Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18]

• WB

Supplier Data

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (AB259913)

Blocking and Diluting buffer and concentration : Intercept^® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

Exposure time 70 seconds

Smaller bands are non-specific signal.

All lanes:

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913) at 1/1000 dilution

Lane 1:

HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate at 20 µg

Lane 2:

HEK293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 175 kDa

Observed band size: 180 kDa

false

• WB

Supplier Data

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (AB259913)

Blocking and Diluting buffer and concentration : 5% NFDM/TBST

Exposure time 3 minutes

Smaller bands are non-specific signal.

All lanes:

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913) at 1/2000 dilution

All lanes:

NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 175 kDa

Observed band size: 180 kDa

false

• WB

Lab

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (AB259913)

Blocking and Diluting buffer and concentration : 5% NFDM/TBST

Lanes 1-4 : Merged signal (red and green). Green - ab259913 observed at 180 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

ab259913 Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] was shown to specifically react with KDM5C / Jarid1C / SMCX in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266251 (knockout cell lysate ab257494) and ab266252 (knockout cell lysate ab257495) were used. Wild-type and KDM5C / Jarid1C / SMCX knockout samples were subjected to SDS-PAGE. ab259913 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4℃ overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913) at 1/1000 dilution

Lane 1:

Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 2:

Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-kdm5c-jarid1c-smcx-knockout-hek-293t-cell-line-ab266252'>ab266252</a>) at 20 µg

Lane 3:

Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-kdm5c-jarid1c-smcx-knockout-hek-293t-cell-line-ab266251'>ab266251</a>) at 20 µg

Lane 4:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>)

Predicted band size: 175 kDa

Observed band size: 180 kDa

false

• WB

Supplier Data

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (AB259913)

Blocking and Diluting buffer and concentration : 5% NFDM/TBST

Lanes 1-4 : Merged signal (red and green). Green - ab259913 observed at 180 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

ab259913 Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] was shown to specifically react with KDM5C / Jarid1C / SMCX in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266251 (knockout cell lysate ab257494) and ab266252 (knockout cell lysate ab257495) were used. Wild-type and KDM5C / Jarid1C / SMCX knockout samples were subjected to SDS-PAGE. ab259913 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4℃ overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913) at 1/1000 dilution

Lane 1:

Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 2:

Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-kdm5c-jarid1c-smcx-knockout-hek-293t-cell-line-ab266252'>ab266252</a>) at 20 µg

Lane 3:

Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-kdm5c-jarid1c-smcx-knockout-hek-293t-cell-line-ab266251'>ab266251</a>) at 20 µg

Lane 4:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/10000 dilution

Predicted band size: 175 kDa

Observed band size: 180 kDa

false