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AB80598

Anti-KDM3A / JHDM2A antibody

3

(2 Reviews)

|

(5 Publications)

Rabbit Polyclonal KDM3A / JHDM2A antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

JHDM2A, JMJD1, JMJD1A, KIAA0742, TSGA, KDM3A, Lysine-specific demethylase 3A, JmjC domain-containing histone demethylation protein 2A, Jumonji domain-containing protein 1A, [histone H3]-dimethyl-L-lysine(9) demethylase 3A

2 Images
Immunoprecipitation - Anti-KDM3A / JHDM2A antibody (AB80598)
  • IP

Unknown

Immunoprecipitation - Anti-KDM3A / JHDM2A antibody (AB80598)

Detection of Human KDM3A / JHDM2A by Immunoprecipitation. Samples : Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies : Lane 1 : ab80598 used for IP at 3 μg/mg lysate; Lane 2 : a control IgG was used for IP. For subsequent Western blot detection, ab80598 was used at 1 μg/ml.

All lanes:

Immunoprecipitation - Anti-KDM3A / JHDM2A antibody (ab80598)

Predicted band size: 147 kDa

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Western blot - Anti-KDM3A / JHDM2A antibody (AB80598)
  • WB

Unknown

Western blot - Anti-KDM3A / JHDM2A antibody (AB80598)

All lanes:

Western blot - Anti-KDM3A / JHDM2A antibody (ab80598) at 0.1 µg/mL

Lane 1:

whole HeLa cell lysate at 50 µg

Lane 2:

whole HeLa cell lysate at 15 µg

Lane 3:

whole HeLa cell lysate at 5 µg

Predicted band size: 147 kDa

Observed band size: 171 kDa,300 kDa,85 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Affinity purified using an epitope specific to KDM3A / JHDM2A immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
See full target information KDM3A
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 31:345-359 PubMed37305393

2023

Mechanism of NURP1 in temozolomide resistance in hypoxia-treated glioma cells via the KDM3A/TFEB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Li,Xin Fu,Jie Wang,Wei Shang,Xiaotong Wang,Linyun Zhang,Jun Li

Journal of cellular and molecular medicine 25:8127-8139 PubMed34350711

2021

Overexpression of let-7d explains down-regulated KDM3A and ENO2 in the pathogenesis of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xu,Yonghui Song,Lili Lu

Cell reports 34:108574 PubMed33406418

2021

Zinc Finger Protein SALL4 Functions through an AT-Rich Motif to Regulate Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Nikki R Kong,Mahmoud A Bassal,Hong Kee Tan,Jesse V Kurland,Kol Jia Yong,John J Young,Yang Yang,Fudong Li,Jonathan D Lee,Yue Liu,Chan-Shuo Wu,Alicia Stein,Hongbo R Luo,Leslie E Silberstein,Martha L Bulyk,Daniel G Tenen,Li Chai

PloS one 12:e0188051 PubMed29145444

2017

Inhibition of the H3K9 methyltransferase G9A attenuates oncogenicity and activates the hypoxia signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jolene Caifeng Ho,Lissa Nurrul Abdullah,Qing You Pang,Sudhakar Jha,Edward Kai-Hua Chow,Henry Yang,Hiroyuki Kato,Lorenz Poellinger,Jun Ueda,Kian Leong Lee

The Journal of biological chemistry 288:16004-15 PubMed23546878

2013

Nitric oxide modifies global histone methylation by inhibiting Jumonji C domain-containing demethylases.

Applications

WB

Species

Human

Jason R Hickok,Divya Vasudevan,William E Antholine,Douglas D Thomas
View all publications
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