AB264429

Anti-Ki67 antibody

Name: Anti-Ki67 antibody (ab264429) | Abcam
Brand: Abcam Limited
SKU: AB264429
Price: 525 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal Ki67 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Mouse Mki67 aa 1650-1700.

View Alternative Names

Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67 homolog, Antigen KI-67 homolog, Antigen Ki67 homolog, Mki67

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB264429)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB264429)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse peyer's patch tissue labeling Ki67 with ab264429 at 1/250 dilution. Detection : DAB.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB264429)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse intestine tissue labeling Ki67 with ab264429 at 1/250 dilution. Detection : DAB.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB264429)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse spleen tissue labeling Ki67 with ab264429 at 1/250 dilution. Detection : DAB.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB264429)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse intestine tissue labeling Ki67 with ab264429 at 1/250 dilution. Detection : DAB.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Mki67 aa 1650-1700. The exact immunogen used to generate this antibody is proprietary information.

E9PVX6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.

Biological function summary

The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.

Pathways

Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.

Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed : 27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed : 27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier : the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed : 27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity). Does not contribute to the internal structure of mitotic chromosomes (PubMed : 26949251). May play a role in chromatin organization (PubMed : 26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.

See full target information Mki67

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1273182 PubMed38053996

2023

Epidermal keratinocyte-specific STAT3 deficiency aggravated atopic dermatitis-like skin inflammation in mice through TSLP upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao-Yuan Wang,Yu-Xin Zheng,Fan Xu,Ying-Zhe Cui,Xue-Yan Chen,Si-Qi Chen,Bing-Xi Yan,Yuan Zhou,Min Zheng,Xiao-Yong Man

International journal of molecular sciences 23: PubMed35682969

2022

Identification of Structural and Molecular Signatures Mediating Adaptive Changes in the Mouse Kidney in Response to Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge Lopez-Tello,Maria Angeles Jimenez-Martinez,Esteban Salazar-Petres,Ritik Patel,Amy L George,Richard G Kay,Amanda N Sferruzzi-Perri

Journal of immunology research 2022:1917585 PubMed35692504

2022

Asiaticoside Suppresses Gastric Cancer Progression and Induces Endoplasmic Reticulum Stress through the miR-635/HMGA1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Zhang,Xiaolin Ji,Zhenguang Chen,Zhichao Yao

Pharmaceutical biology 60:764-773 PubMed35387566

2022

Caudatin blocks the proliferation, stemness and glycolysis of non-small cell lung cancer cells through the Raf/MEK/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Hou,Qing Chen,Yufeng Huang,Zhiwei Wu,De Ma

Development (Cambridge, England) 149: PubMed34982814

2022

Loss of imprinting of the Igf2-H19 ICR1 enhances placental endocrine capacity via sex-specific alterations in signalling pathways in the mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Bethany R L Aykroyd,Simon J Tunster,Amanda N Sferruzzi-Perri

Gut microbes 11:1385-1404 PubMed32515658

2020

The protective effect of G9-1 against mucus degradation by following small intestine injury caused by a proton pump inhibitor and aspirin.

Applications

Unspecified application

Species

Unspecified reactive species

Tsutomu Yoshihara,Yosuke Oikawa,Takayuki Kato,Takaomi Kessoku,Takashi Kobayashi,Shingo Kato,Noboru Misawa,Keiichi Ashikari,Akiko Fuyuki,Hidenori Ohkubo,Takuma Higurashi,Yoko Tateishi,Yoshiki Tanaka,Shunji Nakajima,Hiroshi Ohno,Koichiro Wada,Atsushi Nakajima

View all publications

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