Rabbit Polyclonal KDM3A / JHDM2A antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications.
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
IP | WB | |
---|---|---|
Human | Tested | Tested |
Chimpanzee | Predicted | Predicted |
Cow | Predicted | Predicted |
Dog | Predicted | Predicted |
Ferret | Predicted | Predicted |
Gorilla | Predicted | Predicted |
Pig | Predicted | Predicted |
Rhesus monkey | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Cow, Dog | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Cow, Dog | Dilution info - | Notes - |
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Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
JHDM2A, JMJD1, JMJD1A, KIAA0742, TSGA, KDM3A, Lysine-specific demethylase 3A, JmjC domain-containing histone demethylation protein 2A, Jumonji domain-containing protein 1A, [histone H3]-dimethyl-L-lysine(9) demethylase 3A
Rabbit Polyclonal KDM3A / JHDM2A antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications.
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Affinity purified using an epitope specific to KDM3A / JHDM2A immobilized on solid support.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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All lanes: Western blot - Anti-KDM3A / JHDM2A antibody (ab80598) at 0.1 µg/mL
Lane 1: whole HeLa cell lysate at 50 µg
Lane 2: whole HeLa cell lysate at 15 µg
Lane 3: whole HeLa cell lysate at 5 µg
Predicted band size: 147 kDa
Observed band size: 171 kDa, 300 kDa, 85 kDa
Detection of Human KDM3A / JHDM2A by Immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Lane 1: ab80598 used for IP at 3 μg/mg lysate; Lane 2: a control IgG was used for IP. For subsequent Western blot detection, ab80598 was used at 1 μg/ml.
All lanes: Immunoprecipitation - Anti-KDM3A / JHDM2A antibody (ab80598)
Predicted band size: 147 kDa
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