Anti-Ki67 antibody [37C7-12] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (AB252260)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Ki67 with ab245113 at 1/100 diluton, followed by a Goat Anti-Mouse IgG (Alexa Fluor^® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolus staining on HeLa cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows :
-ve control 1 : ab245113 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2 : ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary at 1/1000 dilution.

This image was produced using the same antibody clone but in a different formulation ab245113, PBS, sodium azide, glycerol and BSA.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (AB252260)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Ki67 with ab245113 at 1/800 diluton, followed by a ready to use secondary. Nuclear staining on human tonsil tissue is observed. The section was incubated with ab245113 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This image was produced using the same antibody clone but in a different formulation ab245113, PBS, sodium azide, glycerol and BSA.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (AB252260)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab245113)

ab245113 staining Ki67 in Hap1-Ki67 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab245113 at 1μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 4% paraformaldehyde (10 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (AB252260)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HAP1 (human chronic myelogenous leukemia near-haploid cell line) cells labeling Ki67 with ab245113 at 1/100 diluton, followed by a Goat Anti-Mouse IgG (Alexa Fluor^® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolus staining on HAP1 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows :
-ve control 1 : ab245113 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2 : ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary at 1/1000 dilution.

This image was produced using the same antibody clone but in a different formulation ab245113, PBS, sodium azide, glycerol and BSA.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (AB252260)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Ki67 with ab245113 at 1/800 diluton, followed by a ready to use secondary. Nuclear staining on human breast carcinoma tissue is observed. The section was incubated with ab245113 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This image was produced using the same antibody clone but in a different formulation PBS, sodium azide, glycerol and BSA (ab245113).