AB124975

Anti-Keratin 12/K12 antibody [EPR1609(2)]

RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal K1C12 antibody. Suitable for WB and reacts with Rat samples. Cited in 7 publications.

View Alternative Names

Cytokeratin-12, Keratin-12, CK-12, K12, KRT12

1 Images

Unknown

Western blot - Anti-Keratin 12/K12 antibody [EPR1609(2)] (AB124975)

All lanes:

Western blot - Anti-Keratin 12/K12 antibody [EPR1609(2)] (ab124975) at 1/1000 dilution

All lanes:

Rat eyeball lysate at 10 µg

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1609(2)

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Keratin 12 (K12) also called keratin or keratin 1.12 is a type I cytokeratin protein with a mass of approximately 52 kDa. K12 is specifically expressed in the corneal epithelium an important part of the eye's outer structure. This expression is restricted making K12 a marker for corneal epithelial cells. Anti-keratin antibodies can help identify and visualize this protein in cytology studies.

Biological function summary

Keratin 12 is essential in maintaining the structural integrity and mechanical resilience of corneal epithelial cells. It forms part of the intermediate filament complex with other keratins providing a framework essential in cell stability and protection. The complex strengthens cell adhesion and maintains the corneal epithelium's hydration status enabling clear vision.

Pathways

The formation and maintenance of the corneal epithelium involve Keratin 12 in particular cellular signaling pathways. The protein takes part in the intermediate filament pathway interacting with proteins like Keratin 3. These interactions support the proper assembly and function of the cytoskeletal structure essential for cell shape and resilience significantly impacting corneal wound healing processes.

Keratin 12 mutations can lead to Meesmann corneal dystrophy which affects vision. This condition involves aberrant keratin filament assembly compromising corneal integrity. Keratin 3 often partners with K12 in maintaining corneal coherence and mutations affecting either protein can contribute to the disorder highlighting their interconnected roles in corneal health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in corneal epithelium organization, integrity and corneal keratin expression.

See full target information KRT12

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Current issues in molecular biology 45:10121-10130 PubMed38132478

2023

NRG1 Regulates Proliferation, Migration and Differentiation of Human Limbal Epithelial Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bofeng Wang,Huizhen Guo,Zhuo Han,Siqi Wu,Jiafeng Liu,Zesong Lin,Fengjiao An,Jin Zhu,Mingsen Li

Nature communications 12:420 PubMed33462242

2021

Core transcription regulatory circuitry orchestrates corneal epithelial homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Mingsen Li,Huaxing Huang,Lingyu Li,Chenxi He,Liqiong Zhu,Huizhen Guo,Li Wang,Jiafeng Liu,Siqi Wu,Jingxin Liu,Tao Xu,Zhen Mao,Nan Cao,Kang Zhang,Fei Lan,Junjun Ding,Jin Yuan,Yizhi Liu,Hong Ouyang

Signal transduction and targeted therapy 6:5 PubMed33414365

2021

Loss of FOXC1 contributes to the corneal epithelial fate switch and pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mingsen Li,Liqiong Zhu,Jiafeng Liu,Huaxing Huang,Huizhen Guo,Li Wang,Lingyu Li,Sijie Gu,Jieying Tan,Jing Zhong,Bowen Wang,Zhen Mao,Yong Fan,Chunqiao Liu,Jin Yuan,Hong Ouyang

Investigative ophthalmology & visual science 59:952-966 PubMed29450535

2018

A Spatiotemporal Requirement for Prickle 1-Mediated PCP Signaling in Eyelid Morphogenesis and Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Dianlei Guo,Zhaohui Yuan,Jiali Ru,Xinyu Gu,Wang Zhang,Fuxiang Mao,Hong Ouyang,Kaili Wu,Yizhi Liu,Chunqiao Liu

Experimental eye research 154:30-38 PubMed27818314

2016

PAX6 regulates human corneal epithelium cell identity.

Applications

Unspecified application

Species

Unspecified reactive species

Koji Kitazawa,Takafusa Hikichi,Takahiro Nakamura,Chie Sotozono,Shigeru Kinoshita,Shinji Masui

The Journal of biological chemistry 290:20448-54 PubMed26045558

2015

Transcription Factor PAX6 (Paired Box 6) Controls Limbal Stem Cell Lineage in Development and Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Gen Li,Fan Xu,Jie Zhu,Michal Krawczyk,Ying Zhang,Jin Yuan,Sherrinal Patel,Yujuan Wang,Ying Lin,Ming Zhang,Huimin Cai,Daniel Chen,Meixia Zhang,Guiqun Cao,Emily Yeh,Danni Lin,Qiao Su,Wen-wen Li,George L Sen,Natalie Afshari,Shaochen Chen,Richard L Maas,Xiang-Dong Fu,Kang Zhang,Yizhi Liu,Hong Ouyang

Nature 511:358-61 PubMed25030175

2014

WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Ouyang,Yuanchao Xue,Ying Lin,Xiaohui Zhang,Lei Xi,Sherrina Patel,Huimin Cai,Jing Luo,Meixia Zhang,Ming Zhang,Yang Yang,Gen Li,Hairi Li,Wei Jiang,Emily Yeh,Jonathan Lin,Michelle Pei,Jin Zhu,Guiqun Cao,Liangfang Zhang,Benjamin Yu,Shaochen Chen,Xiang-Dong Fu,Yizhi Liu,Kang Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Keratin 12/K12 antibody [EPR1609(2)]

Western blot - Anti-Keratin 12/K12 antibody [EPR1609(2)] (AB124975)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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