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Anti-KDM4B / JMJD2B antibody [EPR25240-51] is a Rabbit Monoclonal antibody that is used in KDM4B / JMJD2B IHC-P, Western Blot. Suitable for Human samples.

KDM4B, also known as JMJD2B, is an enzyme that demethylates histone H3 at lysine 9 (H3K9), playing a pivotal role in altering the histone code. This enzyme is essential for chromatin remodeling and regulating gene expression, impacting critical processes like brain development and cancer progression.

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Images

Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (AB305058), expandable thumbnail
  • Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (AB305058), expandable thumbnail
  • Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (AB305058), expandable thumbnail
  • Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (AB305058), expandable thumbnail
  • Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (AB305058), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPIPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate (PubMed:16603238, PubMed:28262558). Plays a critical role in the development of the central nervous system (CNS).

Alternative names

JHDM3B, JMJD2B, KIAA0876, KDM4B, Lysine-specific demethylase 4B, JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, [histone H3]-trimethyl-L-lysine(9) demethylase 4B

Recommended products

Anti-KDM4B / JMJD2B antibody [EPR25240-51] is a Rabbit Monoclonal antibody that is used in KDM4B / JMJD2B IHC-P, Western Blot. Suitable for Human samples.

KDM4B, also known as JMJD2B, is an enzyme that demethylates histone H3 at lysine 9 (H3K9), playing a pivotal role in altering the histone code. This enzyme is essential for chromatin remodeling and regulating gene expression, impacting critical processes like brain development and cancer progression.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25240-51
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058), expandable thumbnail

    Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058)

    Blocking/dilution buffer: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    Lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058) at 1/1000 dilution

    All lanes: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 160 kDa

    Exposure time: 180s

  • Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058), expandable thumbnail

    Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058)

    Western blot: Anti-KDM4B antibody [EPR25240-51] (ab305058) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab305058 was shown to bind specifically to KDM4B. A band was observed at 135 kDa in wild-type A549 cell lysates with no signal observed at this size in KDM4B knockout cell line. To generate this image, wild-type and KDM4B knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: KDM4B knockout A549 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 122 kDa

    Observed band size: 135 kDa

  • Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058), expandable thumbnail

    Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058)

    Blocking/dilution buffer: 5% NFDM/TBST.

    This antibody does not cross-react with human JMJD2A/KDM4A, JMJD2C/KDM4C or JMJD2D/KDM4D.

    Exposure time: 10 seconds.

    All lanes: Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058) at 1/1000 dilution

    Lane 1: Flag-tagged human JMJD2A/KDM4A recombinant protein 10ng

    Lane 2: Flag-tagged human JMJD2B/KDM4B recombinant protein 10ng

    Lane 3: Flag-tagged human JMJD2C/KDM4C recombinant protein 10ng

    Lane 4: Flag-tagged human JMJD2D/KDM4D recombinant protein 10ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 160 kDa

    Exposure time: 10s

  • Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058), expandable thumbnail

    Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058)

    Immunohistochemical analysis of paraffin-embedded cell pellets labeling KDM4B / JMJD2B with ab305058 at 1/200 (2.3 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Positive staining on (A) parental HAP1 cell pellet, (B) SW480 cell pellet, and (C) HEK-293 cell pellet, no staining on (D) KDM4B knockout HAP1 cell pellet.

    The section was incubated with ab305058 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058), expandable thumbnail

    Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058)

    Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma tissue labeling KDM4B / JMJD2B with ab305058 at 1/200 (2.3 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human pancreatic carcinoma. The section was incubated with ab305058 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058), expandable thumbnail

    Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling KDM4B / JMJD2B with ab305058 at 1/200 (2.3 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human breast. The section was incubated with ab305058 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058), expandable thumbnail

    Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058)

    Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue labeling KDM4B / JMJD2B with ab305058 at 1/200 (2.3 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human endometrial carcinoma. The section was incubated with ab305058 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058), expandable thumbnail

    Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058)

    Blocking/dilution buffer: 5% NFDM/TBST.

    In Western blot, ab305058 was shown to bind specifically to KDM4B / JMJD2B. A band was observed at 160 kDa in wild-type HAP1 cell lysate whereas no signal observed at this size in KDM4B / JMJD2B knockout cell line.

    Lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    The bands between 50 kDa and 80 kDa are likely to be degraded target fragments.

    Exposure time: 158 seconds.

    All lanes: Western blot - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (ab305058) at 1/1000 dilution

    Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell line), whole cell lysate 20 µg

    Lane 2: KDM4B / JMJD2B knockout HAP1 whole cell lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Observed band size: 160 kDa

    Exposure time: 158s

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Product protocols

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