Anti-KDS antibody [EPR4947(2)] - BSA and Azide free

• WB

Lab

Western blot - Anti-KDS antibody [EPR4947(2)] - BSA and Azide free (AB248958)

This data was developed using the same antibody clone in a different buffer formulation (ab150388).

Lanes 1- 4 : Merged signal (red and green). Green - ab150388 observed at 105 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab150388 was shown to react with KDS in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265108 (knockout cell lysate ab258222) was used. Wild-type HeLa and TAOK3 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab150388 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-KDS antibody [EPR4947(2)] (<a href='/en-us/products/primary-antibodies/kds-antibody-epr49472-ab150388'>ab150388</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 40 µg

Lane 2:

TAOK3 knockout HeLa cell lysate at 40 µg

Lane 2:

Western blot - Human TAOK3 (KDS) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-taok3-kds-knockout-hela-cell-line-ab265108'>ab265108</a>)

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

Daudi cell lysate at 10 µg

Predicted band size: 105 kDa

Observed band size: 105 kDa

false

• WB

Unknown

Western blot - Anti-KDS antibody [EPR4947(2)] - BSA and Azide free (AB248958)

This data was developed using ab150388, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-KDS antibody [EPR4947(2)] (<a href='/en-us/products/primary-antibodies/kds-antibody-epr49472-ab150388'>ab150388</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit antibody at 1/2000 dilution

Predicted band size: 105 kDa

false