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AB313545

Anti-KDS antibody [RP23040255]

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Rabbit Recombinant Multiclonal KDS antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TAOK3.

View Alternative Names

DPK, JIK, KDS, MAP3K18, TAOK3, Serine/threonine-protein kinase TAO3, Cutaneous T-cell lymphoma-associated antigen HD-CL-09, Dendritic cell-derived protein kinase, JNK/SAPK-inhibitory kinase, Jun kinase-inhibitory kinase, Kinase from chicken homolog A, Thousand and one amino acid protein 3, CTCL-associated antigen HD-CL-09, hKFC-A

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-KDS antibody [RP23040255] (AB313545)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KDS antibody [RP23040255] (AB313545)

Immunofluorescent analysis of LNCaP cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling JIK with ab313545 at 1/100 dilution and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate (Product # A27034, 1/2000). Nuclei (blue) were stained using DAPI , and Rhodamine Phalloidin at 1/300 dilution was used for cytoskeletal F-actin (red) staining. Panel a-d) clearly demonstrates membrane localization of JIK in LNCaP cells. Panel e-h) shows no specific staining in DU145 cells which is JIK negative, demonstrating specificity. The images were captured at 60X magnification.

Western blot - Anti-KDS antibody [RP23040255] (AB313545)
  • WB

Supplier Data

Western blot - Anti-KDS antibody [RP23040255] (AB313545)

All lanes:

Western blot - Anti-KDS antibody [RP23040255] (ab313545) at 1/330 dilution

Lane 1:

Raji cell lysate at 30 µg

Lane 2:

Jurkat cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

Observed band size: 115 kDa

false

Key facts

Host species

Rabbit

Clonality

Multiclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TAOK3.

Q9H2K8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/330", "WB-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: 99.91% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase that acts as a regulator of the p38/MAPK14 stress-activated MAPK cascade and of the MAPK8/JNK cascade. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Inhibits basal activity of MAPK8/JNK cascade and diminishes its activation in response epidermal growth factor (EGF).
See full target information TAOK3
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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