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AB325403

Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] - BSA and Azide free

  • RabMAb
  • Recombinant
  • KO Validated
  • Advanced Validation
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Rabbit Recombinant Monoclonal KDM5B / PLU1 / Jarid1B antibody. Carrier free. Suitable for IP, WB, ChIC/CUT&RUN-seq and reacts with Human samples.

View Alternative Names

JARID1B, PLU1, RBBP2H1, KDM5B, Lysine-specific demethylase 5B, Cancer/testis antigen 31, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Retinoblastoma-binding protein 2 homolog 1, [histone H3]-trimethyl-L-lysine(4) demethylase 5B, CT31, RBP2-H1

7 Images
Immunoprecipitation - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] - BSA and Azide free (AB325403)
  • IP

Supplier Data

Immunoprecipitation - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] - BSA and Azide free (AB325403)

This data was developed using ab325395, the same antibody clone in a different buffer formulation.

KDM5B / PLU1 / Jarid1B was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab325395 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325395 at 1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] (<a href='/en-us/products/primary-antibodies/kdm5b-plu1-jarid1b-antibody-epr30394-502-ab325395'>ab325395</a>) at 1/30 dilution

Lanes 1 - 2:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/kdm5b-plu1-jarid1b-antibody-epr30394-502-ab325395'>ab325395</a> in K-562 lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 180 kDa

false

Exposure time: 58s

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] - BSA and Azide free (AB325403)
  • WB

Supplier Data

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] - BSA and Azide free (AB325403)

This data was developed using ab325395, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab325395 was shown to bind specifically to KDM5B. Target of interest was observed at 180 kDa in wild-type HAP1 cell lysates (lane 1) with no signal observed at this size in KDM5B knockout cell line (lane 2) .

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] (<a href='/en-us/products/primary-antibodies/kdm5b-plu1-jarid1b-antibody-epr30394-502-ab325395'>ab325395</a>) at 1/1000 dilution

Lane 1:

Wilde-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

Lane 2:

KDM5B knockout HAP1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 180 kDa,36 kDa

false

Exposure time: 10s

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] - BSA and Azide free (AB325403)
  • WB

Supplier Data

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] - BSA and Azide free (AB325403)

This data was developed using ab325395, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] (<a href='/en-us/products/primary-antibodies/kdm5b-plu1-jarid1b-antibody-epr30394-502-ab325395'>ab325395</a>) at 1/1000 dilution

All lanes:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 180 kDa,36 kDa

false

Exposure time: 10s

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] - BSA and Azide free (AB325403)
  • WB

Supplier Data

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] - BSA and Azide free (AB325403)

This data was developed using ab325395, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab325395 was shown to bind specifically to KDM5B. Target of interest was observed at 180 kDa in wild-type HCT 116 cell lysates (lane 1) with no signal observed at this size in KDM5B knockout cell line (lane 2) (lane 2, knockout cell line ab287592).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] (<a href='/en-us/products/primary-antibodies/kdm5b-plu1-jarid1b-antibody-epr30394-502-ab325395'>ab325395</a>) at 1/1000 dilution

Lane 2:

HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

KDM5B knockout HCT 116 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 180 kDa,36 kDa

false

Exposure time: 180s

ChIC/CUT&RUN sequencing - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] - BSA and Azide free (AB325403)
  • ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] - BSA and Azide free (AB325403)

This data was developed using ab325395, the same antibody clone in a different buffer formulation.

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 K-562 (human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab325395 [EPR30394-502]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

ChIC/CUT&RUN sequencing - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] - BSA and Azide free (AB325403)
  • ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] - BSA and Azide free (AB325403)

This data was developed using ab325395, the same antibody clone in a different buffer formulation.

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 K-562 (human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab325395 [EPR30394-502]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

ChIC/CUT&RUN sequencing - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] - BSA and Azide free (AB325403)
  • ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502] - BSA and Azide free (AB325403)

This data was developed using ab325395, the same antibody clone in a different buffer formulation.

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 K-562 (human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab325395 [EPR30394-502]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

  • Unconjugated

    Anti-KDM5B / PLU1 / Jarid1B antibody [EPR30394-502]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30394-502

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ChIC/CUT&RUN-seq, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ChIPseq-species-checked": "notRecommended", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "<p></p>" } } }
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Product details

ab325403 is the carrier-free version of ab325395.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code (PubMed : 24952722, PubMed : 27214403, PubMed : 28262558). Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5 (PubMed : 24952722). In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity).
See full target information KDM5B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com