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Rabbit Recombinant Monoclonal KDM4B / JMJD2B antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Human samples.

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Images

Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (AB305057), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (AB305057), expandable thumbnail
  • Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (AB305057), expandable thumbnail
  • Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (AB305057), expandable thumbnail
  • Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (AB305057), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPIPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Not recommended
Not recommended
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate (PubMed:16603238, PubMed:28262558). Plays a critical role in the development of the central nervous system (CNS).

Alternative names

JHDM3B, JMJD2B, KIAA0876, KDM4B, Lysine-specific demethylase 4B, JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, [histone H3]-trimethyl-L-lysine(9) demethylase 4B

Recommended products

Rabbit Recombinant Monoclonal KDM4B / JMJD2B antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR25240-41
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (ab305057), expandable thumbnail

    Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (ab305057)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056).

    Western blot: Anti-KDM4B antibody [EPR25240-41] (Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056 was shown to bind specifically to KDM4B. A band was observed at 135 kDa in wild-type A549 cell lysates with no signal observed at this size in KDM4B knockout cell line. To generate this image, wild-type and KDM4B knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-41] (Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: KDM4B knockout A549 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 122 kDa

    Observed band size: 135 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (ab305057), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (ab305057)

    This data was developed using Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056, the same antibody clone in a different buffer formulation.
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (human colorectal adenocarcinoma epithelial cell) cells labeling KDM4B / JMJD2B with Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056 at 1/50 dilution (10.3 ug/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).

    Confocal image showing nuclear and weak cytoplasmic staining in SW480 cell line.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

  • Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (ab305057), expandable thumbnail

    Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (ab305057)

    This data was developed using Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056, the same antibody clone in a different buffer formulation.
    Buffer/dilution: 5% NFDM/TBST

    Exposure: 158 seconds

    Performed under reducing conditions.

    In Western blot, Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056 was shown to bind specifically to KDM4B/JMJD2B. A band was observed at 160 kDa in wild-type HAP1 cell lysates whereas no signal observed at this size in KDM4B/JMJD2B knockout cell line. 

    All lanes: Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-41] (Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: KDM4B / JMJD2B knockout HAP1 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 160 kDa

    Exposure time: 158s

  • Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (ab305057), expandable thumbnail

    Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (ab305057)

    This data was developed using Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling KDM4B / JMJD2B with Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056 at 1/200 dilution (2.575 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Positive staining in human breast.

    The section was incubated with Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (ab305057), expandable thumbnail

    Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (ab305057)

    This data was developed using Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling KDM4B / JMJD2B with Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056 at 1/200 dilution (2.575 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Positive staining in human breast carcinoma.

    The section was incubated with Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (ab305057), expandable thumbnail

    Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (ab305057)

    This data was developed using Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded cell pellets labeling KDM4B / JMJD2B with Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056 at 1/200 dilution (2.575 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
    Positive staining on (A) parental HAP1 cell pellet, (B) SW480 cell pellet, and (C) HEK-293 cell pellet, no staining on (D) KDM4B knockout HAP1 cell pellet.
    The section was incubated with Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (ab305057), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KDM4B / JMJD2B antibody [EPR25240-41] - BSA and Azide free (ab305057)

    This data was developed using Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056, the same antibody clone in a different buffer formulation.
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized wild-type HAP1 cells labeling KDM4B / JMJD2B with Anti-KDM4B / JMJD2B antibody [EPR25240-41] ab305056 at 1/50 dilution (10.3 ug/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).

    Confocal image showing nuclear staining in parental HAP1 cell line, and no staining in KDM4B KO HAP1 cell line.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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