Anti-Ki67 antibody [MKI67/2465]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2465] (AB238020)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Ki67 using ab238020 at 2 μg/ml in immunohistochemical analysis.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Ki67 antibody [MKI67/2465] (AB238020)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Ki67 with ab238020 at 2 μg per 10⁶ cells (blue) compared to an isotype control (red). A Goat Anti-mouse CF488 secondary antibody was used.

• ICC

Supplier Data

Immunocytochemistry - Anti-Ki67 antibody [MKI67/2465] (AB238020)

MCF7 (human breast adenocarcinoma cell line) cells stained for Ki67 (green) using ab238020 at 2 μg/mL in ICC. The membrane is labelled with phalloidin (red).

• ICC

AbReview70386****

Immunocytochemistry - Anti-Ki67 antibody [MKI67/2465] (AB238020)

Immunocytochemistry analysis of paraformaldehyde-fixed human hES cells differentiated into neural crest cells permeabilized with 0.5% Triton, staining with ab238020 at 1/100 dilution. Secondary antibody was Alexa Fluor™ 647 Goat anti Mouse at 1/500 dilution. Cells were incubated with the primary antibody with 10% serum in PBS for 18 hours at 4°C . Blocking was done using 10% serum for 2 hours at room 25°C.

This image is courtesy of an Abreview submitted by Ingrid Saldana

• ICC

AbReview69928****

Immunocytochemistry - Anti-Ki67 antibody [MKI67/2465] (AB238020)

Immunocytochemistry analysis of formaldehyde-fixed human hiSPC cells, staining with ab238020 at 1/100 dilution. Secondary antibody was Alexa Fluor® 647 goat anti mouse 1/1000 dilution. Cells were incubated with the primary antibody with 1% BSA 0.3% Triton X for 12 hours at 4°C . Blocking was done using 3% BSA for 1 hour.

This image is courtesy of an anonymous Abreview

• Protein Array

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Protein Array - Anti-Ki67 antibody [MKI67/2465] (AB238020)

ab238020 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.