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AB273139

Anti-Keratin 77 antibody [KRTL/4440R]

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Rabbit Recombinant Monoclonal K2C1B antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human KRT77 aa 100-200.

View Alternative Names

KRT1B, KRT77, Cytokeratin-1B, Keratin-77, Type-II keratin Kb39, CK-1B, K77

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 77 antibody [KRTL/4440R] (AB273139)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 77 antibody [KRTL/4440R] (AB273139)

Paraffin embedded human colon carcinoma tissue stained for Cytokeratin type I using ab273139 at 2 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 77 antibody [KRTL/4440R] (AB273139)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratin 77 antibody [KRTL/4440R] (AB273139)

Paraffin embedded human colon carcinoma tissue stained for Cytokeratin type I using ab273139 at 2 μg/ml in immunohistochemical analysis.

  • Carrier free

    Anti-Keratin 77 antibody [KRTL/4440R] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

KRTL/4440R

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human KRT77 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q7Z794

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Keratin 77 also known as KRT77 or CYK17 is a type of keratin protein that plays a structural role in epithelial cells. This protein weighs approximately 56 kDa and is mainly expressed in the suprabasal layers of the epidermis and in hair follicles where it contributes to the formation and maintenance of cellular integrity. Keratin 77 belongs to the type II keratin family which pairs with type I keratins to form intermediate filaments essential for cell stability and resilience against mechanical stress.
Biological function summary

Keratin 77 functions as a critical component in skin barrier function and keratinocyte differentiation. It integrates into complex networks of intermediate filaments that provide tensile strength to cells. This keratin participates in maintaining the rigidity and elasticity of the skin by combining with other keratins like KRT5 and KRT14 constituting a keratin filament complex necessary for proper skin function. The presence of keratin 77 in hair follicles also suggests a role in hair structure and growth.

Pathways

Keratin 77 is deeply involved in several skin differentiation and repair pathways. It interacts with proteins such as filaggrin and loricrin in the epidermal differentiation complex supporting the skin barrier's protective function. In the wound healing pathway keratin 77 modulates cellular response and organization within the epidermis working alongside proteins like involucrin to facilitate skin repair processes.

Mutations or deficiencies in keratin 77 have associations with skin-related conditions including dermatitis and pachyonychia congenita. In dermatitis abnormal keratin 77 expression affects skin barrier function potentially exacerbating inflammatory responses. Additionally the role of keratin 77 in pachyonychia congenita links it to nail dystrophies where it interacts with keratins such as KRT16 and KRT6A contributing to the clinical manifestations of this disorder.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information KRT77
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