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AB111599

Anti-Keratin 36 antibody

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(2 Publications)

Rabbit Polyclonal Keratin 36 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KRT36 aa 1-200.

View Alternative Names

HHA6, HKA6, KRTHA6, KRT36, Keratin-36, K36

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Keratin 36 antibody (AB111599)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Keratin 36 antibody (AB111599)

ab111599 at 1/200 dilution staining Keratin 36 in methanol-fixed HeLa cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.

Western blot - Anti-Keratin 36 antibody (AB111599)
  • WB

Unknown

Western blot - Anti-Keratin 36 antibody (AB111599)

10% SDS-PAGE.

All lanes:

Western blot - Anti-Keratin 36 antibody (ab111599) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KRT36 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

O76013

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab111599 is purified by antigen affinity chromatography.
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Keratin 36 also known as KRT36 or K6HF is a type I keratin protein within the keratin family. The protein has a mass of approximately 50 kDa. This protein primarily expresses in hair follicles particularly in the inner root sheath and the hair shaft cortex. Keratin 36 contributes to the mechanical stability and integrity of hair fibers assisting in forming and maintaining the strength and resilience of the hair structure.
Biological function summary

Keratin 36 serves an important role in the structural framework of epithelial cells specifically those contributing to keratinocyte function and hair formation. It forms intermediate filaments as part of a larger keratin complex interacting with other keratins such as type II hard keratins in hair and nails. These interactions help establish the strong and insoluble nature of the protective barriers where keratin is present.

Pathways

Keratin 36 participates in intricate signaling related to hair follicle morphogenesis and differentiation. It is involved in the epithelial structural complex working alongside pathways such as the Wnt signaling pathway and the epidermal growth factor (EGF) signaling pathway. Related proteins like Keratin 35 and Keratin 85 also play essential roles in these pathways further integrating with the processes governing hair and skin tissue formation.

Keratin 36 mutations or dysregulation link to conditions like hair disorders and keratoderma. Specifically hereditary conditions such as monilethrix characterized by brittle beaded hair show associations with alterations in keratin expression patterns. Keratin 36 interacts with proteins like Filaggrin and Loricrin in these contexts which are also involved in related structural disorders of the skin.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information KRT36
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 41:e110145 PubMed35349166

2022

K27-linked ubiquitylation promotes p97 substrate processing and is essential for cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Robert F Shearer,Dimitris Typas,Fabian Coscia,Sofie Schovsbo,Thomas Kruse,Andreas Mund,Niels Mailand

Microbial biotechnology 13:1103-1117 PubMed32227590

2020

Exosome-derived uterine miR-218 isolated from cows with endometritis regulates the release of cytokines and chemokines.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangguo Wang,Xinxin Yao,Tongtong Xie,Zhenyu Chang,Yong Guo,Hemin Ni
View all publications
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Product promise

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