AB232590

Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal KDM5B / PLU1 / Jarid1B antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

JARID1B, PLU1, RBBP2H1, KDM5B, Lysine-specific demethylase 5B, Cancer/testis antigen 31, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Retinoblastoma-binding protein 2 homolog 1, [histone H3]-trimethyl-L-lysine(4) demethylase 5B, CT31, RBP2-H1

5 Images

Lab

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (AB232590)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : KDM5B knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)

Lanes 1 - 3 : Merged signal (red and green). Green - ab181089 observed at 170 kDa. Red - loading control, ab18058, observed at 120 kDa.

ab181089 was shown to recognize KDM5B in wild-type HAP1 cells as signal was lost at the expected MW in KDM5B knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and KDM5B knockout samples were subjected to SDS-PAGE. ab181089 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1.9 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181089).

All lanes:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (<a href='/en-us/products/primary-antibodies/kdm5b-plu1-jarid1b-antibody-epr12704-ab181089'>ab181089</a>)

Predicted band size: 175 kDa

false

Supplier Data

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (AB232590)

All lanes:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (<a href='/en-us/products/primary-antibodies/kdm5b-plu1-jarid1b-antibody-epr12704-ab181089'>ab181089</a>) at 1/1000 dilution

All lanes:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 175 kDa

Observed band size: 170 kDa

false

Lab

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (AB232590)

Lane 1:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (<a href='/en-us/products/primary-antibodies/kdm5b-plu1-jarid1b-antibody-epr12704-ab181089'>ab181089</a>) at 1/1000 dilution

Lane 1:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590) at 1/1000 dilution

All lanes:

T47-D (human ductal carcinoma epithelia) whole cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG H&L at 1/1000 dilution

Predicted band size: 175 kDa

Observed band size: 170 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (AB232590)

Lanes 1 - 2:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (<a href='/en-us/products/primary-antibodies/kdm5b-plu1-jarid1b-antibody-epr12704-ab181089'>ab181089</a>) at 1/5000 dilution

Lanes 1 - 2:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590) at 1/5000 dilution

Lane 1:

BxPC-3 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 175 kDa

Observed band size: 170 kDa

false

Lab

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (AB232590)

Lanes 1 - 2:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (<a href='/en-us/products/primary-antibodies/kdm5b-plu1-jarid1b-antibody-epr12704-ab181089'>ab181089</a>) at 1/5000 dilution

Lanes 1 - 2:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590) at 1/5000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG H&L at 1/1000 dilution

Predicted band size: 175 kDa

Observed band size: 170 kDa

false

Exposure time: 3min

Unconjugated

Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12704

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab232590 is the carrier-free version of ab181089.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code (PubMed : 24952722, PubMed : 27214403, PubMed : 28262558). Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5 (PubMed : 24952722). In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity).

See full target information KDM5B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 7:493-507 PubMed39972217

2025

Pro-inflammatory macrophages produce mitochondria-derived superoxide by reverse electron transport at complex I that regulates IL-1β release during NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Alva M Casey,Dylan G Ryan,Hiran A Prag,Suvagata Roy Chowdhury,Eloïse Marques,Keira Turner,Anja V Gruszczyk,Ming Yang,Dane M Wolf,Jan Lj Miljkovic,Joyce Valadares,Patrick F Chinnery,Richard C Hartley,Christian Frezza,Julien Prudent,Michael P Murphy

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (AB232590)

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (AB232590)

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (AB232590)

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (AB232590)

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (AB232590)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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