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AB243641

Anti-KDM3A / JHDM2A antibody [EPR18875-106]

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(4 Publications)

Rabbit Recombinant Monoclonal KDM3A / JHDM2A antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

View Alternative Names

JHDM2A, JMJD1, JMJD1A, KIAA0742, TSGA, KDM3A, Lysine-specific demethylase 3A, JmjC domain-containing histone demethylation protein 2A, Jumonji domain-containing protein 1A, [histone H3]-dimethyl-L-lysine(9) demethylase 3A

10 Images
Immunocytochemistry/ Immunofluorescence - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling KDM3A / JHDM2A with ab243641 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line treatment with DFO (1mM) for 24h. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa cells treated with 1mM DFO for 24h (Red) / Untreated control (Green) labelling KDM3A / JHDM2A with ab243641 at 1/500 dilution (0.1ug) (Red) / (Green) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling KDM3A / JHDM2A with ab243641 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human testis (PMID : 25071150). The section was incubated with ab243641 for 30 mins at room temperature.T he immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunoprecipitation - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)
  • IP

Unknown

Immunoprecipitation - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)

KDM3A / JHDM2A was immunoprecipitated from 0.35 mg Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate with ab243641 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243641 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate 10 ug

Lane 2 : ab243641 IP in Ramos whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab243641 in Ramos whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (ab243641)

Predicted band size: 147 kDa

Observed band size: 147 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling KDM3A / JHDM2A with ab243641 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis (PMID : 17943087). The section was incubated with ab243641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling KDM3A / JHDM2A with ab243641 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat testis (PMID : 17943087). The section was incubated with ab243641 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)
  • WB

Lab

Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Fresh lysates were used in this WB.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (ab243641) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HeLa treated with 1mM Deferoxamine mesylate (DFO) for 24 hours, whole cell lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 147 kDa

Observed band size: 147 kDa

false

Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)
  • WB

Lab

Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (ab243641) at 1/1000 dilution

All lanes:

Ramos (human Burkitts lymphoma B lymphocyte), whole cell lysate at 30 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 147 kDa

Observed band size: 147 kDa

false

Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)
  • WB

Lab

Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

Fresh lysates were used in this WB.

The band at 60KD is the degragated fragment of KDM3A which is consistent with the literature(PMID : 31629659, 26728187).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (ab243641) at 1/1000 dilution

All lanes:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 147 kDa

Observed band size: 147 kDa,60 kDa

false

Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)
  • WB

Lab

Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

Fresh lysates were used in this WB.

The bands at 90, 60KD are the degragated fragments of KDM3A which is consistent with the literature (PMID : 31629659, 26728187).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (ab243641) at 1/1000 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 147 kDa

Observed band size: 147 kDa,60 kDa,90 kDa

false

  • Carrier free

    Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free

  • Carrier free

    Anti-KDM3A / JHDM2A antibody [EPR18875-106] - BSA and Azide free (Detector)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18875-106

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IP, IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

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Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
See full target information KDM3A
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

RNA biology 21:1-13 PubMed38131611

2023

LncRNA USP2-AS1 facilitates the osteogenic differentiation of bone marrow mesenchymal stem cells by targeting KDM3A/ETS1/USP2 to activate the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wanxin Luo,Na Zhang,Ziping Wang,Hao Chen,Jie Sun,Chen Yao,Yafeng Zhang

Bioengineered 13:13366-13383 PubMed36700466

2023

Role of sevoflurane in myocardial ischemia-reperfusion injury via the ubiquitin-specific protease 22/lysine-specific demethylase 3A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Song,Yang Wang,Hai-Yan Wang,Long-Long Guo

Molecular medicine reports 24: PubMed34080022

2021

SMARCC2 combined with c‑Myc inhibits the migration and invasion of glioma cells via modulation of the Wnt/β‑catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chiyang Li,Chengshuo Fei,Junjie Li,Hang Wu,Lei Chen,Ramzi Roshani,Hong Li,Linyong Shi,Chong Song,Junwei Gu,Yuntao Lu,Qiang Zhou

OncoTargets and therapy 13:11957-11973 PubMed33239895

2020

Histone Demethylase Promotes Cervical Cancer Malignancy Through the /KIF14/Hedgehog Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyu Liu,Dongqing Li,Xin Zhang,Yanyan Li,Jian Ou
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com