AB93694

Anti-KDM4D / JMJD2D antibody

Name: Anti-KDM4D / JMJD2D antibody (ab93694) | Abcam
Brand: Abcam Limited
SKU: AB93694
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(20 Publications)

Rabbit Polyclonal KDM4D / JMJD2D antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 20 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KDM4D aa 1-400.

View Alternative Names

JHDM3D, JMJD2D, KDM4D, Lysine-specific demethylase 4D, JmjC domain-containing histone demethylation protein 3D, Jumonji domain-containing protein 2D, [histone H3]-trimethyl-L-lysine(9) demethylase 4D

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody (AB93694)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody (AB93694)

ab93694, at 10μg/ml, staining KDM4D / JMJD2D in DLD1 (Human Colorectal Adenocarcinoma) cells by Immunofluorescence. Cells were fixed with 1% formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with normal goat serum. Secondary antibody : Goat anti Rabbit Cy3 at 1/250 dilution.

Unknown

Western blot - Anti-KDM4D / JMJD2D antibody (AB93694)

All lanes:

Western blot - Anti-KDM4D / JMJD2D antibody (ab93694) at 1/200 dilution

Lane 1:

DLD1 cell lysate at 10 µg

Lane 2:

DLD1 cell lysate at 5 µg

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KDM4D aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

Q6B0I6

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

Product details

The immunogen used for this product shares 64%, 71% and 65% homology with KDM4A/JMJD2A, KDM4B/JMJD2B and KDM4C/JMJD2C respectively. Cross-reactivity with these proteins has not been confirmed experimentally.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Demethylates both di- and trimethylated H3 'Lys-9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.

See full target information KDM4D

style

left-column

Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 13:1295613 PubMed38045004

2023

Methylation of the epigenetic JMJD2D protein by SET7/9 promotes prostate tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruicai Gu,Tae-Dong Kim,Hanlin Jiang,Sook Shin,Sangphil Oh,Ralf Janknecht

JHEP reports : innovation in hepatology 5:100849 PubMed37701334

2023

JMJD2D stabilises and cooperates with HBx protein to promote HBV transcription and replication.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Kong,Zuofeng Liu,Ruyi Zhang,Fu'an Xie,Rubing Liang,Yong Zhang,Lingling Yu,Wensheng Yang,Xi Li,Qiang Chen,Bei Li,Yilin Hong,Ming Li,Xiaogang Xia,Lingwei Gu,Lijuan Fu,Xiaohua Li,Ye Shen,Ting Wu,Chundong Yu,Wengang Li

Oral diseases 29:2827-2836 PubMed36579641

2023

KDM4D enhances osteo/dentinogenic differentiation and migration of SCAPs via binding to RPS5.

Applications

Unspecified application

Species

Unspecified reactive species

Hanbing Liang,Qian Li,Ning Wang,Chunxiong Wang,Shaojing Shi,Haoqing Yang,Yangyang Cao,Ruitang Shi,Luyuan Jin,Chen Zhang

Proceedings of the National Academy of Sciences of the United States of America 119: PubMed35145029

2022

TRIM14 inhibits OPTN-mediated autophagic degradation of KDM4D to epigenetically regulate inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Di Liu,Zhiyao Zhao,Yuanchu She,Lei Zhang,Xiangtian Chen,Ling Ma,Jun Cui

Cell death discovery 7:284 PubMed34667158

2021

Histone demethylase KDM4D inhibition suppresses renal cancer progression and angiogenesis through JAG1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Yan,Liangsong Zhu,Jin Zhang,Zongming Lin

The Journal of biological chemistry 296:100121 PubMed33434575

2021

Histone demethylase JMJD2D promotes the self-renewal of liver cancer stem-like cells by enhancing EpCAM and Sox9 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Deng,Ming Li,Minghui Zhuo,Peng Guo,Qiang Chen,Pingli Mo,Wengang Li,Chundong Yu

Theranostics 10:8863-8879 PubMed32754284

2020

Demethylase-independent function of JMJD2D as a novel antagonist of p53 to promote Liver Cancer initiation and progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Li,Yuan Deng,Minghui Zhuo,Hui Zhou,Xu Kong,Xiaogang Xia,Zhaojie Su,Qiang Chen,Peng Guo,Pingli Mo,Chundong Yu,Wengang Li

Oncology research 28:161-175 PubMed31727206

2019

Enhancement of Radiosensitivity by Eurycomalactone in Human NSCLC Cells Through G₂/M Cell Cycle Arrest and Delayed DNA Double-Strand Break Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Nahathai Dukaew,Teruaki Konishi,Kongthawat Chairatvit,Narongchai Autsavapromporn,Noppamas Soonthornchareonnon,Ariyaphong Wongnoppavich

Cell metabolism 28:935-945.e5 PubMed30174306

2018

Short-Term Mitochondrial Permeability Transition Pore Opening Modulates Histone Lysine Methylation at the Early Phase of Somatic Cell Reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongfu Ying,Ge Xiang,Lingjun Zheng,Haite Tang,Lifan Duan,Xiaobing Lin,Qiuge Zhao,Keshi Chen,Yi Wu,Guangsuo Xing,Yiwang Lv,Linpeng Li,Liang Yang,Feixiang Bao,Qi Long,Yanshuang Zhou,Xueying He,Yaofeng Wang,Minghui Gao,Duanqing Pei,Wai-Yee Chan,Xingguo Liu

Molecular cancer 17:107 PubMed30060750

2018

Histone demethylase KDM4D promotes gastrointestinal stromal tumor progression through HIF1β/VEGFA signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Fuqing Hu,Haijie Li,Lu Liu,Feng Xu,Senyan Lai,Xuelai Luo,Junbo Hu,Xi Yang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KDM4D / JMJD2D antibody

Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody (AB93694)

Western blot - Anti-KDM4D / JMJD2D antibody (AB93694)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (20)

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise