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Mouse Multiclonal KDM1/LSD1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KDM1A.

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Images

Western blot - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (AB31954), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (AB31954), expandable thumbnail
  • Western blot - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (AB31954), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (AB31954), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • Recombinant Fragment Protein within Human KDM1A. The exact immunogen used to generate this antibody is proprietary information. Database link O60341

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context (PubMed:15620353, PubMed:15811342, PubMed:16079794, PubMed:16079795, PubMed:16140033, PubMed:16223729, PubMed:27292636). Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed (PubMed:15620353, PubMed:15811342, PubMed:16079794, PubMed:21300290). Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me (PubMed:15620353, PubMed:20389281, PubMed:21300290, PubMed:23721412). May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity (PubMed:16079794, PubMed:16140033, PubMed:16885027, PubMed:21300290, PubMed:23721412). Also acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in AR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A (PubMed:16079795). Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1 (PubMed:29691401). Demethylates methylated 'Lys-42' and methylated 'Lys-117' of SOX2 (PubMed:29358331). Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (PubMed:16079794, PubMed:16140033). Facilitates epithelial-to-mesenchymal transition by acting as an effector of SNAI1-mediated transcription repression of epithelial markers E-cadherin/CDH1, CDN7 and KRT8 (PubMed:20562920, PubMed:27292636). Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7 (PubMed:20389281).

Alternative names

AOF2, KDM1, KIAA0601, LSD1, KDM1A, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A

Recommended products

Mouse Multiclonal KDM1/LSD1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KDM1A.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Multiclonal
Immunogen
  • Recombinant Fragment Protein within Human KDM1A. The exact immunogen used to generate this antibody is proprietary information. Database link O60341
Clone number
1B2E5 + 1B2F2
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

Purified from tissue culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

Product promise

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4 product images

  • Western blot - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954), expandable thumbnail

    Western blot - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: KDM1/LSD1 knockout HAP1 cell lysate (20 μg)
    Lane 3: HeLa cell lysate (20 μg)
    Lane 4: Jurkat cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab31954 observed at 110 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.


    ab31954 was shown to specifically recognize KDM1/LSD1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when KDM1/LSD1 knockout samples were used. Wild-type and KDM1/LSD1 knockout samples were subjected to SDS-PAGE. ab31954 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (loading control to GAPDH) were diluted 1/250 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

    All lanes: Western blot - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

    Predicted band size: 92 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing nuclear localisation using LSD1 antibody (ab31954) dilution 1:500-1:2000 and an HRP-conjugated secondary antibody with DAB staining.

  • Western blot - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954), expandable thumbnail

    Western blot - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

    KDM1/LSD1 Western blot staining using mouse Anti-KDM1/LSD1 antibody

    Primary antibody dilution range 1:500-1:2000.

    All lanes: Western blot - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

    Lane 1: Molecular weight marker

    Lane 2: LSD1 recombinant protein (NB: truncated form)

    Lane 3: HeLa cell lysate

    Predicted band size: 92 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody [1B2E5 + 1B2F2] (ab31954)

    Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue, showing nuclear localisation using LSD1 antibody (ab31954) dilution 1:500-1:2000 and an HRP-conjugated secondary antibody with DAB staining.

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Product protocols

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