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AB280963

Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] - BSA and Azide free

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Rabbit Recombinant Monoclonal KDM5C / Jarid1C / SMCX antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

DXS1272E, JARID1C, SMCX, XE169, KDM5C, Lysine-specific demethylase 5C, Histone demethylase JARID1C, Jumonji/ARID domain-containing protein 1C, Protein SmcX, Protein Xe169, [histone H3]-trimethyl-L-lysine(4) demethylase 5C

3 Images
Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] - BSA and Azide free (AB280963)
  • WB

Supplier Data

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] - BSA and Azide free (AB280963)

This data was developed using ab259913, the same antibody clone in a different buffer formulation.

Blocking and Diluting buffer and concentration : Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

Exposure time 70 seconds

Smaller bands are non-specific signal.

All lanes:

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (<a href='/en-us/products/primary-antibodies/kdm5c-jarid1c-smcx-antibody-epr23932-18-ab259913'>ab259913</a>) at 1/1000 dilution

Lane 1:

HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate at 20 µg

Lane 2:

HEK293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 175 kDa

Observed band size: 180 kDa

false

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] - BSA and Azide free (AB280963)
  • WB

Supplier Data

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] - BSA and Azide free (AB280963)

This data was developed using ab259913, the same antibody clone in a different buffer formulation.

Blocking and Diluting buffer and concentration : 5% NFDM/TBST

Exposure time 3 minutes

Smaller bands are non-specific signal.

All lanes:

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (<a href='/en-us/products/primary-antibodies/kdm5c-jarid1c-smcx-antibody-epr23932-18-ab259913'>ab259913</a>) at 1/2000 dilution

All lanes:

NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 175 kDa

Observed band size: 180 kDa

false

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] - BSA and Azide free (AB280963)
  • WB

Supplier Data

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] - BSA and Azide free (AB280963)

This data was developed using ab259913, the same antibody clone in a different buffer formulation.

Blocking and Diluting buffer and concentration : 5% NFDM/TBST

Lanes 1-4 : Merged signal (red and green). Green - ab259913 observed at 180 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

Lanes 1-3 : ab259913 Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] was shown to specifically react with KDM5C / Jarid1C / SMCX in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266251 (knockout cell lysate ab257494) and ab266252 (knockout cell lysate ab257495) were used. Wild-type and KDM5C / Jarid1C / SMCX knockout samples were subjected to SDS-PAGE. ab259913 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4℃ overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (<a href='/en-us/products/primary-antibodies/kdm5c-jarid1c-smcx-antibody-epr23932-18-ab259913'>ab259913</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 2:

Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-kdm5c-jarid1c-smcx-knockout-hek-293t-cell-line-ab266252'>ab266252</a>) at 20 µg

Lane 3:

Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-kdm5c-jarid1c-smcx-knockout-hek-293t-cell-line-ab266251'>ab266251</a>) at 20 µg

Lane 4:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/10000 dilution

Predicted band size: 175 kDa

Observed band size: 180 kDa

false

  • Unconjugated

    Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23932-18

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab280963 is the carrier-free version of ab259913.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code (PubMed : 28262558). Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements. Represses the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity).
See full target information KDM5C
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com