Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721) is a rabbit polyclonal antibody detecting KDM1/LSD1 in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat.
- KO validated for confirmed specificity
- Over 220 publications
- Trusted since 2005
Images
Publications
Filter by
- Nature communications 14:72432023Release of Histone H3K4-reading transcription factors from chromosomes in mitosis is independent of adjacent H3 phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRebecca J Harris,Maninder Heer,Mark D Levasseur,Tyrell N Cartwright,Bethany Weston,Jennifer L Mitchell,Jonathan M Coxhead,Luke Gaughan,Lisa Prendergast,Daniel Rico,Jonathan M G HigginsPubMed 37945563
- Journal of translational medicine 21:7522023JMJD6-BRD4 complex stimulates lncRNA HOTAIR transcription by binding to the promoter region of HOTAIR and induces radioresistance in liver cancer stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuifeng Pei,Le Zhao,Yiren Ding,Zhan Su,Deqiang Li,Shuo Zhu,Lu Xu,Wei Zhao,Wuyuan ZhouPubMed 37880710
- The FEBS journal 290:5395-54102023Neutrophil extracellular traps boost laser-induced mouse choroidal neovascularization through the activation of the choroidal endothelial cell TLR4/HIF-1α pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Zeng,Ying Wang,Manhui Zhu,Min Wu,Yamei Zhou,Qiaoyun Wang,Yiqian Xu,Fei Lin,Jiaqi Wang,Yuxuan Li,Shuang Liang,Ziyu Wang,Laiqing Xie,Xiaojuan LiuPubMed 37552110
- Cancer research communications 3:1716-17302023Demethylation of EHMT1/GLP Protein Reprograms Its Transcriptional Activity and Promotes Prostate Cancer Progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Besschetnova,Wanting Han,Mingyu Liu,Yanfei Gao,Muqing Li,Zifeng Wang,Maryam Labaf,Susan Patalano,Kavita Venkataramani,Rachel E Muriph,Jill A Macoska,Kellee R Siegfried,Jason Evans,Steven P Balk,Shuai Gao,Dong Han,Changmeng CaiPubMed 37663929
- Nature communications 14:49442023Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Zeng,Jiwei Chen,Emmalee W Cooke,Arijita Subuddhi,Eliana T Roodman,Fei Xavier Chen,Kaixiang CaoPubMed 37607921
- Nature 619:176-1832023Epigenetic dysregulation from chromosomal transit in micronuclei.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlbert S Agustinus,Duaa Al-Rawi,Bhargavi Dameracharla,Ramya Raviram,Bailey S C L Jones,Stephanie Stransky,Lorenzo Scipioni,Jens Luebeck,Melody Di Bona,Danguole Norkunaite,Robert M Myers,Mercedes Duran,Seongmin Choi,Britta Weigelt,Shira Yomtoubian,Andrew McPherson,Eléonore Toufektchan,Kristina Keuper,Paul S Mischel,Vivek Mittal,Sohrab P Shah,John Maciejowski,Zuzana Storchova,Enrico Gratton,Peter Ly,Dan Landau,Mathieu F Bakhoum,Richard P Koche,Simone Sidoli,Vineet Bafna,Yael David,Samuel F BakhoumPubMed 37286593
- Experimental & molecular medicine 55:1232-12462023SIRT1 ubiquitination is regulated by opposing activities of APC/C-Cdh1 and AROS during stress-induced premature senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSang Hyup Lee,Ji-Hye Yang,Ui-Hyun Park,Hanbyeul Choi,Yoo Sung Kim,Bo-Eun Yoon,Hye-Jeong Han,Hyun-Taek Kim,Soo-Jong Um,Eun-Joo KimPubMed 37258580
- The Journal of clinical investigation 133:2023Nutrient regulation of the islet epigenome controls adaptive insulin secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew Wortham,Fenfen Liu,Austin R Harrington,Johanna Y Fleischman,Martina Wallace,Francesca Mulas,Medhavi Mallick,Nicholas K Vinckier,Benjamin R Cross,Joshua Chiou,Nisha A Patel,Yinghui Sui,Carolyn McGrail,Yesl Jun,Gaowei Wang,Ulupi S Jhala,Roland Schüle,Orian S Shirihai,Mark O Huising,Kyle J Gaulton,Christian M Metallo,Maike SanderPubMed 36821378
- Cancer research 83:1684-16982023LSD1 Inhibition Disrupts Super-Enhancer-Driven Oncogenic Transcriptional Programs in Castration-Resistant Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuqing Li,Mingyu Liu,Wanting Han,Zifeng Wang,Dong Han,Susan Patalano,Jill A Macoska,Steven P Balk,Housheng Hansen He,Eva Corey,Shuai Gao,Changmeng CaiPubMed 36877164
- Nature communications 14:6032023LSD1/PRMT6-targeting gene therapy to attenuate androgen receptor toxic gain-of-function ameliorates spinobulbar muscular atrophy phenotypes in flies and mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRamachandran Prakasam,Angela Bonadiman,Roberta Andreotti,Emanuela Zuccaro,Davide Dalfovo,Caterina Marchioretti,Debasmita Tripathy,Gianluca Petris,Eric N Anderson,Alice Migazzi,Laura Tosatto,Anna Cereseto,Elena Battaglioli,Gianni Sorarù,Wooi Fang Lim,Carlo Rinaldi,Fabio Sambataro,Naemeh Pourshafie,Christopher Grunseich,Alessandro Romanel,Udai Bhan Pandey,Andrea Contestabile,Giuseppe Ronzitti,Manuela Basso,Maria PennutoPubMed 36746939
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context (PubMed:15620353, PubMed:15811342, PubMed:16079794, PubMed:16079795, PubMed:16140033, PubMed:16223729, PubMed:27292636). Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed (PubMed:15620353, PubMed:15811342, PubMed:16079794, PubMed:21300290). Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me (PubMed:15620353, PubMed:20389281, PubMed:21300290, PubMed:23721412). May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity (PubMed:16079794, PubMed:16140033, PubMed:16885027, PubMed:21300290, PubMed:23721412). Also acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in AR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A (PubMed:16079795). Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1 (PubMed:29691401). Demethylates methylated 'Lys-42' and methylated 'Lys-117' of SOX2 (PubMed:29358331). Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (PubMed:16079794, PubMed:16140033). Facilitates epithelial-to-mesenchymal transition by acting as an effector of SNAI1-mediated transcription repression of epithelial markers E-cadherin/CDH1, CDN7 and KRT8 (PubMed:20562920, PubMed:27292636). Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7 (PubMed:20389281).
Alternative names
AOF2, KDM1, KIAA0601, LSD1, KDM1A, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A
Recommended products
Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721) is a rabbit polyclonal antibody detecting KDM1/LSD1 in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat.
- KO validated for confirmed specificity
- Over 220 publications
- Trusted since 2005
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Replenishment batches of our polyclonal antibody, ab17721 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab129195.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of KDM1/LSD1?
Anti-KDM1/LSD1 - Nuclear Marker (ab17721) specifically detects a band for KDM1/LSD1 (UniProt: O60341) at a molecular weight of 93kDa.
Trusted by the scientific community
Anti-KDM1/LSD1 - Nuclear Marker (ab17721) was first used in a scientific publication in 2005 and has been cited over 220 times in peer-reviewed journals.
Reviewed by scientists
Anti-KDM1/LSD1 - Nuclear Marker (ab17721) has over 25 independent reviews from customers.
Specificity confirmed
The specificity of Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721) has been confirmed by Western blot testing in KDM1A Knockout HAP1 cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: KDM1 / LSD1 knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: Jurkat whole cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab17721 observed at 110 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.ab17721 was shown to specifically react with KDMI/LSD1 in wild-type HAP1 cells. No band was observed when KDMI/LSD1 knockout samples were examined.
All lanes: Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
Predicted band size: 92 kDa
Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
ab17721 staining KDM1 / LSD1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab17721 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
KDM1/LSD1 Western blot staining using rabbit Anti-KDM1/LSD1 antibody
Observed MW: 110kd
Blocking Buffer & Diluting buffer concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721) at 1/1000 dilution
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg
Lane 2: HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates at 20 µg
Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 4: C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 5: Mouse skeletal muscle lysates at 20 µg
Lane 6: C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 92 kDa
Observed band size: 110 kDa
Exposure time: 50s
Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
All lanes: Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (Jurkat whole cell lysate ab7899) at 20 µg
Lane 3: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 92 kDa
Image courtesy of anonymous customer reviewerImmunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
ab17721 staining LSD1 in the mouse c2c12 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A diluted (1/2000) Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
This image is courtesy of Katia AncelinImmunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
Human 293T cells fixed in 4% paraformaldehyde were immunostained with ab17721 (1/200) for LSD1 and detected using FITC labelled anti-rabbit (Green). Nuclear DNA is stained blue with DAPI.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com