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Mouse Monoclonal Ki67 antibody. Suitable for IHC-P, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MKI67 aa 2250-2500.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2462] (AB238019), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2462] (AB238019), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2462] (AB238019), expandable thumbnail
  • Protein Array - Anti-Ki67 antibody [MKI67/2462] (AB238019), expandable thumbnail

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human MKI67 aa 2250-2500. The exact immunogen used to generate this antibody is proprietary information. Database link P46013

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PProtein Array
Human
Tested
Expected
Recombinant full length protein - Human
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

Incubate with primary antibody for 30 minutes at RT

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

7 products for Alternative Product

Target data

Function

The protein expressed by the MKI67 gene is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly and associates with the surface of the mitotic chromosome, specifically the perichromosomal layer, covering a significant portion of the chromosome surface (PubMed:27362226). It prevents chromosomes from collapsing into a single chromatin mass by creating a steric and electrostatic charge barrier due to its high net electrical charge, acting as a surfactant that disperses chromosomes and enables their independent motility (PubMed:27362226). The protein binds DNA, with a preference for supercoiled and AT-rich DNA (PubMed:10878551), and may play a role in chromatin organization, though it is unclear if it directly influences chromatin organization or if this is an indirect result of its function in maintaining dispersed mitotic chromosomes (Probable, PubMed:24867636). This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

Recommended products

Mouse Monoclonal Ki67 antibody. Suitable for IHC-P, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MKI67 aa 2250-2500.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human MKI67 aa 2250-2500. The exact immunogen used to generate this antibody is proprietary information. Database link P46013
Clone number
MKI67/2462
Purification technique
Affinity purification Protein A/G
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from Bioreactor Concentrate by Protein A/G.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.

Biological function summary

The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.

Pathways

Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.

Associated diseases and disorders

Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.

Product promise

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4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2462] (ab238019), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2462] (ab238019)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for Ki67 using ab238019 at 2 μg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2462] (ab238019), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2462] (ab238019)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for Ki67 using ab238019 at 2 μg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2462] (ab238019), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2462] (ab238019)

    Formalin-fixed, paraffin-embedded human tonsil-skin tissue stained for Ki67 using ab238019 at 2 μg/ml in immunohistochemical analysis.

  • Protein Array - Anti-Ki67 antibody [MKI67/2462] (ab238019), expandable thumbnail

    Protein Array - Anti-Ki67 antibody [MKI67/2462] (ab238019)

    Analysis of Protein Array containing more than 19,000 full-length human proteins using ab238019.


    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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