AB244220

Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129]

Name: Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220) | Abcam
Brand: Abcam Limited
SKU: AB244220
Price: 630 USD
Availability: InStock

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(7 Publications)

Mouse Monoclonal KDM5B / PLU1 / Jarid1B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KDM5B aa 1400-1500.

View Alternative Names

JARID1B, PLU1, RBBP2H1, KDM5B, Lysine-specific demethylase 5B, Cancer/testis antigen 31, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Retinoblastoma-binding protein 2 homolog 1, [histone H3]-trimethyl-L-lysine(4) demethylase 5B, CT31, RBP2-H1

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Paraffin-embedded human prostate tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Paraffin-embedded human prostate cancer tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Paraffin-embedded human uterus tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Paraffin-embedded human colorectal cancer tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labeling KDM5B using ab24420 at 10 μg/ml showing specific staining in the nucleoplasm in green. Microtubules are visualized in red.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Paraffin-embedded human breast cancer tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Paraffin-embedded human stomach cancer tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Paraffin-embedded human liver tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis. Negative control.

Supplier Data

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

All lanes:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220) at 1 µg

Lane 1:

Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

KDM5B / PLU1 / Jarid1B overexpression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

Predicted band size: 175 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL1129

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KDM5B aa 1400-1500. The exact immunogen used to generate this antibody is proprietary information.

Q9UGL1

Epitope

Binds to an epitope located within the peptidesequence DGINSLERKLKRRLE as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code (PubMed : 24952722, PubMed : 27214403, PubMed : 28262558). Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5 (PubMed : 24952722). In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity).

See full target information KDM5B

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature neuroscience 28:1598-1609 PubMed40659844

2025

An expanded subventricular zone supports postnatal cortical interneuron migration in gyrencephalic brains.

Applications

Unspecified application

Species

Unspecified reactive species

JaeYeon Kim,Aunoy Poddar,Kadellyn Sandoval,Julia Chu,Emma Horton,Di Cui,Keira Nakamura,I-Ling Lu,Michael Mui,Theresa Bartels,Christian M Wood,Susana I Ramos,David H Rowitch,Nadejda M Tsankova,Hosung Kim,Chet C Sherwood,Boris W Kramer,Angela C Roberts,Pablo J Ross,Duan Xu,Nicola J Robertson,Elizabeth A Maga,Peng Ji,Mercedes F Paredes

International journal of medical sciences 21:2158-2169 PubMed39239542

2024

Multiple Comprehensive Analyses Identify Lysine Demethylase KDM as a Potential Therapeutic Target for Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wan-Jou Shen,Hsuan-Min Kao,Chih-Yang Wang,Rubina Kousar,Jing-Shan Lin,Ching-Chung Ko,Hung-Yun Lin,Hoang Dang Khoa Ta,Gangga Anuraga,Do Thi Minh Xuan,Sachin Kumar,Sanskriti Dey,Ngoc Phung Ly,Wei-Jan Wang

British journal of cancer 131:271-282 PubMed38862740

2024

Tumour cell-derived serglycin promotes IL-8 secretion of CAFs in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Li,Guiping Xie,Jia Chen,Yaohui Wang,Jing Zhai,Lizong Shen

Journal of cancer research and clinical oncology 150:294 PubMed38842683

2024

LncRNA HOXC-AS3 accelerates malignant proliferation of cervical cancer cells via stabilizing KDM5B.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Fang Hou,Zhenghua Teng,Weiwei Xia,Jie Peng

Veterinary and comparative oncology 19:518-528 PubMed33715247

2021

JARID1-targeted histone H3 demethylase inhibitors exhibit anti-proliferative activity and overcome cisplatin resistance in canine oral melanoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Savannah J Tobin,Hong Chang,Michael S Kent,Alexander E Davies

Cancer management and research 12:9197-9209 PubMed33061611

2020

microRNA-139-3p Inhibits Malignant Behaviors of Laryngeal Cancer Cells via the KDM5B/SOX2 Axis and the Wnt/β-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yifei Ma,Zili Chen,Guodong Yu

Molecular therapy. Nucleic acids 22:846-861 PubMed33230480

2020

MicroRNA let-7i Inhibits Histone Lysine Demethylase KDM5B to Halt Esophageal Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Wenhua Li,Bochong Wei,Kai Wu,Donglei Liu,Dengyan Zhu,Chunyang Zhang,Fengbiao Wen,Yuxia Fan,Song Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (AB244220)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

Product promise