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AB308193

Anti-KDM6A antibody [HL1731]

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Rabbit Recombinant Monoclonal KDM6A / UTX antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Cat, Dog samples. Immunogen corresponding to Recombinant Fragment Protein within Human KDM6A.

View Alternative Names

UTX, KDM6A, Lysine-specific demethylase 6A, Histone demethylase UTX, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein, [histone H3]-trimethyl-L-lysine(27) demethylase 6A

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-KDM6A antibody [HL1731] (AB308193)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KDM6A antibody [HL1731] (AB308193)

For immunofluorescence analysis, MCF-7 cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of KDM6A protein using ab308193 at 1/500 dilution (green).

Nuclei (blue) is stained with fluoroshield with DAPI

Alpha Tubulin stained by alpha Tubulin antibody (red) diluted at 1/1000

Immunohistochemistry paraffin embedded sections - Anti-KDM6A antibody [HL1731] (AB308193)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-KDM6A antibody [HL1731] (AB308193)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma labeling KDM6A protein with ab308193 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM6A antibody [HL1731] (AB308193)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM6A antibody [HL1731] (AB308193)

Immunohistochemical analysis of paraffin-embedded Cat intestine/colon tissue labelling KDM6A with ab308193 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM6A antibody [HL1731] (AB308193)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM6A antibody [HL1731] (AB308193)

Immunohistochemical analysis of paraffin-embedded dog brain tissue labelling KDM6A with ab308193 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1731

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Dog, Cat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human KDM6A. The exact immunogen used to generate this antibody is proprietary information.

O15550

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Cat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Dog": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed : 17713478, PubMed : 17761849, PubMed : 17851529). Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27' (PubMed : 17713478, PubMed : 17761849, PubMed : 17851529). Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed : 17851529). Demethylation of 'Lys-27' of histone H3 is concomitant with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A (PubMed : 17761849). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression (By similarity).
See full target information KDM6A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com