AB139729

Anti-Keap1 antibody

Name: Anti-Keap1 antibody (ab139729) | Abcam
Brand: Abcam Limited
SKU: AB139729
Price: 645 USD
Availability: InStock

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(67 Publications)

Rabbit Polyclonal KEAP1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 67 publications. Immunogen corresponding to Synthetic Peptide within Human KEAP1 aa 550 to C-terminus.

View Alternative Names

INRF2, KIAA0132, KLHL19, KEAP1, Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, Kelch-like protein 19, INrf2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Keap1 antibody (AB139729)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Keap1 antibody (AB139729)

Immunofluorescence of Keap1 in human lung tissue with ab139727 at 20μg/mL.

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Western blot - Anti-Keap1 antibody (AB139729)

Lane 1:

Western blot - Anti-Keap1 antibody (ab139729) at 1 µg/mL

Lane 2:

Western blot - Anti-Keap1 antibody (ab139729) at 2 µg/mL

All lanes:

Human lung tissue lysate at 15 µg

Predicted band size: 70 kDa

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Supplier Data

Western blot - Anti-Keap1 antibody (AB139729)

Incubated with the primary antibody for 1 hour at room temperature in 5% NFDM/TBST.

Incubated with anti-beta actin at 1μg/ml.

All lanes:

Western blot - Anti-Keap1 antibody (ab139729) at 2 µg/mL

Lane 1:

Wild-type HeLa cell lysate at 10 µg

Lane 2:

KEAP1 knockout HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution

Predicted band size: 70 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human KEAP1 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14145

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Keap1 also known as Kelch-like ECH-associated protein 1 plays an important role in regulating the NRF2 protein. Keap1 has a molecular weight of around 70 kDa. It expresses widely throughout mammalian tissues reflecting its significance in cellular functions. As a substrate adaptor protein for the cullin-3-based E3 ubiquitin ligase complex its main function involves the promotion of NRF2 degradation. Keap1 recognizes and binds to specific motifs on NRF2 tagging it for ubiquitination and subsequent proteasomal degradation under homeostatic conditions.

Biological function summary

Keap1 serves as a sensor for oxidative stress within the cell. It constitutes part of a larger biological complex that includes the NRF2 protein and the cullin-3-based E3 ligase complex. In the presence of oxidative or electrophilic stress modifications in Keap1 cysteine thiol groups lead to conformational changes inhibiting Keap1’s ability to target NRF2 for degradation. This results in the accumulation and activation of NRF2 which migrates to the nucleus where it promotes the expression of antioxidant response element (ARE)-driven genes important for cellular defense mechanisms.

Pathways

Keap1 plays a central role in the NRF2-ARE signaling pathway a major player in the antioxidant response of cells. This pathway connects to various processes including detoxification and the regulation of oxidative stress. Keap1 interacts with other proteins such as cullin-3 which is vital for ubiquitin tagging of NRF2. Additionally Keap1 influences the WNT signaling pathway through its interactions with other proteins impacting processes related to cellular proliferation and differentiation.

Keap1 relates prominently to certain cancers and neurodegenerative diseases. Mutations or dysregulations in Keap1 or its pathway can lead to abnormal accumulation of NRF2 potentially resulting in enhanced cell survival and proliferation which is characteristic of some cancer types. Moreover Keap1 has been linked to neurodegenerative disorders like Alzheimer's disease where oxidative stress and disrupted NRF2 signaling play contributory roles. Connections within these conditions often involve proteins like NRF2 which interacts closely with Keap1 in the context of pathophysiological cellular responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination (PubMed : 14585973, PubMed : 15379550, PubMed : 15572695, PubMed : 15601839, PubMed : 15983046, PubMed : 37339955). KEAP1 acts as a key sensor of oxidative and electrophilic stress : in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes (PubMed : 15601839, PubMed : 16006525). In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes (PubMed : 16006525, PubMed : 17127771, PubMed : 18251510, PubMed : 19489739, PubMed : 29590092). In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2 (PubMed : 20452972). The BCR(KEAP1) complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation (PubMed : 28380357). The BCR(KEAP1) complex also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome (PubMed : 15379550, PubMed : 17046835).

See full target information KEAP1

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Publications (67)

Recent publications for all applications. Explore the full list and refine your search

Redox report : communications in free radical research 30:2529618 PubMed40669859

2025

ACSF2-PGK1 interaction promotes ferroptosis in renal tubular epithelial cells of diabetic nephropathy by regulating Keap1/Nrf2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xinran Liu,Chaoyi Chen,Sai Zhu,Xiaomei Luo,Li Gao,Ziyun Hu,Yu Ma,Ling Jiang,Yonggui Wu

International journal of molecular sciences 26: PubMed39859216

2025

Gboxin Induced Apoptosis and Ferroptosis of Cervical Cancer Cells by Promoting Autophagy-Mediated Inhibition of Nrf2 Signaling Under Low-Glucose Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Junlin Lu,Jiarui Li,Lu Wang,Yao Chen,Yulun Wu,Ziying Zhang,Jingying Zhang,Feng Gao,Chaoran Jia,Yongli Bao,Xiaoguang Yang,Zhenbo Song

Journal of biochemical and molecular toxicology 38:e23808 PubMed39132830

2024

Calycosin activates Nrf2/Keap1 signaling to ameliorate hydrogen peroxide-induced spinal cord neuron death and mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Ruan,Ziming Cai,Zhengwen Kang,Jinzhu Liang,He Tian,Qinghe Yu,Qiusheng Zhang,Wenping Lin

Experimental and therapeutic medicine 28:330 PubMed38979021

2024

Prevention of supercritical carbon dioxide fluid extract from Linnén on cutaneous squamous cell carcinomas progression following UV irradiation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Hong Luo,Hong-Juan Chen,Qing-Yuan Zhong,Hao-En He,Ying-Qi Huang,You-Chen Liu,Bin Lan,Yao-Qi Wen,Si-Liang Deng,Xian-Hua Du,Bao-Qin Lin,Ya-Xian Zhan

Experimental hematology & oncology 13:30 PubMed38468359

2024

Suppression of A-to-I RNA-editing enzyme ADAR1 sensitizes hepatocellular carcinoma cells to oxidative stress through regulating Keap1/Nrf2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Houhong Wang,Xiaoyu Wei,Lu Liu,Junfeng Zhang,Heng Li

Pharmaceutics 15: PubMed38140028

2023

Modular Nanotransporters Delivering Biologically Active Molecules to the Surface of Mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Yuri V Khramtsov,Alexey V Ulasov,Tatiana A Slastnikova,Andrey A Rosenkranz,Tatiana N Lupanova,Georgii P Georgiev,Alexander S Sobolev

Communications biology 6:676 PubMed37380734

2023

Spermidine from arginine metabolism activates Nrf2 and inhibits kidney fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Seishi Aihara,Kumiko Torisu,Yushi Uchida,Noriyuki Imazu,Toshiaki Nakano,Takanari Kitazono

Environmental toxicology 38:1678-1689 PubMed37087754

2023

NEDD4L inhibits migration, invasion, cisplatin resistance and promotes apoptosis of bladder cancer cells by inactivating the p62/Keap1/Nrf2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wu,Huijiang Zhang,Shengjie You,Zhaoyu Xu,Xiang Liu,Xuedong Chen,Weili Zhang,Junjie Ye,Peng Li,Xiaoqing Zhou

Pharmaceutics 15: PubMed36839653

2023

An Approach to Evaluate the Effective Cytoplasmic Concentration of Bioactive Agents Interacting with a Selected Intracellular Target Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Yuri V Khramtsov,Alexey V Ulasov,Andrey A Rosenkranz,Tatiana A Slastnikova,Tatiana N Lupanova,Georgii P Georgiev,Alexander S Sobolev

American journal of translational research 15:392-406 PubMed36777848

2023

miR-432 exerts a protective effect against myocardial ischemia/reperfusion injury by activating the β-catenin/HIF-1α pathway and augmenting NRF2-mediated anti-oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Rui-Ying Su,Wei-Yan Tai,Hong-Shan Yin,Xiao-Yong Geng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Keap1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Keap1 antibody (AB139729)

Western blot - Anti-Keap1 antibody (AB139729)

Western blot - Anti-Keap1 antibody (AB139729)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (67)

Product promise