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Anti-Ki67 antibody [OTI5D7] (ab156956) is a rat monoclonal antibody that is used to detect Ki67 in Flow Cytometry (Intra), Flow Cytometry, IHC-P. Suitable for Human samples.



- Over 40 publications


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (AB156956), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (AB156956), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (AB156956), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Ki67 antibody [OTI5D7] (AB156956), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (AB156956), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Rat
Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human MKI67 aa 1150-1500. The exact immunogen used to generate this antibody is proprietary information. Database link P46013

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow Cyt (Intra)
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/150
Notes

Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120℃ for 3min.

Tested
Tested

Species
Human
Dilution info
1/100
Notes

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

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Target data

Function

The protein expressed by the MKI67 gene is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly and associates with the surface of the mitotic chromosome, specifically the perichromosomal layer, covering a significant portion of the chromosome surface (PubMed:27362226). It prevents chromosomes from collapsing into a single chromatin mass by creating a steric and electrostatic charge barrier due to its high net electrical charge, acting as a surfactant that disperses chromosomes and enables their independent motility (PubMed:27362226). The protein binds DNA, with a preference for supercoiled and AT-rich DNA (PubMed:10878551), and may play a role in chromatin organization, though it is unclear if it directly influences chromatin organization or if this is an indirect result of its function in maintaining dispersed mitotic chromosomes (Probable, PubMed:24867636). This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

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Anti-Ki67 antibody [OTI5D7] (ab156956) is a rat monoclonal antibody that is used to detect Ki67 in Flow Cytometry (Intra), Flow Cytometry, IHC-P. Suitable for Human samples.



- Over 40 publications

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human MKI67 aa 1150-1500. The exact immunogen used to generate this antibody is proprietary information. Database link P46013
Clone number
OTI5D7
Purification technique
Affinity purification Protein A
Concentration
Loading...
Purification notes

ab156956 was purified from cell culture supernatant by affinity chromatography.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Ki67 antibody [OTI5D7] (ab156956) is a rat monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.

Trusted by the scientific community


Anti-Ki67 [OTI5D7] (ab156956) was first used in a scientific publication in 2013 and has been cited over 40 times in peer-reviewed journals.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.

Biological function summary

The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.

Pathways

Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.

Associated diseases and disorders

Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.

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9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956)

    Immunohistochemical analysis of paraffin-embedded Human lymphoma tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956)

    Immunohistochemical analysis of paraffin-embedded Human breast adenocarcinoma tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.

  • Flow Cytometry (Intracellular) - Anti-Ki67 antibody [OTI5D7] (ab156956), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Ki67 antibody [OTI5D7] (ab156956)

    Overlay histogram showing Ramos cells stained with ab156956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab156956, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956)

    Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956)

    Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956)

    Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [OTI5D7] (ab156956)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.

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