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Knockout Tested Rabbit Recombinant Monoclonal KDM6A / UTX antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.

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Images

Western blot - Anti-KDM6A / UTX antibody [EPR23203-211] - BSA and Azide free (AB275402), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-KDM6A / UTX antibody [EPR23203-211] - BSA and Azide free (AB275402), expandable thumbnail
  • Western blot - Anti-KDM6A / UTX antibody [EPR23203-211] - BSA and Azide free (AB275402), expandable thumbnail
  • Western blot - Anti-KDM6A / UTX antibody [EPR23203-211] - BSA and Azide free (AB275402), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-KDM6A / UTX antibody [EPR23203-211] - BSA and Azide free (AB275402), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPChIPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Target data

Function

Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed:17851529, PubMed:17713478, PubMed:17761849). Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27' (PubMed:17851529, PubMed:17713478, PubMed:17761849). Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed:17851529). Demethylation of 'Lys-27' of histone H3 is concomitant with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A (PubMed:17761849). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression (By similarity).

Alternative names

Lysine-specific demethylase 6A, Histone demethylase UTX, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein, [histone H3]-trimethyl-L-lysine(27) demethylase 6A, KDM6A, UTX

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal KDM6A / UTX antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR23203-211

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab275402 is the carrier-free version of Anti-KDM6A / UTX antibody [EPR23203-211] ab253183.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-KDM6A / UTX antibody [EPR23203-211] - BSA and Azide free (ab275402), expandable thumbnail

    Western blot - Anti-KDM6A / UTX antibody [EPR23203-211] - BSA and Azide free (ab275402)

    This data was developed using Anti-KDM6A / UTX antibody [EPR23203-211] ab253183, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.

    Lanes 1-4: Merged signal (red and green). Green - Anti-KDM6A / UTX antibody [EPR23203-211] ab253183 observed at 160 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

    Anti-KDM6A / UTX antibody [EPR23203-211] ab253183 Anti-KDM6A antibody [EPR23203-211] was shown to react with KDM6A in HAP1 wild-type cells in Western blot. Loss of signal was observed when KDM6A knockout sample was used. Wild-type and KDM6A knockout samples were subjected to SDS-PAGE.

    Anti-KDM6A / UTX antibody [EPR23203-211] ab253183 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2. 5 hours at 1/1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    Negative control: THP-1 (PMID: 19330029).

    The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 19330029).

    The the bands between 50kDa and 150kDa are caused by degradation based on the result of fresh lysates.

    All lanes: Western blot - Anti-KDM6A / UTX antibody [EPR23203-211] (Anti-KDM6A / UTX antibody [EPR23203-211] ab253183) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: KDM6A knockout HAP1 whole cell lysate at 20 µg

    Lane 3: F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution

    Predicted band size: 154 kDa

    Observed band size: 160 kDa

  • Flow Cytometry (Intracellular) - Anti-KDM6A / UTX antibody [EPR23203-211] - BSA and Azide free (ab275402), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-KDM6A / UTX antibody [EPR23203-211] - BSA and Azide free (ab275402)

    This data was developed using Anti-KDM6A / UTX antibody [EPR23203-211] ab253183, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte), (Left panel) / HAP1 (Human chronic myelogenous leukemia near-haploid cell line), (Right panel) cells labelling KDM6A / UTX with Anti-KDM6A / UTX antibody [EPR23203-211] ab253183 at 1/50 dilution (1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control:THP-1.

  • Western blot - Anti-KDM6A / UTX antibody [EPR23203-211] - BSA and Azide free (ab275402), expandable thumbnail

    Western blot - Anti-KDM6A / UTX antibody [EPR23203-211] - BSA and Azide free (ab275402)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Fresh lysates were used in this WB.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-KDM6A / UTX antibody [EPR23203-211] (Anti-KDM6A / UTX antibody [EPR23203-211] ab253183) at 1/1000 dilution

    Lane 1: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 154 kDa

    Observed band size: 160 kDa

  • Western blot - Anti-KDM6A / UTX antibody [EPR23203-211] - BSA and Azide free (ab275402), expandable thumbnail

    Western blot - Anti-KDM6A / UTX antibody [EPR23203-211] - BSA and Azide free (ab275402)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-KDM6A / UTX antibody [EPR23203-211] ab253183).

    Western blot: Anti-KDM6A antibody [EPR23203-211] (Anti-KDM6A / UTX antibody [EPR23203-211] ab253183) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-KDM6A / UTX antibody [EPR23203-211] ab253183 was shown to bind specifically to KDM6A. A band was observed at 150-160 kDa in wild-type A549 cell lysates with no signal observed at this size in KDM6A knockout cell line. To generate this image, wild-type and KDM6A knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-KDM6A / UTX antibody [EPR23203-211] (Anti-KDM6A / UTX antibody [EPR23203-211] ab253183) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 7 µg

    Lane 2: KDM6A knockout A549 cell lysate at 7 µg

    Lane 3: Wild-type HAP1 cell lysate at 7 µg

    Lane 4: KDM6A knockout HAP1 cell lysate at 7 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 154 kDa

    Observed band size: 150 kDa, 160 kDa

  • Flow Cytometry (Intracellular) - Anti-KDM6A / UTX antibody [EPR23203-211] - BSA and Azide free (ab275402), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-KDM6A / UTX antibody [EPR23203-211] - BSA and Azide free (ab275402)

    This data was developed using Anti-KDM6A / UTX antibody [EPR23203-211] ab253183, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized F9 (Mouse embryonal carcinoma epithelial cell) cells labelling KDM6A / UTX with Anti-KDM6A / UTX antibody [EPR23203-211] ab253183 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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