Mouse Recombinant Monoclonal Ki67 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (AB252260), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-37c7-12-bsa-and-azide-free-ab252260--immunocytochemistry-immunofluorescence-img173668.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (AB252260)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (AB252260), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-37c7-12-bsa-and-azide-free-ab252260--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61040.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (AB252260)")
![Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (AB252260), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-37c7-12-bsa-and-azide-free-ab252260--immunocytochemistry-immunofluorescence-img76579.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (AB252260)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (AB252260), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-37c7-12-bsa-and-azide-free-ab252260--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119009.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (AB252260)")
![Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (AB252260), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-37c7-12-bsa-and-azide-free-ab252260--immunocytochemistry-immunofluorescence-img73071.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (AB252260)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt (Intra) | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes If fixing cells in 4% PFA (20 min, room temp), it is recommended to permeabilized cells with 0.1% Triton-X for 5 min. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
6 products for Alternative Product
1 product for Alternative Version
Target data
Function
The protein expressed by the MKI67 gene is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly and associates with the surface of the mitotic chromosome, specifically the perichromosomal layer, covering a significant portion of the chromosome surface (PubMed:27362226). It prevents chromosomes from collapsing into a single chromatin mass by creating a steric and electrostatic charge barrier due to its high net electrical charge, acting as a surfactant that disperses chromosomes and enables their independent motility (PubMed:27362226). The protein binds DNA, with a preference for supercoiled and AT-rich DNA (PubMed:10878551), and may play a role in chromatin organization, though it is unclear if it directly influences chromatin organization or if this is an indirect result of its function in maintaining dispersed mitotic chromosomes (Probable, PubMed:24867636). This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67
Recommended products
Mouse Recombinant Monoclonal Ki67 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 37C7-12
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab252260 is the carrier-free version of Anti-Ki67 antibody [37C7-12] ab245113.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.
- Biological function summary
The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.
- Pathways
Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.
- Associated diseases and disorders
Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-37c7-12-bsa-and-azide-free-ab252260--immunocytochemistry-immunofluorescence-img173668.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)")
Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-Ki67 antibody [37C7-12] ab245113)
Anti-Ki67 antibody [37C7-12] ab245113 staining Ki67 in Hap1-Ki67 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Anti-Ki67 antibody [37C7-12] ab245113 at 1μg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-37c7-12-bsa-and-azide-free-ab252260--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61040.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Ki67 with Anti-Ki67 antibody [37C7-12] ab245113 at 1/800 diluton, followed by a ready to use secondary. Nuclear staining on human breast carcinoma tissue is observed. The section was incubated with Anti-Ki67 antibody [37C7-12] ab245113 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.This image was produced using the same antibody clone but in a different formulation PBS, sodium azide, glycerol and BSA (Anti-Ki67 antibody [37C7-12] ab245113).
![Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-37c7-12-bsa-and-azide-free-ab252260--immunocytochemistry-immunofluorescence-img76579.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)")
Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Ki67 with Anti-Ki67 antibody [37C7-12] ab245113 at 1/100 diluton, followed by a Goat Anti-Mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolus staining on HeLa cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-beta IV Tubulin antibody [EPR16775] ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-Ki67 antibody [37C7-12] ab245113 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-beta IV Tubulin antibody [EPR16775] ab179504 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.This image was produced using the same antibody clone but in a different formulation Anti-Ki67 antibody [37C7-12] ab245113, PBS, sodium azide, glycerol and BSA.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-37c7-12-bsa-and-azide-free-ab252260--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119009.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Ki67 with Anti-Ki67 antibody [37C7-12] ab245113 at 1/800 diluton, followed by a ready to use secondary. Nuclear staining on human tonsil tissue is observed. The section was incubated with Anti-Ki67 antibody [37C7-12] ab245113 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.This image was produced using the same antibody clone but in a different formulation Anti-Ki67 antibody [37C7-12] ab245113, PBS, sodium azide, glycerol and BSA.
![Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-37c7-12-bsa-and-azide-free-ab252260--immunocytochemistry-immunofluorescence-img73071.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)")
Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HAP1 (human chronic myelogenous leukemia near-haploid cell line) cells labeling Ki67 with Anti-Ki67 antibody [37C7-12] ab245113 at 1/100 diluton, followed by a Goat Anti-Mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolus staining on HAP1 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-beta IV Tubulin antibody [EPR16775] ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-Ki67 antibody [37C7-12] ab245113 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-beta IV Tubulin antibody [EPR16775] ab179504 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.This image was produced using the same antibody clone but in a different formulation Anti-Ki67 antibody [37C7-12] ab245113, PBS, sodium azide, glycerol and BSA.
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