Rabbit Recombinant Monoclonal KDM4D / JMJD2D antibody. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (AB289667), expandable thumbnail](https://content.abcam.com/products/images/kdm4d-jmjd2d-antibody-epr25241-24-ab289667--immunocytochemistry-immunofluorescence-img167240.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (AB289667)")
![Immunoprecipitation - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (AB289667), expandable thumbnail](https://content.abcam.com/products/images/kdm4d-jmjd2d-antibody-epr25241-24-ab289667--immunoprecipitation-img168187.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (AB289667)")
![Flow Cytometry (Intracellular) - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (AB289667), expandable thumbnail](https://content.abcam.com/products/images/kdm4d-jmjd2d-antibody-epr25241-24-ab289667--flow-cytometry-intracellular-img168930.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (AB289667)")
![Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (AB289667), expandable thumbnail](https://content.abcam.com/products/images/kdm4d-jmjd2d-antibody-epr25241-24-ab289667--western-blot-img173625.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (AB289667)")
![Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (AB289667), expandable thumbnail](https://content.abcam.com/products/images/kdm4d-jmjd2d-antibody-epr25241-24-ab289667--western-blot-img165024.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (AB289667)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | ChIP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ChIP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Demethylates both di- and trimethylated H3 'Lys-9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
Alternative names
JHDM3D, JMJD2D, KDM4D, Lysine-specific demethylase 4D, JmjC domain-containing histone demethylation protein 3D, Jumonji domain-containing protein 2D, [histone H3]-trimethyl-L-lysine(9) demethylase 4D
Recommended products
Rabbit Recombinant Monoclonal KDM4D / JMJD2D antibody. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR25241-24
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667), expandable thumbnail](https://content.abcam.com/products/images/kdm4d-jmjd2d-antibody-epr25241-24-ab289667--immunocytochemistry-immunofluorescence-img167240.jpg "Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)")
Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling KDM4D / JMJD2D with ab289667 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line. The nuclear counterstain is DAPI (Blue). Tubulin is labeled using Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution.
![Immunoprecipitation - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667), expandable thumbnail](https://content.abcam.com/products/images/kdm4d-jmjd2d-antibody-epr25241-24-ab289667--immunoprecipitation-img168187.jpg "Immunoprecipitation - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)")
Immunoprecipitation - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)
KDM4D / JMJD2D was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab289667 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab289667 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 μg (Input).
Lane 2: ab289667 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab289667 in HeLa whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)
Predicted band size: 59 kDa
Observed band size: 60 kDa
Exposure time: 10s
![Flow Cytometry (Intracellular) - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667), expandable thumbnail](https://content.abcam.com/products/images/kdm4d-jmjd2d-antibody-epr25241-24-ab289667--flow-cytometry-intracellular-img168930.jpg "Flow Cytometry (Intracellular) - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)")
Flow Cytometry (Intracellular) - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human cervix adenocarcinoma epithelial cell line) cells labeling KDM4D / JMJD2D with ab289667 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081)at 1/2000 dilution was used as the secondary antibody.
![Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667), expandable thumbnail](https://content.abcam.com/products/images/kdm4d-jmjd2d-antibody-epr25241-24-ab289667--western-blot-img173625.jpg "Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)")
Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)
Lysates were made freshly and used in the WB test immediately to minimize protein degradation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667) at 1/1000 dilution
All lanes: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 59 kDa
Observed band size: 60 kDa
Exposure time: 3min
![Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667), expandable thumbnail](https://content.abcam.com/products/images/kdm4d-jmjd2d-antibody-epr25241-24-ab289667--western-blot-img165024.jpg "Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)")
Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)
5% NFDM/TBST was used as a dilution and blocking buffer.
Low expression: ovary, spleen (PMID: 21293030).
All lanes: Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667) at 1/1000 dilution
Lane 1: Human testis tissue lysate at 20 µg
Lane 2: Human ovary tissue lysate at 20 µg
Lane 3: Human spleen tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 dilution
Predicted band size: 59 kDa
Observed band size: 60 kDa
Exposure time: 3min
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