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Rabbit Recombinant Monoclonal KDM4D / JMJD2D antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPChIPWBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Demethylates both di- and trimethylated H3 'Lys-9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.

Alternative names

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Rabbit Recombinant Monoclonal KDM4D / JMJD2D antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR25241-24

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab289673 is the carrier-free version of ab289667.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody [EPR25241-24] - BSA and Azide free (ab289673), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody [EPR25241-24] - BSA and Azide free (ab289673)

    This data was developed using ab289667, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling KDM4D / JMJD2D with ab289667 at 1/500 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line. The nuclear counterstain is DAPI (Blue). Tubulin is labeled using ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-KDM4D / JMJD2D antibody [EPR25241-24] - BSA and Azide free (ab289673), expandable thumbnail

    Immunoprecipitation - Anti-KDM4D / JMJD2D antibody [EPR25241-24] - BSA and Azide free (ab289673)

    This data was developed using ab289667, the same antibody clone in a different buffer formulation.

    KDM4D / JMJD2D was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab289667 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab289667 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input).
    Lane 2: ab289667 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289667 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (AB289667)

    Predicted band size: 59 kDa

    Observed band size: 60 kDa

    Exposure time: 10s

  • Flow Cytometry (Intracellular) - Anti-KDM4D / JMJD2D antibody [EPR25241-24] - BSA and Azide free (ab289673), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-KDM4D / JMJD2D antibody [EPR25241-24] - BSA and Azide free (ab289673)

    This data was developed using ab289667, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human cervix adenocarcinoma epithelial cell line) cells labeling KDM4D / JMJD2D with ab289667 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081)at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] - BSA and Azide free (ab289673), expandable thumbnail

    Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] - BSA and Azide free (ab289673)

    This data was developed using ab289667, the same antibody clone in a different buffer formulation.

    Lysates were made freshly and used in the WB test immediately to minimize protein degradation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (AB289667) at 1/1000 dilution

    All lanes: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Predicted band size: 59 kDa

    Observed band size: 60 kDa

    Exposure time: 3min

  • Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] - BSA and Azide free (ab289673), expandable thumbnail

    Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] - BSA and Azide free (ab289673)

    This data was developed using ab289667, the same antibody clone in a different buffer formulation.

    5% NFDM/TBST was used as a dilution and blocking buffer.

    Low expression: ovary, spleen (PMID: 21293030).

    All lanes: Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (AB289667) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 20 µg

    Lane 2: Human ovary tissue lysate at 20 µg

    Lane 3: Human spleen tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 dilution

    Predicted band size: 59 kDa

    Observed band size: 60 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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