AB62582

Anti-KDM1/LSD1 antibody

Name: Anti-KDM1/LSD1 antibody (ab62582) | Abcam
Brand: Abcam Limited
SKU: AB62582
Price: 535 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal KDM1/LSD1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human KDM1A.

View Alternative Names

AOF2, KDM1, KIAA0601, LSD1, KDM1A, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody (AB62582)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody (AB62582)

Immunofluorescence of LSD1 in Human Small Intestine cells using ab62582 at 20 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody (AB62582)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody (AB62582)

Immunohistochemical analysis of paraffin-embedded human small intestine tissue using ab62582 at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Supplier Data

Western blot - Anti-KDM1/LSD1 antibody (AB62582)

Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KDM1/LSD1 antibody (ab62582) at 1 µg/mL

Lane 1:

MCF7 (human breast adenocarcinoma cell line) cell lysate at 15 µg

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG (HRP) at 1/10000 dilution

Predicted band size: 92 kDa

Observed band size: 110 kDa

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Unknown

Western blot - Anti-KDM1/LSD1 antibody (AB62582)

Lane 1:

Western blot - Anti-KDM1/LSD1 antibody (ab62582) at 1 µg/mL

Lane 2:

Western blot - Anti-KDM1/LSD1 antibody (ab62582) at 2 µg/mL

All lanes:

P815 cell lysate at 15 µg

Predicted band size: 92 kDa

Observed band size: 105 kDa,60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human KDM1A. The exact immunogen used to generate this antibody is proprietary information.

O60341

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context (PubMed : 15620353, PubMed : 15811342, PubMed : 16079794, PubMed : 16079795, PubMed : 16140033, PubMed : 16223729, PubMed : 27292636). Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed (PubMed : 15620353, PubMed : 15811342, PubMed : 16079794, PubMed : 21300290). Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me (PubMed : 15620353, PubMed : 20389281, PubMed : 21300290, PubMed : 23721412). May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity (PubMed : 16079794, PubMed : 16140033, PubMed : 16885027, PubMed : 21300290, PubMed : 23721412). Also acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in AR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A (PubMed : 16079795). Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1 (PubMed : 29691401). Demethylates methylated 'Lys-42' and methylated 'Lys-117' of SOX2 (PubMed : 29358331). Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (PubMed : 16079794, PubMed : 16140033). Facilitates epithelial-to-mesenchymal transition by acting as an effector of SNAI1-mediated transcription repression of epithelial markers E-cadherin/CDH1, CDN7 and KRT8 (PubMed : 20562920, PubMed : 27292636). Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7 (PubMed : 20389281).

See full target information KDM1A

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 38:e23864 PubMed39318028

2024

USP1-mediated deubiquitination of KDM1A promotes the malignant progression of triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Su,Yan Du,Wenguang He

Molecular medicine (Cambridge, Mass.) 28:134 PubMed36401176

2022

CircRNA AFF4 induced by KDM1A promotes osteogenic differentiation through FNDC5/Irisin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ansong Liu,Yong Chen,Da Zhong,Chenggong Wang,Mi Yu,Chao Liu,Zhijun Yang,Wenkang Chen,Ke Yin

American journal of translational research 12:2379-2395 PubMed32655778

2020

Two-layer regulation of mediated by both TLR4/NF-kB signaling and miR-589-5p increases proinflammatory cytokines in the pathology of severe acute pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Chen,Wei-Hua Dong,Qi Wu,Jun Wang

Appetite 99:193-199 PubMed26785315

2016

Programmed hyperphagia in offspring of obese dams: Altered expression of hypothalamic nutrient sensors, neurogenic factors and epigenetic modulators.

Applications

Unspecified application

Species

Unspecified reactive species

Mina Desai,Guang Han,Michael G Ross

Proceedings of the National Academy of Sciences of 112:13627-32 PubMed26487685

2015

Regulation of DLK1 by the maternally expressed miR-379/miR-544 cluster may underlie callipyge polar overdominance inheritance.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Qian Gao,Xin Chen,Pei Wang,Lei Lu,Wei Zhao,Chen Chen,Cai-Ping Chen,Tao Tao,Jie Sun,Yan-Yan Zheng,Jie Du,Chao-Jun Li,Zhen-Ji Gan,Xiang Gao,Hua-Qun Chen,Min-Sheng Zhu

The Journal of comparative neurology 521:833-59 PubMed22847514

2012

Global expression profiling of globose basal cells and neurogenic progression within the olfactory epithelium.

Applications

Species

Mouse

Richard C Krolewski,Adam Packard,James E Schwob

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KDM1/LSD1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody (AB62582)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM1/LSD1 antibody (AB62582)

Western blot - Anti-KDM1/LSD1 antibody (AB62582)

Western blot - Anti-KDM1/LSD1 antibody (AB62582)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (6)

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