AB234082

Anti-KDM2B antibody [6F6G11]

Name: Anti-KDM2B antibody [6F6G11] (ab234082) | Abcam
Brand: Abcam Limited
SKU: AB234082
Price: 495 USD
Availability: InStock

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(2 Publications )

Mouse Monoclonal KDM2B antibody. Suitable for Flow Cyt, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KDM2B aa 450 to C-terminus.

4 Images

Flow Cytometry - Anti-KDM2B antibody [6F6G11] (AB234082)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-KDM2B antibody [6F6G11] (AB234082)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling KDM2B using ab234082 at 1/200 dilution (green) compared to a negative control (red).

Supplier Data

Western blot - Anti-KDM2B antibody [6F6G11] (AB234082)

All lanes:

Western blot - Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution

All lanes:

A549 (human lung carcinoma cell line) cell lysate

Predicted band size: 153 kDa

false

Supplier Data

Western blot - Anti-KDM2B antibody [6F6G11] (AB234082)

All lanes:

Western blot - Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

KDM2B (aa 457-555)-hIgGFc-transfected HEK-293 cell lysate

Predicted band size: 153 kDa

false

Supplier Data

Western blot - Anti-KDM2B antibody [6F6G11] (AB234082)

Expected recombinant MW : 36.8 kDa

All lanes:

Western blot - Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution

All lanes:

Human KDM2B (aa 457-555) recombinant protein

Predicted band size: 153 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6F6G11

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human KDM2B aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NHM5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code (PubMed : 16362057, PubMed : 17994099, PubMed : 26237645). Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36' (PubMed : 16362057, PubMed : 17994099, PubMed : 26237645). Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation (PubMed : 16362057, PubMed : 17994099). May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex (Probable).

See full target information KDM2B

Alternative Names

CXXC2, FBL10, FBXL10, JHDM1B, NDY1, PCCX2, KDM2B, Lysine-specific demethylase 2B, CXXC-type zinc finger protein 2, F-box and leucine-rich repeat protein 10, F-box protein FBL10, F-box/LRR-repeat protein 10, JmjC domain-containing histone demethylation protein 1B, Jumonji domain-containing EMSY-interactor methyltransferase motif protein, Protein-containing CXXC domain 2, [Histone-H3]-lysine-36 demethylase 1B, Protein JEMMA

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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

Bioengineering (Basel, Switzerland) 11: PubMed39329689

2024

Mesenchymal Stem Cell-Conditioned Media-Loaded Microparticles Enhance Acute Patency in Silk-Based Vascular Grafts.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine L Lorentz,Ande X Marini,Liza A Bruk,Prerak Gupta,Biman B Mandal,Morgan V DiLeo,Justin S Weinbaum,Steven R Little,David A Vorp

Nucleic acids research 49:4421-4440 PubMed33849069

2021

Bimodal regulation of the PRC2 complex by USP7 underlies tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dongxue Su,Wenjuan Wang,Yongqiang Hou,Liyong Wang,Xianfu Yi,Cheng Cao,Yuejiao Wang,Huan Gao,Yue Wang,Chao Yang,Beibei Liu,Xing Chen,Xiaodi Wu,Jiajing Wu,Dong Yan,Shuqi Wei,Lulu Han,Shumeng Liu,Qian Wang,Lei Shi,Lin Shan

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KDM2B antibody [6F6G11]

Flow Cytometry - Anti-KDM2B antibody [6F6G11] (AB234082)

Western blot - Anti-KDM2B antibody [6F6G11] (AB234082)

Western blot - Anti-KDM2B antibody [6F6G11] (AB234082)

Western blot - Anti-KDM2B antibody [6F6G11] (AB234082)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Alternative Names

Publications (2)

Product promise