Rabbit Recombinant Monoclonal KDM2A antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.
Images
![Western blot - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (AB238945), expandable thumbnail](https://content.abcam.com/products/images/kdm2a-antibody-epr18602-bsa-and-azide-free-ab238945--western-blot-img138367.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (AB238945)")
![Immunocytochemistry/ Immunofluorescence - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (AB238945), expandable thumbnail](https://content.abcam.com/products/images/kdm2a-antibody-epr18602-bsa-and-azide-free-ab238945--immunocytochemistry-immunofluorescence-img14179.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (AB238945)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (AB238945), expandable thumbnail](https://content.abcam.com/products/images/kdm2a-antibody-epr18602-bsa-and-azide-free-ab238945--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24559.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (AB238945)")
![Immunoprecipitation - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (AB238945), expandable thumbnail](https://content.abcam.com/products/images/kdm2a-antibody-epr18602-bsa-and-azide-free-ab238945--immunoprecipitation-img70171.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (AB238945)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (AB238945), expandable thumbnail](https://content.abcam.com/products/images/kdm2a-antibody-epr18602-bsa-and-azide-free-ab238945--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81770.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (AB238945)")
Publications
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- Cell cycle (Georgetown, Tex.) 20:1935-19522021Up-regulation of miR-663a inhibits the cancer stem cell-like properties of glioma via repressing the KDM2A-mediated TGF-β/SMAD signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Wang,Bojuan Lang,Youdong Zhou,Jinyang Ma,Keqi HuPubMed 34424812
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Expected |
| Mouse | Tested | Expected | Expected | Expected |
| Rat | Expected | Expected | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
1 product for Alternative Product
Target data
Function
Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. Regulates circadian gene expression by repressing the transcriptional activator activity of CLOCK-BMAL1 heterodimer and RORA in a catalytically-independent manner (PubMed:26037310).
Alternative names
CXXC8, FBL11, FBL7, FBXL11, JHDM1A, KIAA1004, KDM2A, Lysine-specific demethylase 2A, CXXC-type zinc finger protein 8, F-box and leucine-rich repeat protein 11, F-box protein FBL7, F-box protein Lilina, F-box/LRR-repeat protein 11, JmjC domain-containing histone demethylation protein 1A, [Histone-H3]-lysine-36 demethylase 1A
Recommended products
Rabbit Recombinant Monoclonal KDM2A antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18602
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab238945 is the carrier-free version of Anti-KDM2A antibody [EPR18602] ab191387.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945), expandable thumbnail](https://content.abcam.com/products/images/kdm2a-antibody-epr18602-bsa-and-azide-free-ab238945--western-blot-img138367.jpg "Western blot - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)")
Western blot - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: KDM2A knockout HAP1 whole cell lysate (20 μg)
Lane 3: Hela whole cell lysate (20 μg)
Lane 4: Jurkat whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - Anti-KDM2A antibody [EPR18602] ab191387 observed at 133 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-KDM2A antibody [EPR18602] ab191387 was shown to specifically react with KDM2A in wild-type HAP1 cells. No band was observed when KDM2A knockout cells were examined. Wild-type and KDM2A knockout samples were subjected to SDS-PAGE. Anti-KDM2A antibody [EPR18602] ab191387 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1.313 ug/ml and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KDM2A antibody [EPR18602] ab191387).
All lanes: Western blot - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)
Predicted band size: 133 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945), expandable thumbnail](https://content.abcam.com/products/images/kdm2a-antibody-epr18602-bsa-and-azide-free-ab238945--immunocytochemistry-immunofluorescence-img14179.jpg "Immunocytochemistry/ Immunofluorescence - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)")
Immunocytochemistry/ Immunofluorescence - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling KDM2A with Anti-KDM2A antibody [EPR18602] ab191387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: Anti-KDM2A antibody [EPR18602] ab191387 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KDM2A antibody [EPR18602] ab191387).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945), expandable thumbnail](https://content.abcam.com/products/images/kdm2a-antibody-epr18602-bsa-and-azide-free-ab238945--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24559.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling KDM2A with Anti-KDM2A antibody [EPR18602] ab191387 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on epithelium cells of human colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KDM2A antibody [EPR18602] ab191387).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945), expandable thumbnail](https://content.abcam.com/products/images/kdm2a-antibody-epr18602-bsa-and-azide-free-ab238945--immunoprecipitation-img70171.jpg "Immunoprecipitation - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)")
Immunoprecipitation - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)
KDM2A was immunoprecipitated from 1mg of mouse brain whole cell lysate with Anti-KDM2A antibody [EPR18602] ab191387 at 1/100 dilution. Western blot was performed from the immunoprecipitate using Anti-KDM2A antibody [EPR18602] ab191387 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse brain whole cell lysate 10μg (Input).
Lane 2: Anti-KDM2A antibody [EPR18602] ab191387 IP in Mouse brain whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-KDM2A antibody [EPR18602] ab191387 in Mouse brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KDM2A antibody [EPR18602] ab191387).
All lanes: Immunoprecipitation - Anti-KDM2A antibody [EPR18602] (Anti-KDM2A antibody [EPR18602] ab191387)
Predicted band size: 133 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945), expandable thumbnail](https://content.abcam.com/products/images/kdm2a-antibody-epr18602-bsa-and-azide-free-ab238945--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81770.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling KDM2A with Anti-KDM2A antibody [EPR18602] ab191387 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on epithelium cells of mouse colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KDM2A antibody [EPR18602] ab191387).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945), expandable thumbnail](https://content.abcam.com/products/images/kdm2a-antibody-epr18602-bsa-and-azide-free-ab238945--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img31985.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling KDM2A with Anti-KDM2A antibody [EPR18602] ab191387 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on epithelium cells of rat colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KDM2A antibody [EPR18602] ab191387).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945), expandable thumbnail](https://content.abcam.com/products/images/kdm2a-antibody-epr18602-bsa-and-azide-free-ab238945--immunocytochemistry-immunofluorescence-img14423.jpg "Immunocytochemistry/ Immunofluorescence - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)")
Immunocytochemistry/ Immunofluorescence - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling KDM2A with Anti-KDM2A antibody [EPR18602] ab191387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on Jurkat cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: Anti-KDM2A antibody [EPR18602] ab191387 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KDM2A antibody [EPR18602] ab191387).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945), expandable thumbnail](https://content.abcam.com/products/images/kdm2a-antibody-epr18602-bsa-and-azide-free-ab238945--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img31985.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)")
![Immunocytochemistry/ Immunofluorescence - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945), expandable thumbnail](https://content.abcam.com/products/images/kdm2a-antibody-epr18602-bsa-and-azide-free-ab238945--immunocytochemistry-immunofluorescence-img14423.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-KDM2A antibody [EPR18602] - BSA and Azide free (ab238945)")
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