Mouse Recombinant Monoclonal Ki67 antibody. Carrier free. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (AB279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41020.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (AB279657)")
![Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [B56] - BSA and Azide free (AB279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunocytochemistry-immunofluorescence-img168535.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [B56] - BSA and Azide free (AB279657)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (AB279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87089.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (AB279657)")
![Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [B56] - BSA and Azide free (AB279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunocytochemistry-immunofluorescence-img68342.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [B56] - BSA and Azide free (AB279657)")
![Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [B56] - BSA and Azide free (AB279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunocytochemistry-immunofluorescence-img91600.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [B56] - BSA and Azide free (AB279657)")
Publications
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- Reproductive biology and endocrinology : RB&E 21:492023Artificial ovaries constructed from biodegradable chitin-based hydrogels with the ability to restore ovarian endocrine function and alleviate osteoporosis in ovariectomized mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDu Xiang,Encheng Zhou,Mei Wang,Kan Wang,Shujun Zhou,Qing Ma,Zibiao Zhong,Qifa Ye,Yun Chen,Xiaoli Fan,Yanfeng WangPubMed 37208699
- Veterinary medicine and science 9:1426-14372023Evaluation of the in vitro and in vivo effect of liposomal doxorubicin along with oncolytic Newcastle disease virus on 4T1 cell line: Animal preclinical research.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPooya Faranoush,Alireza Jahandideh,Reza Nekouian,Pejman MortazaviPubMed 36920334
- Cancer medicine 10:7475-74912021Positive electrostatic therapy of metastatic tumors: selective induction of apoptosis in cancer cells by pure charges.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshkan Zandi,Saeid Rafizadeh-Tafti,Fatemeh Shojaeian,Mohammad Ali Khayamian,Fereshteh Abbasvandi,Mohammad Faranoush,Robab Anbiaee,Sahar Najafikhoshnoo,Parisa Hoseinpour,Sepanta Assadi,Pouyan Katebi,Zahra Davari Sh,Shahriar Shalileh,Mohammad Salemizadeh Parizi,Shohreh Vanaei,Mohammadreza Ghaderinia,Hamed Abadijoo,Payam Taheri,Mohammad Reza Esmailinejad,Hassan Sanati,Mohammad Reza Rostami,Reza Sadeghian,Yasin Kordehlachin,S M Sadegh Mousavi-Kiasary,Amir Mamdouh,Seyyed Hossein Miraghaie,Hossein Baharvand,Mohammad AbdolahadPubMed 34626092
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested |
| Mouse | Tested | Expected | Not recommended | Expected |
| Rat | Tested | Expected | Not recommended | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
Target data
Function
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Alternative names
Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67
Recommended products
Mouse Recombinant Monoclonal Ki67 antibody. Carrier free. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.
Alternative names
Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
B56
- Purification technique
Affinity purification Protein A
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab279657 is the carrier-free version of Anti-Ki67 antibody [B56] ab279653.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.
- Biological function summary
The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.
- Pathways
Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.
- Associated diseases and disorders
Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
15 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41020.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)
This data was developed using Anti-Ki67 antibody [B56] ab279653 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Ki67 with Anti-Ki67 antibody [B56] ab279653 at 1/1000 dilution (0.936 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human colon. The section was incubated with Anti-Ki67 antibody [B56] ab279653 for 30 mins at room temperature and followed by mouse specific IgG antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
![Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunocytochemistry-immunofluorescence-img168535.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)
This data was developed using Anti-Ki67 antibody [B56] ab279653 the same antibody clone in a different buffer formulation.
Anti-Ki67 antibody [B56] ab279653 staining Ki67 in wild-type Hap1 cells, with negative expression in Ki67 knockout Hap1 cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-Ki67 antibody [B56] ab279653 at 5 μg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin at 1 μg/ml. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
This product also work with 4% formaldehyde (10 min) fixation under the same testing conditions.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87089.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)
This data was developed using Anti-Ki67 antibody [B56] ab279653 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Ki67 with Anti-Ki67 antibody [B56] ab279653 at 1/1000 dilution (0.936 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human ovarian carcinoma. The section was incubated with Anti-Ki67 antibody [B56] ab279653 for 30 mins at room temperature and followed by mouse specific IgG antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
![Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunocytochemistry-immunofluorescence-img68342.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)
This data was developed using Anti-Ki67 antibody [B56] ab279653 the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Ki67 with Anti-Ki67 antibody [B56] ab279653 at 1/50 dilution (18.72 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nucleolar staining in HeLa cell line. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution was used to counterstain tubulin (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunocytochemistry-immunofluorescence-img91600.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)
This data was developed using Anti-Ki67 antibody [B56] ab279653 the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized wild-type HeLa (Human cervix adenocarcinoma epithelial cell) cells and KI67 KO HeLa cells labelling Ki67 with Anti-Ki67 antibody [B56] ab279653 at 1/50 dilution (18.72 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nucleolar staining in Parental HeLa cell line and no staining in MKI67 HeLa KO cell line. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution was used to counterstain tubulin (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Flow Cytometry (Intracellular) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--flow-cytometry-intracellular-img12507.jpg "Flow Cytometry (Intracellular) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
Flow Cytometry (Intracellular) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)
This data was developed using Anti-Ki67 antibody [B56] ab279653 the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Ki67 with Anti-Ki67 antibody [B56] ab279653 at 1/1000 dilution (0.1ug)/ Right compared with a Mouse monoclonal IgG / Left isotype control. A Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution was used as the secondary antibody.
![Flow Cytometry (Intracellular) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--flow-cytometry-intracellular-img13164.jpg "Flow Cytometry (Intracellular) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
Flow Cytometry (Intracellular) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)
This data was developed using Anti-Ki67 antibody [B56] ab279653 the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Parental HeLa (Human cervix adenocarcinoma epithelial cells) (Right)/ MKI67 KO HeLa cells (Left) labelling Ki67 with Anti-Ki67 antibody [B56] ab279653 at 1/1000 dilution (0.1ug) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81528.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)
This data was developed using Anti-Ki67 antibody [B56] ab279653 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Ki67 with Anti-Ki67 antibody [B56] ab279653 at 1/1000 dilution (0.936 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse colon. The section was incubated with Anti-Ki67 antibody [B56] ab279653 for 30 mins at room temperature and followed by mouse specific IgG antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img30550.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)
This data was developed using Anti-Ki67 antibody [B56] ab279653 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse breast carcinoma tissue labeling Ki67 with Anti-Ki67 antibody [B56] ab279653 at 1/1000 dilution (0.936 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse breast carcinoma. The section was incubated with Anti-Ki67 antibody [B56] ab279653 for 30 mins at room temperature and followed by mouse specific IgG antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117686.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)
This data was developed using Anti-Ki67 antibody [B56] ab279653 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Ki67 with Anti-Ki67 antibody [B56] ab279653 at 1/1000 dilution (0.936 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat colon. The section was incubated with Anti-Ki67 antibody [B56] ab279653 for 30 mins at room temperature and followed by mouse specific IgG antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img438093.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)
This data was developed using Anti-Ki67 antibody [B56] ab279653, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Ki67 with Anti-Ki67 antibody [B56] ab279653 at 1/1000 dilution. The section was incubated with Anti-Ki67 antibody [B56] ab279653 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI. Secondary antibody only control: Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 1/1000 dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution1) for 40 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img438094.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)
This data was developed using Anti-Ki67 antibody [B56] ab279653, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Ki67 with Anti-Ki67 antibody [B56] ab279653 at 1/1000 dilution. The section was incubated with Anti-Ki67 antibody [B56] ab279653 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI. Secondary antibody only control: Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 1/1000 dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution1) for 40 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img439659.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)
This data was developed using Anti-Ki67 antibody [B56] ab279653, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of formalin fixed paraffin embedded human colon labelling Ki67 with Anti-Ki67 antibody [B56] ab279653 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.
Anti-Ki67 antibody [B56] ab279653 Anti-Ki67 antibody [B56] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img438095.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)
This data was developed using Anti-Ki67 antibody [B56] ab279653, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Ki67 with Anti-Ki67 antibody [B56] ab279653 at 1/1000 dilution. The section was incubated with Anti-Ki67 antibody [B56] ab279653 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI. Secondary antibody only control: Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 1/1000 dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution1) for 40 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img438096.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)
This data was developed using Anti-Ki67 antibody [B56] ab279653, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Ki67 with Anti-Ki67 antibody [B56] ab279653 at 1/1000 dilution. The section was incubated with Anti-Ki67 antibody [B56] ab279653 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI. Secondary antibody only control: Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 1/1000 dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution1) for 40 mins.
![Flow Cytometry (Intracellular) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--flow-cytometry-intracellular-img12507.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
![Flow Cytometry (Intracellular) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--flow-cytometry-intracellular-img13164.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81528.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img30550.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117686.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img438093.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img438094.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img439659.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img438095.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657), expandable thumbnail](https://content.abcam.com/products/images/ki67-antibody-b56-bsa-and-azide-free-ab279657--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img438096.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [B56] - BSA and Azide free (ab279657)")
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