AB93694

Anti-KDM4D / JMJD2D antibody

Name: Anti-KDM4D / JMJD2D antibody (ab93694) | Abcam
Brand: Abcam Limited
SKU: AB93694
Price: 530 USD
Availability: InStock

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(24 Publications)

Rabbit Polyclonal KDM4D / JMJD2D antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 24 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KDM4D aa 1-400.

View Alternative Names

JHDM3D, JMJD2D, KDM4D, Lysine-specific demethylase 4D, JmjC domain-containing histone demethylation protein 3D, Jumonji domain-containing protein 2D, [histone H3]-trimethyl-L-lysine(9) demethylase 4D

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody (AB93694)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody (AB93694)

ab93694, at 10μg/ml, staining KDM4D / JMJD2D in DLD1 (Human Colorectal Adenocarcinoma) cells by Immunofluorescence. Cells were fixed with 1% formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with normal goat serum. Secondary antibody : Goat anti Rabbit Cy3 at 1/250 dilution.

Unknown

Western blot - Anti-KDM4D / JMJD2D antibody (AB93694)

All lanes:

Western blot - Anti-KDM4D / JMJD2D antibody (ab93694) at 1/200 dilution

Lane 1:

DLD1 cell lysate at 10 µg

Lane 2:

DLD1 cell lysate at 5 µg

Predicted band size: 59 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human KDM4D aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

Q6B0I6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Product details

The immunogen used for this product shares 64%, 71% and 65% homology with KDM4A/JMJD2A, KDM4B/JMJD2B and KDM4C/JMJD2C respectively. Cross-reactivity with these proteins has not been confirmed experimentally.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Demethylates both di- and trimethylated H3 'Lys-9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.

See full target information KDM4D

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 20:e1012316 PubMed38905308

2024

Histone demethylase JMJD2D protects against enteric bacterial infection via up-regulating colonic IL-17F to induce β-defensin expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Zhang,Bei Li,Yilin Hong,Ping Luo,Zaifa Hong,Xiaochun Xia,Pingli Mo,Chundong Yu,Wenbo Chen

Frontiers in oncology 13:1295613 PubMed38045004

2023

Methylation of the epigenetic JMJD2D protein by SET7/9 promotes prostate tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruicai Gu,Tae-Dong Kim,Hanlin Jiang,Sook Shin,Sangphil Oh,Ralf Janknecht

JHEP reports : innovation in hepatology 5:100849 PubMed37701334

2023

JMJD2D stabilises and cooperates with HBx protein to promote HBV transcription and replication.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Kong,Zuofeng Liu,Ruyi Zhang,Fu'an Xie,Rubing Liang,Yong Zhang,Lingling Yu,Wensheng Yang,Xi Li,Qiang Chen,Bei Li,Yilin Hong,Ming Li,Xiaogang Xia,Lingwei Gu,Lijuan Fu,Xiaohua Li,Ye Shen,Ting Wu,Chundong Yu,Wengang Li

PloS one 18:e0281610 PubMed36780463

2023

Hippo signaling and histone methylation control cardiomyocyte cell cycle re-entry through distinct transcriptional pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhe Zhang,Miles Freeman,Yiqiang Zhang,Danny El-Nachef,George Davenport,Allison Williams,W Robb MacLellan

Oral diseases 29:2827-2836 PubMed36579641

2023

KDM4D enhances osteo/dentinogenic differentiation and migration of SCAPs via binding to RPS5.

Applications

Unspecified application

Species

Unspecified reactive species

Hanbing Liang,Qian Li,Ning Wang,Chunxiong Wang,Shaojing Shi,Haoqing Yang,Yangyang Cao,Ruitang Shi,Luyuan Jin,Chen Zhang

Proceedings of the National Academy of Sciences of the United States of America 119: PubMed35145029

2022

TRIM14 inhibits OPTN-mediated autophagic degradation of KDM4D to epigenetically regulate inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Di Liu,Zhiyao Zhao,Yuanchu She,Lei Zhang,Xiangtian Chen,Ling Ma,Jun Cui

Cell death discovery 7:284 PubMed34667158

2021

Histone demethylase KDM4D inhibition suppresses renal cancer progression and angiogenesis through JAG1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Yan,Liangsong Zhu,Jin Zhang,Zongming Lin

The EMBO journal 40:e107480 PubMed34269483

2021

Cytosolic GDH1 degradation restricts protein synthesis to sustain tumor cell survival following amino acid deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Jialiang Shao,Tiezhu Shi,Hua Yu,Yufeng Ding,Liping Li,Xiang Wang,Xiongjun Wang

The Journal of biological chemistry 296:100121 PubMed33434575

2021

Histone demethylase JMJD2D promotes the self-renewal of liver cancer stem-like cells by enhancing EpCAM and Sox9 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Deng,Ming Li,Minghui Zhuo,Peng Guo,Qiang Chen,Pingli Mo,Wengang Li,Chundong Yu

Theranostics 10:8863-8879 PubMed32754284

2020

Demethylase-independent function of JMJD2D as a novel antagonist of p53 to promote Liver Cancer initiation and progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Li,Yuan Deng,Minghui Zhuo,Hui Zhou,Xu Kong,Xiaogang Xia,Zhaojie Su,Qiang Chen,Peng Guo,Pingli Mo,Chundong Yu,Wengang Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KDM4D / JMJD2D antibody

Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody (AB93694)

Western blot - Anti-KDM4D / JMJD2D antibody (AB93694)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (24)

Product promise