Anti-KDM4A Antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-KDM4A Antibody (AB327187)

Immunofluorescent staining of human cell line U-2 OS using ab327187 at 0.25–2 µg/ml, showing positivity in nucleoli.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM4A Antibody (AB327187)

Immunohistochemical staining of human cerebellum using ab327187 at 1 : 200–1 : 500 dilution, showing strong positivity in nucleoli in Purkinje cells.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM4A Antibody (AB327187)

Immunohistochemical staining of human testis using ab327187 at 1 : 200–1 : 500 dilution, showing strong cytoplasmic positivity in cells in seminiferous ducts.

• ChIP

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ChIP - Anti-KDM4A Antibody (AB327187)

ChIP-Exo-Seq composite graph for ab327187 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

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Western blot - Anti-KDM4A Antibody (AB327187)

Western blot analysis in human cell line HEK 293 using ab327187 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-KDM4A Antibody (ab327187)

All lanes:

Human cell line HEK 293

Predicted band size: 120.66 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM4A Antibody (AB327187)

Immunohistochemical staining of human pancreas using ab327187 at 1 : 200–1 : 500 dilution, showing no positivity in exocrine glandular cells as expected.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM4A Antibody (AB327187)

Immunohistochemical staining of human skeletal muscle using ab327187 at 1 : 200–1 : 500 dilution, showing no positivity in myocytes as expected.