AB2898

Anti-KDEL antibody

Name: Anti-KDEL antibody (ab2898) | Abcam
Brand: Abcam Limited
SKU: AB2898
Rating: 4 (1 reviews)

(1 Review)

(8 Publications)

Rabbit Polyclonal KDEL antibody. Suitable for IHC-P, ICC/IF, Flow Cyt and reacts with Mouse, Human samples. Cited in 8 publications.

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody (AB2898)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody (AB2898)

Immunofluorescent analysis of U-251 MG (Human brain glioma cell line) cells labeling KDEL (green) with ab2898. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2898 at 1/200 dilution overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Flow Cytometry - Anti-KDEL antibody (AB2898)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-KDEL antibody (AB2898)

Flow cytometry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling KDEL with ab2898 (purple) or a rabbit IgG isotype control (black) at a 10 μg/mL. After incubation for 1 hour on ice, the cells were labeled with a Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 647 conjugate at 1/50 dilution for 1 hour on ice. A representative 10,000 cells were acquired and analyzed for each sample.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (AB2898)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (AB2898)

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse liver tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 100 with a rabbit polyclonal antibody recognizing KDEL ab2898 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (AB2898)

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse pancreas tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 100 with a rabbit polyclonal antibody recognizing KDEL ab2898 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (AB2898)

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 200 with a rabbit polyclonal antibody recognizing KDEL ab2898 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-20 µg/mL", "FlowCyt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

This antibody can be used as an endoplasmic reticulum (ER) marker.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information KDEL

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Life metabolism 3:loae004 PubMed39872215

2025

Long-chain acyl-CoA synthetase regulates systemic lipid homeostasis via glycosylation-dependent lipoprotein production.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Yue Dong,Tianxing Zhou,He Tian,Xiahe Huang,Yong Q Zhang,Yingchun Wang,Sin Man Lam,Guanghou Shui

Cell death & disease 14:297 PubMed37120609

2023

Perturbation of the host cell Ca homeostasis and ER-mitochondria contact sites by the SARS-CoV-2 structural proteins E and M.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Poggio,Francesca Vallese,Andreas J W Hartel,Travis J Morgenstern,Scott A Kanner,Oliver Rauh,Flavia Giamogante,Lucia Barazzuol,Kenneth L Shepard,Henry M Colecraft,Oliver Biggs Clarke,Marisa Brini,Tito Calì

Cells 11: PubMed35626680

2022

Stable Integration of Inducible SPLICS Reporters Enables Spatio-Temporal Analysis of Multiple Organelle Contact Sites upon Modulation of Cholesterol Traffic.

Applications

Unspecified application

Species

Unspecified reactive species

Flavia Giamogante,Lucia Barazzuol,Elena Poggio,Marta Tromboni,Marisa Brini,Tito Calì

The Journal of biological chemistry 298:101904 PubMed35398096

2022

Carbamazepine promotes surface expression of mutant Kir6.2-A28V ATP-sensitive potassium channels by modulating Golgi retention and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Han Lin,Yu-Chi Lin,Shi-Bing Yang,Pei-Chun Chen

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22228 PubMed35218571

2022

TIMP2 mediates endoplasmic reticulum stress contributing to sepsis-induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Nanhui Jiang,Rong Huang,Jiahao Zhang,Dongxue Xu,Tianlong Li,Zhongyi Sun,Lianjiu Su,Zhiyong Peng

The international journal of biochemistry & cell b 113:67-74 PubMed31185295

2019

N-Glycan-calnexin interactions in human factor VII secretion and deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wang,Lina Wang,Shuo Li,Ningzheng Dong,Qingyu Wu

Journal of proteomics 75:2855-68 PubMed22270009

2012

Proteomic analysis of human hepatoma cells expressing methionine adenosyltransferase I/III: Characterization of DDX3X as a target of S-adenosylmethionine.

Applications

Unspecified application

Species

Unspecified reactive species

Paul C Schröder,Joaquín Fernández-Irigoyen,Emilie Bigaud,Antonio Serna,Rubén Renández-Alcoceba,Shelly C Lu,José M Mato,Jesús Prieto,Fernando J Corrales

Molecular & cellular proteomics : MCP 10:M111.009126 PubMed21467216

2011

HSV-1 Cgal+ infection promotes quaking RNA binding protein production and induces nuclear-cytoplasmic shuttling of quaking I-5 isoform in human hepatoma cells.

Applications

Species

Unspecified reactive species

Virginia Sánchez-Quiles,María I Mora,Victor Segura,Anna Greco,Alberto L Epstein,Maria Giovanna Foschini,Loïc Dayon,Jean-Charles Sanchez,Jesús Prieto,Fernando J Corrales,Enrique Santamaría

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KDEL antibody

Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody (AB2898)

Flow Cytometry - Anti-KDEL antibody (AB2898)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (AB2898)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (AB2898)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (AB2898)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (8)

Product promise