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AB140648

Anti-KHSRP antibody [EPR9865]

4

(4 Reviews)

|

(6 Publications)

Rabbit Recombinant Monoclonal KHSRP antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

FUBP2, KHSRP, Far upstream element-binding protein 2, FUSE-binding protein 2, KH type-splicing regulatory protein, p75, KSRP

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (AB140648)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (AB140648)

Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab140648 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (AB140648)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (AB140648)

Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using ab140648 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (AB140648)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (AB140648)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab140648 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (AB140648)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (AB140648)

Immunohistochemical analysis of paraffin embedded Human brain tissue labelling KHSRP with ab140648 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (AB140648)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (AB140648)

Immunohistochemical analysis of paraffin embedded Human colon tissue labelling KHSRP with ab140648 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (AB140648)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (AB140648)

Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab140648 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-KHSRP antibody [EPR9865] (AB140648)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-KHSRP antibody [EPR9865] (AB140648)

Overlay histogram showing Hela cells stained with ab140648 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140648, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (AB140648)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (AB140648)

Immunohistochemical analysis of paraffin embedded Normal Human Uterus tissue using ab140648 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-KHSRP antibody [EPR9865] (AB140648)
  • WB

Unknown

Western blot - Anti-KHSRP antibody [EPR9865] (AB140648)

All lanes:

Western blot - Anti-KHSRP antibody [EPR9865] (ab140648) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

A673 cell lysate at 10 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 73 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9865

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody (AB264265)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/1000", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
See full target information KHSRP
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:30217 PubMed40825800

2025

The regulation of miR-155 strand selection by CELF2, FUBP1 and KSRP proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Jeff S J Yoon,Thomas C Chamberlain,Nada Lallous,Alice L-F Mui

Stem cell research & therapy 11:504 PubMed33246498

2020

Investigation of de novo mutations in a schizophrenia case-parent trio by induced pluripotent stem cell-based in vitro disease modeling: convergence of schizophrenia- and autism-related cellular phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Edit Hathy,Eszter Szabó,Nóra Varga,Zsuzsa Erdei,Csongor Tordai,Boróka Czehlár,Máté Baradits,Bálint Jezsó,Júlia Koller,László Nagy,Mária Judit Molnár,László Homolya,Zsófia Nemoda,Ágota Apáti,János M Réthelyi

Journal of dairy science 103:12091-12103 PubMed33010914

2020

Ruminal epithelial cell proliferation and short-chain fatty acid transporters in vitro are associated with abundance of period circadian regulator 2 (PER2).

Applications

Unspecified application

Species

Unspecified reactive species

Jian Gao,Qiaoyun Xu,Mengzhi Wang,Jialiang Ouyang,Wen Tian,Dan Feng,Yusheng Liang,Beibei Jiang,Juan J Loor

Advanced science (Weinheim, Baden-Wurttemberg, Ger 7:2001800 PubMed33101872

2020

E3 Ligase FBXW2 Is a New Therapeutic Target in Obesity and Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Wang,Wenjing Xu,Yuelin Chao,Minglu Liang,Fengxiao Zhang,Kai Huang

PloS one 15:e0231639 PubMed32324763

2020

Interleukin-10 control of pre-miR155 maturation involves CELF2.

Applications

Unspecified application

Species

Unspecified reactive species

Jeff S J Yoon,Mike K Wu,Tian Hao Zhu,Helen Zhao,Sylvia T Cheung,Thomas C Chamberlain,Alice L-F Mui

Oncotarget 11:131-147 PubMed32010427

2020

KHSRP-bound small nucleolar RNAs associate with promotion of cell invasiveness and metastasis of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Keisuke Taniuchi,Mitsunari Ogasawara
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com