Anti-KGF/FGF-7 antibody [EPR7261]

• WB

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Western blot - Anti-KGF/FGF-7 antibody [EPR7261] (AB131162)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KGF/FGF-7 antibody [EPR7261] (ab131162) at 2000 µg/mL

All lanes:

Human brain lysates at 15 µg

Predicted band size: 22 kDa

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• WB

Unknown

Western blot - Anti-KGF/FGF-7 antibody [EPR7261] (AB131162)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KGF/FGF-7 antibody [EPR7261] (ab131162) at 0.1 µg/mL

Lane 1:

Mouse brain lysates at 15 µg

Lane 2:

Rat brain lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 22 kDa

Observed band size: 28 kDa

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• WB

Unknown

Western blot - Anti-KGF/FGF-7 antibody [EPR7261] (AB131162)

All lanes:

Western blot - Anti-KGF/FGF-7 antibody [EPR7261] (ab131162) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg

Lane 3:

Fetal kidney lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 22 kDa

Observed band size: 28 kDa

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• WB

CiteAb

Western blot - Anti-KGF/FGF-7 antibody [EPR7261] (AB131162)

KGF/FGF-7 western blot using anti-KGF/FGF-7 antibody [EPR7261] ab131162. Publication image and figure legend from Yang, X., Ma, X., et al., 2020, Stem Cell Res Ther, PubMed 32429994.

ab131162 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab131162 please see the product overview.

The expression of Ang-1, SPC, and KGF under the stimulation of LPS alone and combination of liraglutide for 72 h in three MSCs. a Ang-1, SPC, KGF, b Ang-1, c SPC, and d KGF are expressed at protein or mRNA level under the stimulation of 30 μg/mL LPS alone and combination of 10 nM liraglutide at 72 h. Error bars represent mean ± SD from three independent experiments. Compared with the hCMSCs group stimulated by LPS, *P < 0.05, **P < 0.01, and ***P < 0.001; compared with the LPS group corresponding to MSCs, ++P < 0.01 and +++P < 0.001; compared with the hCMSCs group stimulated by LPS and liraglutide, #P < 0.05, ##P < 0.01, and ###P < 0.001; compared with the hBMSCs group stimulated by LPS and with or without liraglutide, $P < 0.05 and $$$P < 0.001. The survival rate of hCMSCs (e), hBMSCs (f), and hAMSCs (g) in the control group, LPS group, and LPS + liraglutide group. Error bars represent mean ± SD from three independent experiments. Compared with the control group corresponding to time points, *P < 0.05, **P < 0.01, and ***P < 0.001; compared with the LPS group corresponding to time points, +P < 0.05, +++P < 0.001

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