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AB230175

Anti-Keratocan/KTN antibody

3

(1 Review)

|

(1 Publication)

Rabbit Polyclonal Keratocan/KTN antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KERA.

View Alternative Names

SLRR2B, KERA, Keratocan, KTN, Keratan sulfate proteoglycan keratocan

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratocan/KTN antibody (AB230175)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratocan/KTN antibody (AB230175)

Immunohistochemical analysis of ab230175 diluted at 1 : 50 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labelled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human KERA.

O60938

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Keratocan also known as Kera or Keratan sulfate proteoglycan plays a structural role in connective tissues. This small leucine-rich proteoglycan (SLRP) has an approximate mass of 37 kDa. It is expressed mainly in the cornea but also found in cartilage and other connective tissues. The arrangement of keratocan contributes significantly to the transparency and biomechanical properties of the cornea interacting with collagen fibrils.
Biological function summary

Within the extracellular matrix keratocan maintains corneal clarity and supports tissue organization. It is part of the corneal proteoglycan complex which includes lumican and decorin. These SLRPs collectively regulate collagen fibril assembly and spacing ensuring proper light refraction through the cornea. Keratocan’s presence is also indicative of its role in corneal morphogenesis and repair assisting in wound healing processes.

Pathways

Keratocan interacts with the TGF-β and BMP signaling pathways contributing to matrix assembly and cellular growth. These pathways involve other proteins like fibromodulin and biglycan sharing functions in collagen assembly and tissue repair. TGF-β signaling especially modulates the synthesis of extracellular matrix components during tissue development and healing where keratocan's role becomes important for maintaining structure.

Keratocan relates to corneal dystrophies such as cornea plana and macular dystrophy. Mutations or dysregulation in keratocan can lead to these conditions characterized by opacity and poor vision. During these disease states keratocan interacts with proteins like lumican and collagen type I highlighting the interdependency of these proteins in maintaining corneal integrity. Understanding keratocan's mechanism and interactions helps in developing therapeutic strategies targeting these corneal abnormalities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be important in developing and maintaining corneal transparency and for the structure of the stromal matrix.
See full target information KERA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular medicine (Berlin, Germany) 97:1477-1489 PubMed31399750

2019

Substance P induces fibrotic changes through activation of the RhoA/ROCK pathway in an in vitro human corneal fibrosis model.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Słoniecka,Patrik Danielson
View all publications
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Product promise

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