eleonora Spiombi,Annapaola Angrisani,Simone Fonte,Giuseppina De Feudis,Francesca Fabretti,Danilo Cucchi,Mariapaola Izzo,Paola Infante,Evelina Miele,Agnese Po,Laura Di Magno,Roberto Magliozzi,Daniele Guardavaccaro,Marella Maroder,Gianluca Canettieri,Giuseppe Giannini,Elisabetta Ferretti,Alberto Gulino,Lucia Di Marcotullio,Marta Moretti,Enrico De Smaele
View all publications
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Product promise

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