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AB313553

Anti-KDM4B / JMJD2B antibody [RP23040274]

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Rabbit Recombinant Multiclonal KDM4B / JMJD2B antibody. Suitable for ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KDM4B.

View Alternative Names

JHDM3B, JMJD2B, KIAA0876, KDM4B, Lysine-specific demethylase 4B, JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, [histone H3]-trimethyl-L-lysine(9) demethylase 4B

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-KDM4B / JMJD2B antibody [RP23040274] (AB313553)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KDM4B / JMJD2B antibody [RP23040274] (AB313553)

Immunofluorescent analysis of SK-OV-3 cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling JMJD2B with ab313553 at 1/100 dilution followed by Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of JMJD2B protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of JMJD2B. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ChIP - Anti-KDM4B / JMJD2B antibody [RP23040274] (AB313553)
  • ChIP

Supplier Data

ChIP - Anti-KDM4B / JMJD2B antibody [RP23040274] (AB313553)

Chromatin Immunoprecipitation (ChIP) assay of endogenous KDM4B protein using ab313553/ ChIP was performed using 5 µg ab313553 on sheared chromatin from 2 million PANC1 cells using the MAGnify ChIP System kit. Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to JUNB and ZEB1 transcriptional start site, PUMA intron 1 and SAT2 satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

Key facts

Host species

Rabbit

Clonality

Multiclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, ChIP

applications

Immunogen

Recombinant Fragment Protein within Human KDM4B.

O94953

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2.5 µg for 10^6 Cells", "ChIP-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: 99.91% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate (PubMed : 16603238, PubMed : 28262558). Plays a critical role in the development of the central nervous system (CNS).
See full target information KDM4B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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