AB70892

Anti-KDM5A / Jarid1A / RBBP2 antibody

Name: Anti-KDM5A / Jarid1A / RBBP2 antibody (ab70892) | Abcam
Brand: Abcam Limited
SKU: AB70892
Price: 525 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(26 Publications)

Rabbit Polyclonal KDM5A / Jarid1A / RBBP2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Human KDM5A aa 1600 to C-terminus.

View Alternative Names

JARID1A, RBBP2, RBP2, KDM5A, Lysine-specific demethylase 5A, Histone demethylase JARID1A, Jumonji/ARID domain-containing protein 1A, Retinoblastoma-binding protein 2, [histone H3]-trimethyl-L-lysine(4) demethylase 5A, RBBP-2

4 Images

Unknown

Immunoprecipitation - Anti-KDM5A / Jarid1A / RBBP2 antibody (AB70892)

HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded), with ab70892 at 1µg/ml for WB, after IP at 3 µg/mg lysate.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-KDM5A / Jarid1A / RBBP2 antibody (ab70892)

Predicted band size: 192 kDa

false

Supplier Data

Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody (AB70892)

All lanes:

Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody (ab70892) at 1/2000 dilution

Lane 1:

TCMK-1 (mouse kidney epithelial cell line) cell lysate

Lane 2:

Renca cell lysate

Predicted band size: 192 kDa

false

Unknown

Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody (AB70892)

All lanes:

Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody (ab70892) at 1/2000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Predicted band size: 192 kDa

false

Unknown

Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody (AB70892)

All lanes:

Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody (ab70892) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 192 kDa

Observed band size: 196 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human KDM5A aa 1600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P29375

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mL", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif (PubMed : 18270511). May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation (PubMed : 19430464). May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1 (By similarity). Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells (PubMed : 27427228).

See full target information KDM5A

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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 23:e3003362 PubMed40934285

2025

The mTORC2 subunit RICTOR drives breast cancer progression by promoting ganglioside biosynthesis through transcriptional and epigenetic mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Nafees Ansari,Somesh K Jha,Ali Khan,Kajal Rajput,Nishant Pandey,Dolly Jain,Rajeshwari Tripathi,Nihal Medatwal,Pankaj Sharma,Sudeshna Datta,Animesh Kar,Trishna Pani,Sk Asif Ali,Kaushavi Cholke,Kajal Rana,Valiya P Snijesh,Geetashree Mukherjee,Suryanarayana V S Deo,Soumen Basak,Ashutosh Mishra,Jyothi S Prabhu,Arnab Mukhopadhyay,Avinash Bajaj,Ujjaini Dasgupta

Cell reports 43:114991 PubMed39570746

2024

ZBTB7A is a modulator of KDM5-driven transcriptional networks in basal breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Benedetto DiCiaccio,Marco Seehawer,Zheqi Li,Andriana Patmanidis,Triet Bui,Pierre Foidart,Jun Nishida,Clive S D'Santos,Evangelia K Papachristou,Malvina Papanastasiou,Andrew H Reiter,Xintao Qiu,Rong Li,Yijia Jiang,Xiao-Yun Huang,Anton Simeonov,Stephen C Kales,Ganesha Rai,Madhu Lal-Nag,Ajit Jadhav,Myles Brown,Jason S Carroll,Henry W Long,Kornelia Polyak

Nature metabolism 6:1108-1127 PubMed38822028

2024

PNPO-PLP axis senses prolonged hypoxia in macrophages by regulating lysosomal activity.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Sekine,Haruna Takeda,Norihiko Takeda,Akihiro Kishino,Hayato Anzawa,Takayuki Isagawa,Nao Ohta,Shohei Murakami,Hideya Iwaki,Nobufumi Kato,Shu Kimura,Zun Liu,Koichiro Kato,Fumiki Katsuoka,Masayuki Yamamoto,Fumihito Miura,Takashi Ito,Masatomo Takahashi,Yoshihiro Izumi,Hiroyuki Fujita,Hitoshi Yamagata,Takeshi Bamba,Takaaki Akaike,Norio Suzuki,Kengo Kinoshita,Hozumi Motohashi

Cell death & disease 14:357 PubMed37301844

2023

The pediatric leukemia oncoprotein NUP98-KDM5A induces genomic instability that may facilitate malignant transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Joan Domingo-Reinés,Rosa Montes,Adrián Garcia-Moreno,Amador Gallardo,Jose Manuel Sanchez-Manas,Iván Ellson,Mar Lamolda,Chiara Calabro,Jose Antonio López-Escamez,Purificación Catalina,Pedro Carmona-Sáez,Pedro J Real,David Landeira,Verónica Ramos-Mejia

Nature genetics 54:1721-1735 PubMed36347944

2022

Histone H3 proline 16 hydroxylation regulates mammalian gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xijuan Liu,Jun Wang,Joshua A Boyer,Weida Gong,Shuai Zhao,Ling Xie,Qiong Wu,Cheng Zhang,Kanishk Jain,Yiran Guo,Javier Rodriguez,Mingjie Li,Hidetaka Uryu,Chengheng Liao,Lianxin Hu,Jin Zhou,Xiaobing Shi,Yi-Hsuan Tsai,Qin Yan,Weibo Luo,Xian Chen,Brian D Strahl,Alex von Kriegsheim,Qi Zhang,Gang Greg Wang,Albert S Baldwin,Qing Zhang

Cell biology and toxicology 39:1641-1655 PubMed36112263

2022

Fbxo22 inhibits metastasis in triple-negative breast cancer through ubiquitin modification of KDM5A and regulation of H3K4me3 demethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Siqiaozhi Li,Jinsong He,Xin Liao,Yixuan He,Rui Chen,Junhui Chen,Sean Hu,Jia Sun

Frontiers in cell and developmental biology 10:846092 PubMed35721502

2022

Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus.

Applications

Unspecified application

Species

Unspecified reactive species

Joan Domingo-Reinés,Gonzalo Martínez-Navajas,Rosa Montes,Mar Lamolda,Iris Simón,Julio Castaño,Rosa Ríos-Pelegrina,Javier Luis Lopez-Hidalgo,Raimundo García Del Moral,Juan A Marchal,Pedro J Real,Verónica Ramos-Mejía

Frontiers in oncology 11:654411 PubMed33842368

2021

miR-181d/RBP2/NF-κB p65 Feedback Regulation Promotes Chronic Myeloid Leukemia Blast Crisis.

Applications

Unspecified application

Species

Unspecified reactive species

Minran Zhou,Xiaolin Yin,Lixin Zheng,Yue Fu,Yue Wang,Zelong Cui,Zhenxing Gao,Xiaoming Wang,Tao Huang,Jihui Jia,Chunyan Chen

Journal of cellular and molecular medicine 25:4040-4052 PubMed33621431

2021

KDM5A silencing transcriptionally suppresses the FXYD3-PI3K/AKT axis to inhibit angiogenesis in hepatocellular cancer via miR-433 up-regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Shui Ma,Ting-Miao Wu,Bin Qian,Yu-Shan Liu,Hua Ding,Ming-Ming Fan,Ji-Bin Liu,Fei Yu,Hui-Min Wang,Yi Shi,Li-Peng Gu,Liu Li,Lin-Lin Tian,Pei-Yao Wang,Gao-Ren Wang,Zhi-Jun Wu,Qi-Fei Zou,Chang-Chun Ling,Da Fu

Frontiers in cell and developmental biology 8:581545 PubMed33553139

2021

Extracellular Vesicle-Encapsulated miR-29b-3p Released From Bone Marrow-Derived Mesenchymal Stem Cells Underpins Osteogenic Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Xueliang Zhang,Wenji Wang,Yongping Wang,Haiyan Zhao,Xingwen Han,Tong Zhao,Peng Qu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-KDM5A / Jarid1A / RBBP2 antibody

Immunoprecipitation - Anti-KDM5A / Jarid1A / RBBP2 antibody (AB70892)

Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody (AB70892)

Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody (AB70892)

Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody (AB70892)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (26)

Product promise