Chicken Polyclonal Ki67 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MKI67.
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- Cell death & disease 14:2642023USP1 modulates hepatocellular carcinoma progression via the Hippo/TAZ axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongyi Liu,Quanhui Li,Yifeng Zang,Xin Li,Zhongbo Li,Peng Zhang,Chang Feng,Penghe Yang,Jiayao Cui,Yanan Sun,Tian Wei,Peng Su,Xin Zhao,Huijie Yang,Yinlu DingPubMed 37041150
- Thoracic cancer 14:881-8922023Circ_0001667 accelerates breast cancer proliferation and angiogenesis through regulating CXCL10 expression by sponging miR-6838-5p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu Zhang,Jiami Huang,Jiayun Wang,Yongheng Li,Guohua Hu,He LiPubMed 36811283
- Frontiers in oncology 12:9061942022The cyclin dependent kinase inhibitor p21 is a therapeutic target in high-risk neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgnes Luise Sorteberg,Vesa Halipi,Malin Wickström,Shahrzad Shirazi FardPubMed 36147919
- Oncology letters 22:7732021Long non-coding RNA TMCC1-AS1 predicts poor prognosis and accelerates epithelial-mesenchymal transition in liver cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Chen,Na Su,Guiying Li,Yanfeng Shen,Xiaoting DuanPubMed 34589152
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e20048462021SPOP and OTUD7A Control EWS-FLI1 Protein Stability to Govern Ewing Sarcoma Growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiyuan Su,Jianfeng Chen,Yao Jiang,Ying Wang,Tamara Vital,Jiaming Zhang,Christian Laggner,Kong T Nguyen,Zhichuan Zhu,Alex W Prevatte,Natalie K Barker,Laura E Herring,Ian J Davis,Pengda LiuPubMed 34060252
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
Preservative: 0.065% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human MKI67. Database link P46013
Reactivity data
Select an application| ICC | WB | |
|---|---|---|
| Human | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
Associated Products
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Target data
Function
The protein expressed by the MKI67 gene is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly and associates with the surface of the mitotic chromosome, specifically the perichromosomal layer, covering a significant portion of the chromosome surface (PubMed:27362226). It prevents chromosomes from collapsing into a single chromatin mass by creating a steric and electrostatic charge barrier due to its high net electrical charge, acting as a surfactant that disperses chromosomes and enables their independent motility (PubMed:27362226). The protein binds DNA, with a preference for supercoiled and AT-rich DNA (PubMed:10878551), and may play a role in chromatin organization, though it is unclear if it directly influences chromatin organization or if this is an indirect result of its function in maintaining dispersed mitotic chromosomes (Probable, PubMed:24867636). This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67
Recommended products
Chicken Polyclonal Ki67 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MKI67.
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
Preservative: 0.065% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human MKI67. Database link P46013
- Purity
- IgY fraction
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.
- Biological function summary
The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.
- Pathways
Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.
- Associated diseases and disorders
Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry - Anti-Ki67 antibody (ab254123)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ki67 (red) using ab254123 at 1/1000 dilution in ICC. Beta tubulin is stained (green). Nuclei are shown in blue.
Western blot - Anti-Ki67 antibody (ab254123)
All lanes: Western blot - Anti-Ki67 antibody (ab254123) at 1/5000 dilution
All lanes: T-47D (human ductal breast epithelial tumor cell line) (serum starved / re-fed for 2.5 hours) cell lysate
Predicted band size: 358 kDa
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