Skip to main content

Rabbit Recombinant Monoclonal KDM3B / JMJD1B antibody. Suitable for ICC/IF, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (AB271046), expandable thumbnail
  • Western blot - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (AB271046), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (AB271046), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (AB271046), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (AB271046), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Not recommended
Tested
Mouse
Tested
Not recommended
Tested
Tested
Tested
Rat
Expected
Not recommended
Tested
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

This antibody does not react with Human species for IHC.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/5000
Notes

This antibody does not react with Human species for IHC.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

This antibody does not react with Human species for IHC.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.

Alternative names

C5orf7, JHDM2B, JMJD1B, KIAA1082, KDM3B, Lysine-specific demethylase 3B, JmjC domain-containing histone demethylation protein 2B, Jumonji domain-containing protein 1B, Nuclear protein 5qNCA, [histone H3]-dimethyl-L-lysine(9) demethylase 3B

Recommended products

Rabbit Recombinant Monoclonal KDM3B / JMJD1B antibody. Suitable for ICC/IF, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24219-30
Purification technique
Affinity purification Protein A
Specificity

This antibody does not react with Human species for IHC.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

ab271046 does not react with Human species for IHC application.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046), expandable thumbnail

    Western blot - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lysates were made freshly and used in WB immediately to minimize protein degradation.

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046) at 1/1000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate at 20 µg

    Lane 2: 293T ( human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 191 kDa

    Observed band size: 220 kDa

  • Western blot - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046), expandable thumbnail

    Western blot - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lysates were made freshly and used in WB immediately to minimize protein degradation.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046) at 1/1000 dilution

    Lane 1: Mouse testis tissue lysate at 20 µg

    Lane 2: Mouse placenta tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 191 kDa

    Observed band size: 220 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling KDM3B / JMJD1B with ab271046 at 1/5000 dilution followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on rat testis. The section was incubated with ab271046 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling KDM3B / JMJD1B with ab271046 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubμue Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labeling KDM3B / JMJD1B with ab271046 at 1/500 dilution, followed by 150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling KDM3B / JMJD1B with ab271046 at 1/5000 dilution followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on mouse testis (PMID: 26681924). The section was incubated with ab271046 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry (Intracellular) - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling KDM3B / JMJD1B with ab271046 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-KDM3B / JMJD1B antibody [EPR24219-30] (ab271046)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling KDM3B / JMJD1B with ab271046 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com