AB250843

Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal K1C12 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Rat, Transfected cell line - Human, Mouse, Human samples. Cited in 1 publication.

View Alternative Names

Cytokeratin-12, Keratin-12, CK-12, K12, KRT12

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

This data was developed using ab185626, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Keratin 12/K12 with ab185626 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on Human kidney.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

This data was developed using ab185626, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cornea tissue labeling Keratin 12/K12 with ab185626 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on epithelium of Human cornea is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

This data was developed using ab185626, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded sagittal section of mouse cornea tissue labeling Keratin 12/K12 with ab185626 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on epithelium of mouse cornea is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

This data was developed using ab185626, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded sagittal section of Rat cornea tissue labeling Keratin 12/K12 with ab185626 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on epithelium of rat cornea is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

This data was developed using ab185626, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Keratin 12/K12 with ab185626 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on mouse cerebrum.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

This data was developed using ab185626, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Keratin 12/K12 with ab185626 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on rat liver.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

This data was developed using ab185626, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Keratin 12/K12 is cornea specific (PMID : 8759347).

All lanes:

Western blot - Anti-Keratin 12/K12 antibody [EPR17883] (<a href='/en-us/products/primary-antibodies/keratin-12-k12-antibody-epr17883-ab185626'>ab185626</a>) at 1/2000 dilution

Lane 1:

Rat eyeball lysate at 20 µg

Lane 2:

Mouse eyeball lysate at 20 µg

Lane 3:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 5:

Mouse heart lysate at 20 µg

Lane 6:

Rat kidney lysate at 20 µg

Lane 7:

Mouse spleen lysate at 20 µg

Lane 8:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 9:

Y79 (Human retinoblastoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 54 kDa

Observed band size: 54 kDa

false

Exposure time: 54s

Unknown

Western blot - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

This data was developed using ab185626, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Keratin 12/K12 antibody [EPR17883] (<a href='/en-us/products/primary-antibodies/keratin-12-k12-antibody-epr17883-ab185626'>ab185626</a>) at 1/20000 dilution

Lane 1:

Empty vector (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

Lane 2:

Human Keratin 12/K12 transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 41 kDa,54 kDa

Observed band size: 54 kDa,55-60 kDa

false

Exposure time: 2s

Unconjugated

Anti-Keratin 12/K12 antibody [EPR17883]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17883

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab250843 is the carrier-free version of ab185626.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Keratin 12 (K12) also called keratin or keratin 1.12 is a type I cytokeratin protein with a mass of approximately 52 kDa. K12 is specifically expressed in the corneal epithelium an important part of the eye's outer structure. This expression is restricted making K12 a marker for corneal epithelial cells. Anti-keratin antibodies can help identify and visualize this protein in cytology studies.

Biological function summary

Keratin 12 is essential in maintaining the structural integrity and mechanical resilience of corneal epithelial cells. It forms part of the intermediate filament complex with other keratins providing a framework essential in cell stability and protection. The complex strengthens cell adhesion and maintains the corneal epithelium's hydration status enabling clear vision.

Pathways

The formation and maintenance of the corneal epithelium involve Keratin 12 in particular cellular signaling pathways. The protein takes part in the intermediate filament pathway interacting with proteins like Keratin 3. These interactions support the proper assembly and function of the cytoskeletal structure essential for cell shape and resilience significantly impacting corneal wound healing processes.

Keratin 12 mutations can lead to Meesmann corneal dystrophy which affects vision. This condition involves aberrant keratin filament assembly compromising corneal integrity. Keratin 3 often partners with K12 in maintaining corneal coherence and mutations affecting either protein can contribute to the disorder highlighting their interconnected roles in corneal health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in corneal epithelium organization, integrity and corneal keratin expression.

See full target information KRT12

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

Western blot - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

Western blot - Anti-Keratin 12/K12 antibody [EPR17883] - BSA and Azide free (AB250843)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise