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AB226997

Anti-Keap1 antibody

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(8 Publications)

Rabbit Polyclonal KEAP1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KEAP1.

View Alternative Names

INRF2, KIAA0132, KLHL19, KEAP1, Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, Kelch-like protein 19, INrf2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Keap1 antibody (AB226997)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Keap1 antibody (AB226997)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Keap1 (green) using ab226997 at 1/500 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Western blot - Anti-Keap1 antibody (AB226997)
  • WB

Supplier Data

Western blot - Anti-Keap1 antibody (AB226997)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Keap1 antibody (ab226997) at 1/1000 dilution

All lanes:

NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human KEAP1. The exact immunogen used to generate this antibody is proprietary information.

Q14145

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Keap1 also known as Kelch-like ECH-associated protein 1 plays an important role in regulating the NRF2 protein. Keap1 has a molecular weight of around 70 kDa. It expresses widely throughout mammalian tissues reflecting its significance in cellular functions. As a substrate adaptor protein for the cullin-3-based E3 ubiquitin ligase complex its main function involves the promotion of NRF2 degradation. Keap1 recognizes and binds to specific motifs on NRF2 tagging it for ubiquitination and subsequent proteasomal degradation under homeostatic conditions.
Biological function summary

Keap1 serves as a sensor for oxidative stress within the cell. It constitutes part of a larger biological complex that includes the NRF2 protein and the cullin-3-based E3 ligase complex. In the presence of oxidative or electrophilic stress modifications in Keap1 cysteine thiol groups lead to conformational changes inhibiting Keap1’s ability to target NRF2 for degradation. This results in the accumulation and activation of NRF2 which migrates to the nucleus where it promotes the expression of antioxidant response element (ARE)-driven genes important for cellular defense mechanisms.

Pathways

Keap1 plays a central role in the NRF2-ARE signaling pathway a major player in the antioxidant response of cells. This pathway connects to various processes including detoxification and the regulation of oxidative stress. Keap1 interacts with other proteins such as cullin-3 which is vital for ubiquitin tagging of NRF2. Additionally Keap1 influences the WNT signaling pathway through its interactions with other proteins impacting processes related to cellular proliferation and differentiation.

Keap1 relates prominently to certain cancers and neurodegenerative diseases. Mutations or dysregulations in Keap1 or its pathway can lead to abnormal accumulation of NRF2 potentially resulting in enhanced cell survival and proliferation which is characteristic of some cancer types. Moreover Keap1 has been linked to neurodegenerative disorders like Alzheimer's disease where oxidative stress and disrupted NRF2 signaling play contributory roles. Connections within these conditions often involve proteins like NRF2 which interacts closely with Keap1 in the context of pathophysiological cellular responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination (PubMed : 14585973, PubMed : 15379550, PubMed : 15572695, PubMed : 15601839, PubMed : 15983046, PubMed : 37339955). KEAP1 acts as a key sensor of oxidative and electrophilic stress : in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes (PubMed : 15601839, PubMed : 16006525). In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes (PubMed : 16006525, PubMed : 17127771, PubMed : 18251510, PubMed : 19489739, PubMed : 29590092). In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2 (PubMed : 20452972). The BCR(KEAP1) complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation (PubMed : 28380357). The BCR(KEAP1) complex also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome (PubMed : 15379550, PubMed : 17046835).
See full target information KEAP1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 29:155 PubMed39911153

2025

Construction of a lung adenocarcinoma prognostic model based on KEAP1/NRF2/HO‑1 mutation‑mediated upregulated genes and bioinformatic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhu,Ye Zhang,Lingyun Yang,Lu Chen,Chaobo Chen,Qifeng Shi,Zipeng Xu

PloS one 19:e0311819 PubMed39388445

2024

The antioxidant betulinic acid enhances porcine oocyte maturation through Nrf2/Keap1 signaling pathway modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Min Ju Kim,Hyo-Gu Kang,Se-Been Jeon,Ji Hyeon Yun,Pil-Soo Jeong,Bo-Woong Sim,Sun-Uk Kim,Seong-Keun Cho,Bong-Seok Song

Journal of animal science and biotechnology 14:148 PubMed38037099

2023

Enhancement of porcine in vitro embryonic development through luteolin-mediated activation of the Nrf2/Keap1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Se-Been Jeon,Pil-Soo Jeong,Min Ju Kim,Hyo-Gu Kang,Bong-Seok Song,Sun-Uk Kim,Seong-Keun Cho,Bo-Woong Sim

Frontiers in endocrinology 13:962303 PubMed36120426

2022

Hydrogen saline water accelerates fracture healing by suppressing autophagy in ovariectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jialiang Guo,Siyu Tian,Zhongzheng Wang,Yuchuan Wang,Xiaojuan Zhang,Yingze Zhang,Zhiyong Hou,Weichong Dong

Journal of assisted reproduction and genetics 39:995-1008 PubMed35237893

2022

Randomized controlled trial of astaxanthin impacts on antioxidant status and assisted reproductive technology outcomes in women with polycystic ovarian syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Roghaye Gharaei,Ashraf Alyasin,Forough Mahdavinezhad,Esmaeil Samadian,Zhaleh Ashrafnezhad,Fardin Amidi

Bioengineered 13:3526-3536 PubMed35200072

2022

Knockdown of nuclear receptor binding SET domain-containing protein 1 (NSD1) inhibits proliferation and facilitates apoptosis in paclitaxel-resistant breast cancer cells via inactivating the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Chen,Xiao Li,Jin Xu,Hua Xiao,Cuiju Tang,Wei Liang,Xuedan Zhu,Yueyu Fang,Hanjin Wang,Junfeng Shi

Experimental and therapeutic medicine 23:226 PubMed35222703

2022

Inhibition of thioredoxin-interacting protein may enhance the therapeutic effect of dehydrocostus lactone in cardiomyocytes under doxorubicin stimulation via the inhibition of the inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Xuezhi Zhang,Cuiyu Chu,Yuankun Huang

Science advances 5:eaav4570 PubMed31032410

2019

The protein kinase activity of fructokinase A specifies the antioxidant responses of tumor cells by phosphorylating p62.

Applications

Unspecified application

Species

Unspecified reactive species

Daqian Xu,Xinjian Li,Fei Shao,Guishuai Lv,Hongwei Lv,Jong-Ho Lee,Xu Qian,Zheng Wang,Yan Xia,Linyong Du,Yanhua Zheng,Hongyang Wang,Jianxin Lyu,Zhimin Lu
View all publications
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Product promise

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