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Rabbit Polyclonal KDM3A / JHDM2A antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications.


Images

Western blot - Anti-KDM3A / JHDM2A antibody (AB80598), expandable thumbnail
  • Immunoprecipitation - Anti-KDM3A / JHDM2A antibody (AB80598), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS

Form
Liquid
Clonality
Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Chimpanzee
Predicted
Predicted
Cow
Predicted
Predicted
Dog
Predicted
Predicted
Ferret
Predicted
Predicted
Gorilla
Predicted
Predicted
Pig
Predicted
Predicted
Rhesus monkey
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
2.00000-5.00000 µg/mg of lysate
Notes

-

Predicted
Predicted

Species
Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Cow, Dog
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000.00000 - 1/10000.00000
Notes

-

Predicted
Predicted

Species
Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Cow, Dog
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.

Alternative names

Recommended products

Rabbit Polyclonal KDM3A / JHDM2A antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique
Affinity purification Immunogen
Concentration
Loading...
Purification notes

Affinity purified using an epitope specific to KDM3A / JHDM2A immobilized on solid support.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-KDM3A / JHDM2A antibody (ab80598), expandable thumbnail

    Western blot - Anti-KDM3A / JHDM2A antibody (ab80598)

    All lanes: Western blot - Anti-KDM3A / JHDM2A antibody (ab80598) at 0.1 µg/mL

    Lane 1: whole HeLa cell lysate at 50 µg

    Lane 2: whole HeLa cell lysate at 15 µg

    Lane 3: whole HeLa cell lysate at 5 µg

    Predicted band size: 147 kDa

    Observed band size: 171 kDa, 300 kDa, 85 kDa

  • Immunoprecipitation - Anti-KDM3A / JHDM2A antibody (ab80598), expandable thumbnail

    Immunoprecipitation - Anti-KDM3A / JHDM2A antibody (ab80598)

    Detection of Human KDM3A / JHDM2A by Immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Lane 1: ab80598 used for IP at 3 μg/mg lysate; Lane 2: a control IgG was used for IP. For subsequent Western blot detection, ab80598 was used at 1 μg/ml.

    All lanes: Immunoprecipitation - Anti-KDM3A / JHDM2A antibody (ab80598)

    Predicted band size: 147 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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