Mouse Monoclonal KDM5B / PLU1 / Jarid1B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KDM5B aa 1400-1500.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunocytochemistry-immunofluorescence-img8849.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62541.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102879.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120712.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59978.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)")
Publications
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- Veterinary and comparative oncology 19:518-5282021JARID1-targeted histone H3 demethylase inhibitors exhibit anti-proliferative activity and overcome cisplatin resistance in canine oral melanoma cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSavannah J Tobin,Hong Chang,Michael S Kent,Alexander E DaviesPubMed 33715247
- Cancer management and research 12:9197-92092020microRNA-139-3p Inhibits Malignant Behaviors of Laryngeal Cancer Cells via the KDM5B/SOX2 Axis and the Wnt/β-Catenin Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifei Ma et. al.PubMed 33061611
- Molecular therapy. Nucleic acids 22:846-8612020MicroRNA let-7i Inhibits Histone Lysine Demethylase KDM5B to Halt Esophageal Cancer Progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Yang et. al.PubMed 33230480
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human KDM5B aa 1400-1500. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UGL1
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code (PubMed:24952722, PubMed:27214403, PubMed:28262558). Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5 (PubMed:24952722). In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity).
Alternative names
JARID1B, PLU1, RBBP2H1, KDM5B, Lysine-specific demethylase 5B, Cancer/testis antigen 31, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Retinoblastoma-binding protein 2 homolog 1, [histone H3]-trimethyl-L-lysine(4) demethylase 5B, CT31, RBP2-H1
Recommended products
Mouse Monoclonal KDM5B / PLU1 / Jarid1B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KDM5B aa 1400-1500.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human KDM5B aa 1400-1500. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UGL1
- Clone number
- CL1129
- Purification technique
- Affinity purification Protein A
- Epitope
- Binds to an epitope located within the peptidesequence DGINSLERKLKRRLE as determined by overlapping synthetic peptides.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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9 product images
![Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunocytochemistry-immunofluorescence-img8849.jpg "Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)")
Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)
Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labeling KDM5B using ab24420 at 10 μg/ml showing specific staining in the nucleoplasm in green. Microtubules are visualized in red.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62541.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)
Paraffin-embedded human uterus tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102879.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)
Paraffin-embedded human prostate tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120712.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)
Paraffin-embedded human prostate cancer tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59978.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)
Paraffin-embedded human colorectal cancer tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104000.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)
Paraffin-embedded human stomach cancer tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57817.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)
Paraffin-embedded human breast cancer tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.
![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--western-blot-img117940.jpg "Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)")
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)
All lanes: Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220) at 1 µg
Lane 1: Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2: KDM5B / PLU1 / Jarid1B overexpression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Predicted band size: 175 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78547.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)
Paraffin-embedded human liver tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis. Negative control.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104000.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57817.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)")
![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--western-blot-img117940.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220), expandable thumbnail](https://content.abcam.com/products/images/kdm5b-plu1-jarid1b-antibody-cl1129-ab244220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78547.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)")
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