Rabbit Polyclonal Keratinocyte differentiation associated protein antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KRTDAP aa 1 to C-terminus.
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- Oncology letters 21:2892021PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV-negative head and neck squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYushen Su,Zhirui Zeng,Dongyun Rong,Yushi Yang,Bei Wu,Yu CaoPubMed 33732365
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KRTDAP aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P60985
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target data
Function
May act as a soluble regulator of keratinocyte differentiation. May play an important role in embryonic skin morphogenesis.
Alternative names
KDAP, UNQ467/PRO826, KRTDAP, Keratinocyte differentiation-associated protein
Recommended products
Rabbit Polyclonal Keratinocyte differentiation associated protein antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KRTDAP aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KRTDAP aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P60985
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Keratinocyte differentiation associated protein also known as KDAP is a protein integral to the process of skin cell differentiation. This protein has a mass of around 25 kDa and is expressed predominantly in the epidermis where keratinocytes reside. KDAP plays a role in the regulation of keratinocyte function and the formation of the outermost layer of skin the stratum corneum. The presence of KDAP has been observed across various layers of the epidermis suggesting its involvement in the progressive stages of keratinocyte maturation.
- Biological function summary
This protein enhances the differentiation process of keratinocytes important for maintaining skin barrier function and homeostasis. KDAP does not operate as part of a larger complex but independently regulates specific signaling pathways pivotal for keratinocyte histology. It participates in cellular activities that drive keratinocyte image formation ensuring orderly stratification and keratin composition. These functions are critical for developing functional and resilient skin.
- Pathways
KDAP is a significant player in pathways associated with skin development and repair. One essential pathway includes the Notch signaling pathway which involves linked proteins like Notch receptors that influence cell fate determination and differentiation. Another pathway is the epidermal growth factor receptor (EGFR) pathway where KDAP's activity connects to related proteins like EGFR that regulate cell proliferation and survival. These pathways highlight KDAP's role in maintaining the balance between proliferation and differentiation integral to healthy skin formation and response to damage.
- Associated diseases and disorders
KDAP shows associations with skin-related conditions such as psoriasis and atopic dermatitis. Psoriasis involves abnormal keratinocyte proliferation which could be linked to KDAP's regulatory functions. The protein's dysregulation could also affect atopic dermatitis a disorder associated with skin barrier dysfunction. In these cases proteins such as filaggrin a vital component of the skin barrier interact within the same framework suggesting that KDAP may influence or reflect changes in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratinocyte differentiation associated protein antibody (ab204583)
Paraffin embedded human cervix tissue stained for Keratinocyte differentiation associated protein using ab204583 at 1/2500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratinocyte differentiation associated protein antibody (ab204583)
Paraffin embedded human skin tissue stained for Keratinocyte differentiation associated protein using ab204583 at 1/2500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratinocyte differentiation associated protein antibody (ab204583)
Paraffin embedded human pancreas tissue stained for Keratinocyte differentiation associated protein using ab204583 at 1/2500 dilution in immunohistochemical analysis. Shows no positivity in exocrine glandular cells as expected.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratinocyte differentiation associated protein antibody (ab204583)
Paraffin embedded human liver tissue stained for Keratinocyte differentiation associated protein using ab204583 at 1/2500 dilution in immunohistochemical analysis. Shows no positivity in hepatocytes cells as expected.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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