AB181089

Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704]

Name: Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) | Abcam
Brand: Abcam Limited
SKU: AB181089
Price: 395 USD
Availability: InStock
Rating: 5 (3 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(3 Reviews)

(28 Publications)

Rabbit Recombinant Monoclonal KDM5B / PLU1 / Jarid1B antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 28 publications.

View Alternative Names

JARID1B, PLU1, RBBP2H1, KDM5B, Lysine-specific demethylase 5B, Cancer/testis antigen 31, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Retinoblastoma-binding protein 2 homolog 1, [histone H3]-trimethyl-L-lysine(4) demethylase 5B, CT31, RBP2-H1

5 Images

Lab

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (AB181089)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : KDM5B knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)

Lanes 1 - 3 : Merged signal (red and green). Green - ab181089 observed at 170 kDa. Red - loading control, ab18058, observed at 120 kDa.

ab181089 was shown to recognize KDM5B in wild-type HAP1 cells as signal was lost at the expected MW in KDM5B knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and KDM5B knockout samples were subjected to SDS-PAGE. ab181089 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1.9 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)

Predicted band size: 175 kDa

false

Supplier Data

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (AB181089)

All lanes:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/1000 dilution

All lanes:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 175 kDa

Observed band size: 170 kDa

false

Lab

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (AB181089)

Blocking and dilution buffer : 5% NFDM/TBST.

Lane 1:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/1000 dilution

Lane 1:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/kdm5b-plu1-jarid1b-antibody-epr12704-bsa-and-azide-free-ab232590'>ab232590</a>) at 1/1000 dilution

All lanes:

T47-D (human ductal carcinoma epithelia) whole cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG H&L at 1/1000 dilution

Predicted band size: 175 kDa

Observed band size: 170 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (AB181089)

Lanes 1 - 2:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/5000 dilution

Lanes 1 - 2:

Lane 1:

BxPC-3 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 175 kDa

Observed band size: 170 kDa

false

Lab

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (AB181089)

Blocking and dilution buffer : 5% NFDM/TBST.

Lanes 1 - 2:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/5000 dilution

Lanes 1 - 2:

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG H&L at 1/1000 dilution

Predicted band size: 175 kDa

Observed band size: 170 kDa

false

Exposure time: 3min

Carrier free

Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12704

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code (PubMed : 24952722, PubMed : 27214403, PubMed : 28262558). Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5 (PubMed : 24952722). In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity).

See full target information KDM5B

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 17:1459358 PubMed39664113

2024

Effect of histone demethylase KDM5B on long-term cognitive impairment in neonatal rats induced by sevoflurane.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhong Wang,Yun Chen,Mengxiao Zhang,Chengdong Yuan,Yu Zhang,Xingjian Liu,Yi Zhang,Xiaoli Liang

Journal of cellular and molecular medicine 28:e70237 PubMed39643939

2024

Endothelial KDM5B Regulated by Piezo1 Contributes to Disturbed Flow Induced Atherosclerotic Plaque Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Wu,Shanshan Jiang,Xiao Zhou,Wei Li,Jiaqi Ke,Ziting Liu,Lijie Ren,Qiongyu Lu,Fengchan Li,Chaojun Tang,Li Zhu

Nature cell biology 26:1745-1758 PubMed39169219

2024

RNA sequestration in P-bodies sustains myeloid leukaemia.

Applications

Unspecified application

Species

Unspecified reactive species

Srikanth Kodali,Ludovica Proietti,Gemma Valcarcel,Anna V López-Rubio,Patrizia Pessina,Thomas Eder,Junchao Shi,Annie Jen,Núria Lupión-Garcia,Anne C Starner,Mason D Bartels,Yingzhi Cui,Caroline M Sands,Ainoa Planas-Riverola,Alba Martínez,Talia Velasco-Hernandez,Laureano Tomás-Daza,Bernhard Alber,Gabriele Manhart,Isabella Maria Mayer,Karoline Kollmann,Alessandro Fatica,Pablo Menendez,Evgenia Shishkova,Rachel E Rau,Biola M Javierre,Joshua Coon,Qi Chen,Eric L Van Nostrand,Jose L Sardina,Florian Grebien,Bruno Di Stefano

Journal of cancer research and clinical oncology 150:294 PubMed38842683

2024

LncRNA HOXC-AS3 accelerates malignant proliferation of cervical cancer cells via stabilizing KDM5B.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Fang Hou,Zhenghua Teng,Weiwei Xia,Jie Peng

Cell death discovery 10:252 PubMed38789418

2024

KDM5B promotes SMAD4 loss-driven drug resistance through activating DLG1/YAP to induce lipid accumulation in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yumin Wang,Shiqian Liu,Yan Wang,Baibei Li,Jiaming Liang,Yu Chen,Bo Tang,Shuiping Yu,Hongquan Wang

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38575342

2024

The Intellectual Disability Risk Gene Regulates Long-Term Memory Consolidation in the Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Leticia Pérez-Sisqués,Shail U Bhatt,Rugile Matuleviciute,Talia E Gileadi,Eniko Kramar,Andrew Graham,Franklin G Garcia,Ashley Keiser,Dina P Matheos,James A Cain,Alan M Pittman,Laura C Andreae,Cathy Fernandes,Marcelo A Wood,K Peter Giese,M Albert Basson

Nature 626:881-890 PubMed38297124

2024

An epigenetic barrier sets the timing of human neuronal maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriele Ciceri,Arianna Baggiolini,Hyein S Cho,Meghana Kshirsagar,Silvia Benito-Kwiecinski,Ryan M Walsh,Kelly A Aromolaran,Alberto J Gonzalez-Hernandez,Hermany Munguba,So Yeon Koo,Nan Xu,Kaylin J Sevilla,Peter A Goldstein,Joshua Levitz,Christina S Leslie,Richard P Koche,Lorenz Studer

Technology in cancer research & treatment 22:15330338231175768 PubMed37254514

2023

PLU1 Promotes the Proliferation and Migration of Glioma Cells and Regulates Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Wentao Dong,Lude Wang,Yi Pan,Qi Tu,Ting He,Tian Zhou,Jianlie Yuan,Minfeng Tong

Nature 617:386-394 PubMed37100912

2023

A druggable copper-signalling pathway that drives inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Stéphanie Solier,Sebastian Müller,Tatiana Cañeque,Antoine Versini,Arnaud Mansart,Fabien Sindikubwabo,Leeroy Baron,Laila Emam,Pierre Gestraud,G Dan Pantoș,Vincent Gandon,Christine Gaillet,Ting-Di Wu,Florent Dingli,Damarys Loew,Sylvain Baulande,Sylvère Durand,Valentin Sencio,Cyril Robil,François Trottein,David Péricat,Emmanuelle Näser,Céline Cougoule,Etienne Meunier,Anne-Laure Bègue,Hélène Salmon,Nicolas Manel,Alain Puisieux,Sarah Watson,Mark A Dawson,Nicolas Servant,Guido Kroemer,Djillali Annane,Raphaël Rodriguez

Current issues in molecular biology 45:3219-3237 PubMed37185734

2023

Knockdown of KDM5B Leads to DNA Damage and Cell Cycle Arrest in Granulosa Cells via MTF1.

Applications

Unspecified application

Species

Unspecified reactive species

Yingnan Yang,Yu Cai,Jinjing Guo,Keke Dai,Liang Liu,Zili Chen,Feng Wang,Mingtian Deng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704]

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (AB181089)

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (AB181089)

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (AB181089)

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (AB181089)

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (AB181089)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (28)

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