Rabbit Recombinant Monoclonal KDM5A / Jarid1A / RBBP2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (AB177486), expandable thumbnail](https://content.abcam.com/products/images/kdm5a-jarid1a-rbbp2-antibody-epr12742-ab177486--western-blot-img56800.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (AB177486)")
![Flow Cytometry (Intracellular) - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (AB177486), expandable thumbnail](https://content.abcam.com/products/images/kdm5a-jarid1a-rbbp2-antibody-epr12742-ab177486--flow-cytometry-intracellular-img2972.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (AB177486)")
![Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (AB177486), expandable thumbnail](https://content.abcam.com/products/images/kdm5a-jarid1a-rbbp2-antibody-epr12742-ab177486--western-blot-img116847.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (AB177486)")
Publications
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- BMC complementary and alternative medicine 18:1932018Dendrobium nobile Lindley and its bibenzyl component moscatilin are able to protect retinal cells from ischemia/hypoxia by dowregulating placental growth factor and upregulating Norrie disease protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Haur Chao et. al.PubMed 29933759
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Predicted | Not recommended | Not recommended | Predicted |
| Rat | Not recommended | Predicted | Not recommended | Not recommended | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif (PubMed:18270511). May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation (PubMed:19430464). May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1 (By similarity). Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells (PubMed:27427228).
Alternative names
JARID1A, RBBP2, RBP2, KDM5A, Lysine-specific demethylase 5A, Histone demethylase JARID1A, Jumonji/ARID domain-containing protein 1A, Retinoblastoma-binding protein 2, [histone H3]-trimethyl-L-lysine(4) demethylase 5A, RBBP-2
Recommended products
Rabbit Recombinant Monoclonal KDM5A / Jarid1A / RBBP2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12742
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486), expandable thumbnail](https://content.abcam.com/products/images/kdm5a-jarid1a-rbbp2-antibody-epr12742-ab177486--western-blot-img56800.jpg "Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)")
Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: KDM5A knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: HEK293 whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - ab177486 observed at 240 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab177486 was shown to specifically react with KDM5A in wild-type HAP1 cells. No bands were observed when KDM5A knockout samples were used. Wild-type and KDM5A knockout samples were subjected to SDS-PAGE. ab177486 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)
Predicted band size: 192 kDa
![Flow Cytometry (Intracellular) - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486), expandable thumbnail](https://content.abcam.com/products/images/kdm5a-jarid1a-rbbp2-antibody-epr12742-ab177486--flow-cytometry-intracellular-img2972.jpg "Flow Cytometry (Intracellular) - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)")
Flow Cytometry (Intracellular) - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)
Intracellular flow cytometric analysis of permeabilized NCCIT cells labeling KDM5A / Jarid1A / RBBP2 with ab177486 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
![Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486), expandable thumbnail](https://content.abcam.com/products/images/kdm5a-jarid1a-rbbp2-antibody-epr12742-ab177486--western-blot-img116847.jpg "Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)")
Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)
All lanes: Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: MCF7 cell lysate at 10 µg
Lane 4: NCCIT cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 192 kDa
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